Relationships between lipolysis induced by various lipolytic agents and hormone-sensitive lipase in rat fat cells
Norepinephrine induced lipolysis in rat fat cells, in vitro, in a time- and concentration-dependent manner, without concomitantly increasing hormone-sensitive lipase (HSL) activity. It also induced, time and concentration dependently, HSL translocation from the cytosol to the lipid droplets in fat c...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2001-01-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520323439 |
| _version_ | 1818676067237363712 |
|---|---|
| author | Chie Morimoto Kenji Kameda Takahiro Tsujita Hiromichi Okuda |
| author_facet | Chie Morimoto Kenji Kameda Takahiro Tsujita Hiromichi Okuda |
| author_sort | Chie Morimoto |
| collection | DOAJ |
| description | Norepinephrine induced lipolysis in rat fat cells, in vitro, in a time- and concentration-dependent manner, without concomitantly increasing hormone-sensitive lipase (HSL) activity. It also induced, time and concentration dependently, HSL translocation from the cytosol to the lipid droplets in fat cells. Isoproterenol, forskolin, dibutyryl cyclic AMP, and theophylline also induced lipolysis in fat cells, but did not stimulate HSL activity. These agents also induced HSL translocation from the cytosol to the lipid droplets in fat cells: about 80% to 90% of all HSL was located in lipid droplets after incubation for 1 h. These results suggest that the critical event in lipolytic activation of fat cells induced by lipolytic agents is not an increase in the catalytic activity of HSL but translocation of HSL to its substrate on the surfaces of lipid droplets in fat cells.—Morimoto, C., K. Kameda, T. Tsujita, and H. Okuda. Relationships between lipolysis induced by various lipolytic agents and hormone-sensitive lipase in rat fat cells. J. Lipid Res. 2001. 42: 120–127. |
| first_indexed | 2024-12-17T08:37:35Z |
| format | Article |
| id | doaj.art-33ea05e939a14fe7aa9b71ea4a73faaf |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T08:37:35Z |
| publishDate | 2001-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-33ea05e939a14fe7aa9b71ea4a73faaf2022-12-21T21:56:26ZengElsevierJournal of Lipid Research0022-22752001-01-01421120127Relationships between lipolysis induced by various lipolytic agents and hormone-sensitive lipase in rat fat cellsChie Morimoto0Kenji Kameda1Takahiro Tsujita2Hiromichi Okuda3Second Department of Medical Biochemistry, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-0295, Japan; Department of Chemistry, Faculty of Science, Ehime University, Matsuyama, Ehime 790-8577, JapanCentral Research Laboratory, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-0295, JapanCentral Research Laboratory, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-0295, JapanSecond Department of Medical Biochemistry, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-0295, JapanNorepinephrine induced lipolysis in rat fat cells, in vitro, in a time- and concentration-dependent manner, without concomitantly increasing hormone-sensitive lipase (HSL) activity. It also induced, time and concentration dependently, HSL translocation from the cytosol to the lipid droplets in fat cells. Isoproterenol, forskolin, dibutyryl cyclic AMP, and theophylline also induced lipolysis in fat cells, but did not stimulate HSL activity. These agents also induced HSL translocation from the cytosol to the lipid droplets in fat cells: about 80% to 90% of all HSL was located in lipid droplets after incubation for 1 h. These results suggest that the critical event in lipolytic activation of fat cells induced by lipolytic agents is not an increase in the catalytic activity of HSL but translocation of HSL to its substrate on the surfaces of lipid droplets in fat cells.—Morimoto, C., K. Kameda, T. Tsujita, and H. Okuda. Relationships between lipolysis induced by various lipolytic agents and hormone-sensitive lipase in rat fat cells. J. Lipid Res. 2001. 42: 120–127.http://www.sciencedirect.com/science/article/pii/S0022227520323439HSL acivityHSL translocationendogenous lipid droplet |
| spellingShingle | Chie Morimoto Kenji Kameda Takahiro Tsujita Hiromichi Okuda Relationships between lipolysis induced by various lipolytic agents and hormone-sensitive lipase in rat fat cells Journal of Lipid Research HSL acivity HSL translocation endogenous lipid droplet |
| title | Relationships between lipolysis induced by various lipolytic agents and hormone-sensitive lipase in rat fat cells |
| title_full | Relationships between lipolysis induced by various lipolytic agents and hormone-sensitive lipase in rat fat cells |
| title_fullStr | Relationships between lipolysis induced by various lipolytic agents and hormone-sensitive lipase in rat fat cells |
| title_full_unstemmed | Relationships between lipolysis induced by various lipolytic agents and hormone-sensitive lipase in rat fat cells |
| title_short | Relationships between lipolysis induced by various lipolytic agents and hormone-sensitive lipase in rat fat cells |
| title_sort | relationships between lipolysis induced by various lipolytic agents and hormone sensitive lipase in rat fat cells |
| topic | HSL acivity HSL translocation endogenous lipid droplet |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520323439 |
| work_keys_str_mv | AT chiemorimoto relationshipsbetweenlipolysisinducedbyvariouslipolyticagentsandhormonesensitivelipaseinratfatcells AT kenjikameda relationshipsbetweenlipolysisinducedbyvariouslipolyticagentsandhormonesensitivelipaseinratfatcells AT takahirotsujita relationshipsbetweenlipolysisinducedbyvariouslipolyticagentsandhormonesensitivelipaseinratfatcells AT hiromichiokuda relationshipsbetweenlipolysisinducedbyvariouslipolyticagentsandhormonesensitivelipaseinratfatcells |