Search for SARS-CoV-2 RNA in platelets from COVID-19 patients
The frequent finding of thrombocytopenia in patients with severe SARS-CoV-2 infection (COVID-19) and previous evidence that several viruses enter platelets suggest that SARS-CoV-2 might be internalized by platelets of COVID-19. Aim of our study was to assess the presence of SARS-CoV-2 RNA in platele...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-02-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2020.1859104 |
| _version_ | 1827816736501530624 |
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| author | Loredana Bury Barbara Camilloni Roberto Castronari Elisa Piselli Marco Malvestiti Mariachiara Borghi Haripriya KuchiBotla Emanuela Falcinelli Eleonora Petito Felice Amato Ugo Paliani Gaetano Vaudo Vittorio Cerotto Fabio Gori Cecilia Becattini Edoardo De Robertis Teseo Lazzarini Giuseppe Castaldo Antonella Mencacci Paolo Gresele |
| author_facet | Loredana Bury Barbara Camilloni Roberto Castronari Elisa Piselli Marco Malvestiti Mariachiara Borghi Haripriya KuchiBotla Emanuela Falcinelli Eleonora Petito Felice Amato Ugo Paliani Gaetano Vaudo Vittorio Cerotto Fabio Gori Cecilia Becattini Edoardo De Robertis Teseo Lazzarini Giuseppe Castaldo Antonella Mencacci Paolo Gresele |
| author_sort | Loredana Bury |
| collection | DOAJ |
| description | The frequent finding of thrombocytopenia in patients with severe SARS-CoV-2 infection (COVID-19) and previous evidence that several viruses enter platelets suggest that SARS-CoV-2 might be internalized by platelets of COVID-19. Aim of our study was to assess the presence of SARS-CoV-2 RNA in platelets from hospitalized patients with aconfirmed diagnosis of COVID-19. RNA was extracted from platelets, leukocytes and serum from 24 COVID-19 patients and 3 healthy controls, real-time PCR and ddPCR for viral genes were carried out. SARS-CoV-2 RNA was not detected in any of the samples analyzed nor in healthy controls, by either RT-PCR or ddPCR, while RNA samples from nasopharyngeal swabs of COVID-19 patients were correctly identified. Viral RNA was not detected independently of viral load, of positive nasopharyngeal swabs, or viremia, the last detected in only one patient (4.1%). SARS-CoV-2 entry in platelets is not acommon phenomenon in COVID-19 patients, differently from other viral infections. |
| first_indexed | 2024-03-12T00:26:02Z |
| format | Article |
| id | doaj.art-33ea8429f11942a3870128e00e97ee27 |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:26:02Z |
| publishDate | 2021-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-33ea8429f11942a3870128e00e97ee272023-09-15T10:38:09ZengTaylor & Francis GroupPlatelets0953-71041369-16352021-02-0132228428710.1080/09537104.2020.18591041859104Search for SARS-CoV-2 RNA in platelets from COVID-19 patientsLoredana Bury0Barbara Camilloni1Roberto Castronari2Elisa Piselli3Marco Malvestiti4Mariachiara Borghi5Haripriya KuchiBotla6Emanuela Falcinelli7Eleonora Petito8Felice Amato9Ugo Paliani10Gaetano Vaudo11Vittorio Cerotto12Fabio Gori13Cecilia Becattini14Edoardo De Robertis15Teseo Lazzarini16Giuseppe Castaldo17Antonella Mencacci18Paolo Gresele19University of PerugiaUniversity of PerugiaUniversity of PerugiaUniversity of PerugiaUniversity of PerugiaUniversity of PerugiaUniversity of PerugiaUniversity of PerugiaUniversity of PerugiaUniversità Di Napoli Federico IIASL 1 UmbriaTerni University HospitalCittà Di Castello HospitalAzienda Ospedaliera-Universitaria Santa Maria Della MisericordiaUniversity of PerugiaUniversity of PerugiaPresidio Alto Chiascio, USL Umbria 1Università Di Napoli Federico IIUniversity of PerugiaUniversity of PerugiaThe frequent finding of thrombocytopenia in patients with severe SARS-CoV-2 infection (COVID-19) and previous evidence that several viruses enter platelets suggest that SARS-CoV-2 might be internalized by platelets of COVID-19. Aim of our study was to assess the presence of SARS-CoV-2 RNA in platelets from hospitalized patients with aconfirmed diagnosis of COVID-19. RNA was extracted from platelets, leukocytes and serum from 24 COVID-19 patients and 3 healthy controls, real-time PCR and ddPCR for viral genes were carried out. SARS-CoV-2 RNA was not detected in any of the samples analyzed nor in healthy controls, by either RT-PCR or ddPCR, while RNA samples from nasopharyngeal swabs of COVID-19 patients were correctly identified. Viral RNA was not detected independently of viral load, of positive nasopharyngeal swabs, or viremia, the last detected in only one patient (4.1%). SARS-CoV-2 entry in platelets is not acommon phenomenon in COVID-19 patients, differently from other viral infections.http://dx.doi.org/10.1080/09537104.2020.1859104covid-19platelet rnasars-cov-2viral infection |
| spellingShingle | Loredana Bury Barbara Camilloni Roberto Castronari Elisa Piselli Marco Malvestiti Mariachiara Borghi Haripriya KuchiBotla Emanuela Falcinelli Eleonora Petito Felice Amato Ugo Paliani Gaetano Vaudo Vittorio Cerotto Fabio Gori Cecilia Becattini Edoardo De Robertis Teseo Lazzarini Giuseppe Castaldo Antonella Mencacci Paolo Gresele Search for SARS-CoV-2 RNA in platelets from COVID-19 patients Platelets covid-19 platelet rna sars-cov-2 viral infection |
| title | Search for SARS-CoV-2 RNA in platelets from COVID-19 patients |
| title_full | Search for SARS-CoV-2 RNA in platelets from COVID-19 patients |
| title_fullStr | Search for SARS-CoV-2 RNA in platelets from COVID-19 patients |
| title_full_unstemmed | Search for SARS-CoV-2 RNA in platelets from COVID-19 patients |
| title_short | Search for SARS-CoV-2 RNA in platelets from COVID-19 patients |
| title_sort | search for sars cov 2 rna in platelets from covid 19 patients |
| topic | covid-19 platelet rna sars-cov-2 viral infection |
| url | http://dx.doi.org/10.1080/09537104.2020.1859104 |
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