Development of lumen-based perfusable 3D liver in vitro model using single-step bioprinting with composite bioinks
Hepatic sinusoids are uniquely organized structures that help maintain a spectrum of hepatic functions. Although several in vitro liver models have been developed to replicate liver sinusoids, most of these platforms require complex, multi-step fabrication methods making it difficult to achieve trul...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423001837 |
| _version_ | 1797770725891768320 |
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| author | Somnath Maji Minkyoung Lee Jooyoung Lee Jaehee Lee Hyungseok Lee |
| author_facet | Somnath Maji Minkyoung Lee Jooyoung Lee Jaehee Lee Hyungseok Lee |
| author_sort | Somnath Maji |
| collection | DOAJ |
| description | Hepatic sinusoids are uniquely organized structures that help maintain a spectrum of hepatic functions. Although several in vitro liver models have been developed to replicate liver sinusoids, most of these platforms require complex, multi-step fabrication methods making it difficult to achieve truly three-dimensional (3D) channel geometries. In this study, a single-step bioprinting technique was demonstrated to simultaneously print a chip platform and develop a perfusable vascularized liver sinusoid in vitro model. The integrated system uses a co-axial-based bioprinting approach to develop a liver sinusoid-like model that consists of a sacrificial core compartment containing a perfusable pre-vascular structure and an alginate-collagen-based shell compartment containing hepatocytes. The lumen-based perfusable 3D liver sinusoid-on-a-chip (LSOC-P) demonstrated significantly better hepatocyte viability, proliferation, and liver-specific gene and protein expression compared to a 3D hepatocyte-based core/shell model with static media and the standard hepatocyte-based 2D sandwich culture system. A drug toxicity evaluation of hepatotoxins highlighted the comparatively higher sensitivity of the LSOC system with a close estimation of the therapeutic range of safe drug concentrations for humans. In conclusion, the current findings indicate that the combinatorial single-step co-axial bioprinting technique is a promising fabrication approach for the development of a perfusable LSOC platform for drug screening applications. |
| first_indexed | 2024-03-12T21:27:23Z |
| format | Article |
| id | doaj.art-33ed8bf642444549ab666c5621d3c089 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-12T21:27:23Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-33ed8bf642444549ab666c5621d3c0892023-07-28T04:26:45ZengElsevierMaterials Today Bio2590-00642023-08-0121100723Development of lumen-based perfusable 3D liver in vitro model using single-step bioprinting with composite bioinksSomnath Maji0Minkyoung Lee1Jooyoung Lee2Jaehee Lee3Hyungseok Lee4Department of Mechanical and Biomedical Engineering, Kangwon National University, Chuncheon, Republic of KoreaDepartment of Animal Industry Convergence, Kangwon National University, Chuncheon, Republic of Korea; Department of Smart Health Science and Technology, Kangwon National University, Chuncheon, Republic of KoreaDepartment of Smart Health Science and Technology, Kangwon National University, Chuncheon, Republic of KoreaDepartment of Smart Health Science and Technology, Kangwon National University, Chuncheon, Republic of KoreaDepartment of Mechanical and Biomedical Engineering, Kangwon National University, Chuncheon, Republic of Korea; Department of Smart Health Science and Technology, Kangwon National University, Chuncheon, Republic of Korea; Corresponding author. Department of Mechanical and Biomedical Engineering, Kangwon National University (KNU), 1, Kangwondaehak-gil, Chuncheon-si, Gangwon-do, Republic of Korea.Hepatic sinusoids are uniquely organized structures that help maintain a spectrum of hepatic functions. Although several in vitro liver models have been developed to replicate liver sinusoids, most of these platforms require complex, multi-step fabrication methods making it difficult to achieve truly three-dimensional (3D) channel geometries. In this study, a single-step bioprinting technique was demonstrated to simultaneously print a chip platform and develop a perfusable vascularized liver sinusoid in vitro model. The integrated system uses a co-axial-based bioprinting approach to develop a liver sinusoid-like model that consists of a sacrificial core compartment containing a perfusable pre-vascular structure and an alginate-collagen-based shell compartment containing hepatocytes. The lumen-based perfusable 3D liver sinusoid-on-a-chip (LSOC-P) demonstrated significantly better hepatocyte viability, proliferation, and liver-specific gene and protein expression compared to a 3D hepatocyte-based core/shell model with static media and the standard hepatocyte-based 2D sandwich culture system. A drug toxicity evaluation of hepatotoxins highlighted the comparatively higher sensitivity of the LSOC system with a close estimation of the therapeutic range of safe drug concentrations for humans. In conclusion, the current findings indicate that the combinatorial single-step co-axial bioprinting technique is a promising fabrication approach for the development of a perfusable LSOC platform for drug screening applications.http://www.sciencedirect.com/science/article/pii/S2590006423001837Liver-on-a-chipBioprintingSingle-step fabricationLiver sinusoidDrug screening |
| spellingShingle | Somnath Maji Minkyoung Lee Jooyoung Lee Jaehee Lee Hyungseok Lee Development of lumen-based perfusable 3D liver in vitro model using single-step bioprinting with composite bioinks Materials Today Bio Liver-on-a-chip Bioprinting Single-step fabrication Liver sinusoid Drug screening |
| title | Development of lumen-based perfusable 3D liver in vitro model using single-step bioprinting with composite bioinks |
| title_full | Development of lumen-based perfusable 3D liver in vitro model using single-step bioprinting with composite bioinks |
| title_fullStr | Development of lumen-based perfusable 3D liver in vitro model using single-step bioprinting with composite bioinks |
| title_full_unstemmed | Development of lumen-based perfusable 3D liver in vitro model using single-step bioprinting with composite bioinks |
| title_short | Development of lumen-based perfusable 3D liver in vitro model using single-step bioprinting with composite bioinks |
| title_sort | development of lumen based perfusable 3d liver in vitro model using single step bioprinting with composite bioinks |
| topic | Liver-on-a-chip Bioprinting Single-step fabrication Liver sinusoid Drug screening |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423001837 |
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