Effects of supplementation of DL-methionine on tissue and plasma antioxidant status during heat-induced oxidative stress in broilers
Exposure to high ambient temperature has been shown to impair growth performance and to cause oxidative stress in broilers. This study investigated the hypothesis that supplementation with methionine (Met) as DL-Met (DLM) more than the National Research Council recommendations improves growth perfor...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579120306295 |
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| author | Johanna O. Zeitz Anne Fleischmann Tamara Ehbrecht Erika Most Silvia Friedrichs Rose Whelan Denise K. Gessner Klaus Failing Dieter Lütjohann Klaus Eder |
| author_facet | Johanna O. Zeitz Anne Fleischmann Tamara Ehbrecht Erika Most Silvia Friedrichs Rose Whelan Denise K. Gessner Klaus Failing Dieter Lütjohann Klaus Eder |
| author_sort | Johanna O. Zeitz |
| collection | DOAJ |
| description | Exposure to high ambient temperature has been shown to impair growth performance and to cause oxidative stress in broilers. This study investigated the hypothesis that supplementation with methionine (Met) as DL-Met (DLM) more than the National Research Council recommendations improves growth performance and alleviates oxidative stress in broilers exposed to high ambient temperature. One-day-old male Cobb-500 broilers (n = 68) were allotted to 4 groups and phase-fed 3 basal diets during days 1 to 10, 11 to 21, and 22 to 35. One group was kept under thermoneutral temperature conditions and received the basal diets with Met + cysteine (Cys) concentrations according to recommendations of NRC. The other 3 groups were kept in a room with an increased ambient temperature from week 3 to 5 and were fed either the basal diet or the basal diets supplemented with 2 levels of DLM in which Met + Cys concentrations exceeded NRC recommendations by around 20% (group DLM1) and 40% (group DLM2), respectively. As expected, the broilers exposed to high ambient temperature showed a lower feed intake, lower body weight gains, a higher feed:gain ratio, and biochemical indications of oxidative stress in comparison to broilers kept under thermoneutral temperature conditions. Supplementation of DLM did not improve the growth performance in broilers exposed to high ambient temperature. However, the broilers supplemented with DLM had increased concentrations of glutathione in liver and breast muscle (groups DLM1 and DLM2), increased concentrations of tocopherols in the liver (group DLM2), and reduced concentrations of 7α-hydroxycholesterol and 7-ketocholesterol in heat-processed thigh muscle (groups DLM1 and DLM2) in comparison to the control group exposed to high ambient temperature. Concentrations of thiobarbituric acid-reactive substances and vitamin C in plasma, liver, and muscle were not different between the 3 groups exposed to heat stress. Nevertheless, the study shows that supplementation of DLM in slight excess of the Met concentration required for maximum growth performance improved the antioxidant status in tissues and reduced the susceptibility of muscle toward oxidation in heat-stressed broilers. |
| first_indexed | 2024-12-16T09:18:15Z |
| format | Article |
| id | doaj.art-33e67004a1b944fa939600dcc278534d |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-16T09:18:15Z |
| publishDate | 2020-12-01 |
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| series | Poultry Science |
| spelling | doaj.art-33e67004a1b944fa939600dcc278534d2022-12-21T22:36:50ZengElsevierPoultry Science0032-57912020-12-01991268376847Effects of supplementation of DL-methionine on tissue and plasma antioxidant status during heat-induced oxidative stress in broilersJohanna O. Zeitz0Anne Fleischmann1Tamara Ehbrecht2Erika Most3Silvia Friedrichs4Rose Whelan5Denise K. Gessner6Klaus Failing7Dieter Lütjohann8Klaus Eder9University of Giessen, Institute of Animal Nutrition and Nutritional Physiology, Justus-Liebig-University, Giessen, GermanyUniversity of Giessen, Institute of Animal Nutrition and Nutritional Physiology, Justus-Liebig-University, Giessen, GermanyUniversity of Giessen, Institute of Animal Nutrition and Nutritional Physiology, Justus-Liebig-University, Giessen, GermanyUniversity of Giessen, Institute of Animal Nutrition and Nutritional Physiology, Justus-Liebig-University, Giessen, GermanyInstitute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, Bonn, GermanyEvonik