Characterisation of the RNA Virome of Nine <i>Ochlerotatus</i> Species in Finland
RNA viromes of nine commonly encountered <i>Ochlerotatus</i> mosquito species collected around Finland in 2015 and 2017 were studied using next-generation sequencing. Mosquito homogenates were sequenced from 91 pools comprising 16–60 morphologically identified adult females of <i>O...
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MDPI AG
2022-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/7/1489 |
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| author | Phuoc T. Truong Nguyen C. Lorna Culverwell Maija T. Suvanto Essi M. Korhonen Ruut Uusitalo Olli Vapalahti Teemu Smura Eili Huhtamo |
| author_facet | Phuoc T. Truong Nguyen C. Lorna Culverwell Maija T. Suvanto Essi M. Korhonen Ruut Uusitalo Olli Vapalahti Teemu Smura Eili Huhtamo |
| author_sort | Phuoc T. Truong Nguyen |
| collection | DOAJ |
| description | RNA viromes of nine commonly encountered <i>Ochlerotatus</i> mosquito species collected around Finland in 2015 and 2017 were studied using next-generation sequencing. Mosquito homogenates were sequenced from 91 pools comprising 16–60 morphologically identified adult females of <i>Oc. cantans</i>, <i>Oc. caspius</i>, <i>Oc. communis</i>, <i>Oc. diantaeus</i>, <i>Oc. excrucians</i>, <i>Oc. hexodontus</i>, <i>Oc. intrudens</i>, <i>Oc. pullatus</i> and <i>Oc. punctor</i>/<i>punctodes</i>. In total 514 viral Reverse dependent RNA polymerase (RdRp) sequences of 159 virus species were recovered, belonging to 25 families or equivalent rank, as follows: <i>Aliusviridae</i>, <i>Aspiviridae</i>, <i>Botybirnavirus</i>, <i>Chrysoviridae</i>, <i>Chuviridae</i>, <i>Endornaviridae</i>, <i>Flaviviridae</i>, <i>Iflaviridae</i>, <i>Negevirus</i>, <i>Partitiviridae</i>, <i>Permutotetraviridae</i>, <i>Phasmaviridae</i>, <i>Phenuiviridae</i>, <i>Picornaviridae</i>, <i>Qinviridae</i>, <i>Quenyavirus</i>, <i>Rhabdoviridae</i>, <i>Sedoreoviridae</i>, <i>Solemoviridae</i>, <i>Spinareoviridae</i>, <i>Togaviridae</i>, <i>Totiviridae</i>, <i>Virgaviridae</i>, <i>Xinmoviridae</i> and <i>Yueviridae</i>. Of these, 147 are tentatively novel viruses. One sequence of <i>Sindbis virus</i>, which causes Pogosta disease in humans, was detected from <i>Oc. communis</i> from Pohjois-Karjala. This study greatly increases the number of mosquito-associated viruses known from Finland and presents the northern-most mosquito-associated viruses in Europe to date. |
| first_indexed | 2024-03-09T05:42:00Z |
| format | Article |
| id | doaj.art-33edef97adca4dbdaa7c5867d93948ae |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T05:42:00Z |
| publishDate | 2022-07-01 |
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| series | Viruses |
| spelling | doaj.art-33edef97adca4dbdaa7c5867d93948ae2023-12-03T12:24:12ZengMDPI AGViruses1999-49152022-07-01147148910.3390/v14071489Characterisation of the RNA Virome of Nine <i>Ochlerotatus</i> Species in FinlandPhuoc T. Truong Nguyen0C. Lorna Culverwell1Maija T. Suvanto2Essi M. Korhonen3Ruut Uusitalo4Olli Vapalahti5Teemu Smura6Eili Huhtamo7Department of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, FinlandDepartment of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, FinlandDepartment of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, FinlandDepartment of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, FinlandDepartment of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, FinlandDepartment of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, FinlandDepartment of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, FinlandDepartment of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, FinlandRNA viromes of nine commonly encountered <i>Ochlerotatus</i> mosquito species collected around Finland in 2015 and 2017 were studied using next-generation sequencing. Mosquito homogenates were sequenced from 91 pools comprising 16–60 morphologically identified adult females of <i>Oc. cantans</i>, <i>Oc. caspius</i>, <i>Oc. communis</i>, <i>Oc. diantaeus</i>, <i>Oc. excrucians</i>, <i>Oc. hexodontus</i>, <i>Oc. intrudens</i>, <i>Oc. pullatus</i> and <i>Oc. punctor</i>/<i>punctodes</i>. In total 514 viral Reverse dependent RNA polymerase (RdRp) sequences of 159 virus species were recovered, belonging to 25 families or equivalent rank, as follows: <i>Aliusviridae</i>, <i>Aspiviridae</i>, <i>Botybirnavirus</i>, <i>Chrysoviridae</i>, <i>Chuviridae</i>, <i>Endornaviridae</i>, <i>Flaviviridae</i>, <i>Iflaviridae</i>, <i>Negevirus</i>, <i>Partitiviridae</i>, <i>Permutotetraviridae</i>, <i>Phasmaviridae</i>, <i>Phenuiviridae</i>, <i>Picornaviridae</i>, <i>Qinviridae</i>, <i>Quenyavirus</i>, <i>Rhabdoviridae</i>, <i>Sedoreoviridae</i>, <i>Solemoviridae</i>, <i>Spinareoviridae</i>, <i>Togaviridae</i>, <i>Totiviridae</i>, <i>Virgaviridae</i>, <i>Xinmoviridae</i> and <i>Yueviridae</i>. Of these, 147 are tentatively novel viruses. One sequence of <i>Sindbis virus</i>, which causes Pogosta disease in humans, was detected from <i>Oc. communis</i> from Pohjois-Karjala. This study greatly increases the number of mosquito-associated viruses known from Finland and presents the northern-most mosquito-associated viruses in Europe to date.https://www.mdpi.com/1999-4915/14/7/1489Aedinimosquito virome<i>Ochlerotatus</i>NGSFinlandRNA virus |
| spellingShingle | Phuoc T. Truong Nguyen C. Lorna Culverwell Maija T. Suvanto Essi M. Korhonen Ruut Uusitalo Olli Vapalahti Teemu Smura Eili Huhtamo Characterisation of the RNA Virome of Nine <i>Ochlerotatus</i> Species in Finland Viruses Aedini mosquito virome <i>Ochlerotatus</i> NGS Finland RNA virus |
| title | Characterisation of the RNA Virome of Nine <i>Ochlerotatus</i> Species in Finland |
| title_full | Characterisation of the RNA Virome of Nine <i>Ochlerotatus</i> Species in Finland |
| title_fullStr | Characterisation of the RNA Virome of Nine <i>Ochlerotatus</i> