A serological survey of canine leptospirosis in the city of Belgrade, Serbia
Canine leptospirosis is a zoonosis caused by bacteria belonging to the genus Leptospira. Dogs are one of the animal species involved in the cycle of preservation and transmission of leptospirosis in urban areas. Serological testing for the presence of specific antibodies against Leptospira spp. in d...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Belgrade
2022-01-01
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| Series: | Veterinarski Glasnik |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0350-2457/2022/0350-24572200001V.pdf |
| _version_ | 1818049009054384128 |
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| author | Vojinović Dragica Žutić Jadranka Vasić Ana Stanojević Slobodan Spalević Ljiljana Zurovac-Sapundžić Zorana |
| author_facet | Vojinović Dragica Žutić Jadranka Vasić Ana Stanojević Slobodan Spalević Ljiljana Zurovac-Sapundžić Zorana |
| author_sort | Vojinović Dragica |
| collection | DOAJ |
| description | Canine leptospirosis is a zoonosis caused by bacteria belonging to the genus Leptospira. Dogs are one of the animal species involved in the cycle of preservation and transmission of leptospirosis in urban areas. Serological testing for the presence of specific antibodies against Leptospira spp. in dogs was continuously performed between 2010 and 2020 in the city of Belgrade. At the request of the owners themselves, other veterinary laboratories or laboratory clinics, 179 blood sera from 179 dogs were examined in the Laboratory for Immunology, Scientific Institute of Veterinary Medicine of Serbia. Blood sera samples from dogs were examined using the standard microscopic agglutination test (MAT) for the presence of specific antibodies against seven different serovars of Leptospira: Pomona, Icterohaemorrhagiae, Grippotyphosa, Sejroe, Canicola, Bataviae, and Australis. The number of seropositive dogs was 17/179 (9.5%). Among all examined sera, the highest titre of seropositive samples was to serovar Icterohaemorrhagiae (10/17, 58.8%), followed by Pomona (4/17, 23.5%), and serovar Canicola (3/17, 17.6%). Specific antibodies for serovars Grippotyphosa, Sejroe, Bataviae and Australis were not detected in any of the dog sera. Cross-reaction (the presence of two or three titres with different values where one of them was higher than others) between different serovars was diagnosed in a low number of sera (n=4), with the following serovars: Icterohaemorrhagiae and Pomona (n=3) and Pomona and Canicola (n=1). The confirmed specific antibody titres for Leptospira spp. were between 1:100 to 1:3000 (5 sera had titres of 1:100, 7 had titres of 1:300, 4 had titres of 1:1000, and 1 serum had a titre 1:3000). Monitoring canine leptospirosis is a useful tool in preventing leptospirosis in Belgrade. |
| first_indexed | 2024-12-10T10:30:45Z |
| format | Article |
| id | doaj.art-33eb670241314bbdba89d936ae6ff40b |
| institution | Directory Open Access Journal |
| issn | 0350-2457 2406-0771 |
| language | English |
| last_indexed | 2024-12-10T10:30:45Z |
| publishDate | 2022-01-01 |
| publisher | Faculty of Veterinary Medicine, Belgrade |
| record_format | Article |
| series | Veterinarski Glasnik |
| spelling | doaj.art-33eb670241314bbdba89d936ae6ff40b2022-12-22T01:52:34ZengFaculty of Veterinary Medicine, BelgradeVeterinarski Glasnik0350-24572406-07712022-01-01761475510.2298/VETGL210708001V0350-24572200001VA serological survey of canine leptospirosis in the city of Belgrade, SerbiaVojinović Dragica0https://orcid.org/0000-0002-4890-3239Žutić Jadranka1https://orcid.org/0000-0003-2207-2040Vasić Ana2https://orcid.org/0000-0002-0653-2935Stanojević Slobodan3Spalević Ljiljana4Zurovac-Sapundžić Zorana5https://orcid.org/0000-0003-2935-9554Scientific Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaScientific Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaInstitute for Medical Research, National Institute of Serbia, University of Belgrade, Belgrade, SerbiaScientific Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaScientific Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaScientific Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaCanine leptospirosis is a zoonosis caused by bacteria belonging to the genus Leptospira. Dogs are one of the animal species involved in the cycle of preservation and transmission of leptospirosis in urban areas. Serological testing for the presence of specific antibodies against Leptospira spp. in dogs was continuously performed between 2010 and 2020 in the city of Belgrade. At the request of the owners themselves, other veterinary laboratories or laboratory clinics, 179 blood sera from 179 dogs were examined in the Laboratory for Immunology, Scientific Institute of Veterinary Medicine of Serbia. Blood sera samples from dogs were examined using the standard microscopic agglutination test (MAT) for the presence of specific antibodies against seven different serovars of Leptospira: Pomona, Icterohaemorrhagiae, Grippotyphosa, Sejroe, Canicola, Bataviae, and Australis. The number of seropositive dogs was 17/179 (9.5%). Among all examined sera, the highest titre of seropositive samples was to serovar Icterohaemorrhagiae (10/17, 58.8%), followed by Pomona (4/17, 23.5%), and serovar Canicola (3/17, 17.6%). Specific antibodies for serovars Grippotyphosa, Sejroe, Bataviae and Australis were not detected in any of the dog sera. Cross-reaction (the presence of two or three titres with different values where one of them was higher than others) between different serovars was diagnosed in a low number of sera (n=4), with the following serovars: Icterohaemorrhagiae and Pomona (n=3) and Pomona and Canicola (n=1). The confirmed specific antibody titres for Leptospira spp. were between 1:100 to 1:3000 (5 sera had titres of 1:100, 7 had titres of 1:300, 4 had titres of 1:1000, and 1 serum had a titre 1:3000). Monitoring canine leptospirosis is a useful tool in preventing leptospirosis in Belgrade.http://www.doiserbia.nb.rs/img/doi/0350-2457/2022/0350-24572200001V.pdfbelgradedogsleptospira spp.mat |
| spellingShingle | Vojinović Dragica Žutić Jadranka Vasić Ana Stanojević Slobodan Spalević Ljiljana Zurovac-Sapundžić Zorana A serological survey of canine leptospirosis in the city of Belgrade, Serbia Veterinarski Glasnik belgrade dogs leptospira spp. mat |
| title | A serological survey of canine leptospirosis in the city of Belgrade, Serbia |
| title_full | A serological survey of canine leptospirosis in the city of Belgrade, Serbia |
| title_fullStr | A serological survey of canine leptospirosis in the city of Belgrade, Serbia |
| title_full_unstemmed | A serological survey of canine leptospirosis in the city of Belgrade, Serbia |
| title_short | A serological survey of canine leptospirosis in the city of Belgrade, Serbia |
| title_sort | serological survey of canine leptospirosis in the city of belgrade serbia |
| topic | belgrade dogs leptospira spp. mat |
| url | http://www.doiserbia.nb.rs/img/doi/0350-2457/2022/0350-24572200001V.pdf |
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