Operations GmbH, Hanau-Wolfgang, GermanyUniversity of Giessen, Institute of Animal Nutrition and Nutritional Physiology, Justus-Liebig-University, Giessen, Germany; Corresponding author:Unit of Biomathematics and Data Processing, Faculty of Veterinary Medicine, Justus-Liebig-University, Giessen, GermanyInstitute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, Bonn, GermanyUniversity of Giessen, Institute of Animal Nutrition and Nutritional Physiology, Justus-Liebig-University, Giessen, GermanyExposure to high ambient temperature has been shown to impair growth performance and to cause oxidative stress in broilers. This study investigated the hypothesis that supplementation with methionine (Met) as DL-Met (DLM) more than the National Research Council recommendations improves growth performance and alleviates oxidative stress in broilers exposed to high ambient temperature. One-day-old male Cobb-500 broilers (n = 68) were allotted to 4 groups and phase-fed 3 basal diets during days 1 to 10, 11 to 21, and 22 to 35. One group was kept under thermoneutral temperature conditions and received the basal diets with Met + cysteine (Cys) concentrations according to recommendations of NRC. The other 3 groups were kept in a room with an increased ambient temperature from week 3 to 5 and were fed either the basal diet or the basal diets supplemented with 2 levels of DLM in which Met + Cys concentrations exceeded NRC recommendations by around 20% (group DLM1) and 40% (group DLM2), respectively. As expected, the broilers exposed to high ambient temperature showed a lower feed intake, lower body weight gains, a higher feed:gain ratio, and biochemical indications of oxidative stress in comparison to broilers kept under thermoneutral temperature conditions. Supplementation of DLM did not improve the growth performance in broilers exposed to high ambient temperature. However, the broilers supplemented with DLM had increased concentrations of glutathione in liver and breast muscle (groups DLM1 and DLM2), increased concentrations of tocopherols in the liver (group DLM2), and reduced concentrations of 7α-hydroxycholesterol and 7-ketocholesterol in heat-processed thigh muscle (groups DLM1 and DLM2) in comparison to the control group exposed to high ambient temperature. Concentrations of thiobarbituric acid-reactive substances and vitamin C in plasma, liver, and muscle were not different between the 3 groups exposed to heat stress. Nevertheless, the study shows that supplementation of DLM in slight excess of the Met concentration required for maximum growth performance improved the antioxidant status in tissues and reduced the susceptibility of muscle toward oxidation in heat-stressed broilers.http://www.sciencedirect.com/science/article/pii/S0032579120306295methionineheat stressoxidative stressantioxidant responsebroiler |
| spellingShingle | Johanna O. Zeitz Anne Fleischmann Tamara Ehbrecht Erika Most Silvia Friedrichs Rose Whelan Denise K. Gessner Klaus Failing Dieter Lütjohann Klaus Eder Effects of supplementation of DL-methionine on tissue and plasma antioxidant status during heat-induced oxidative stress in broilers Poultry Science methionine heat stress oxidative stress antioxidant response broiler |
| title | Effects of supplementation of DL-methionine on tissue and plasma antioxidant status during heat-induced oxidative stress in broilers |
| title_full | Effects of supplementation of DL-methionine on tissue and plasma antioxidant status during heat-induced oxidative stress in broilers |
| title_fullStr | Effects of supplementation of DL-methionine on tissue and plasma antioxidant status during heat-induced oxidative stress in broilers |
| title_full_unstemmed | Effects of supplementation of DL-methionine on tissue and plasma antioxidant status during heat-induced oxidative stress in broilers |
| title_short | Effects of supplementation of DL-methionine on tissue and plasma antioxidant status during heat-induced oxidative stress in broilers |
| title_sort | effects of supplementation of dl methionine on tissue and plasma antioxidant status during heat induced oxidative stress in broilers |
| topic | methionine heat stress oxidative stress antioxidant response broiler |
| url | http://www.sciencedirect.com/science/article/pii/S0032579120306295 |
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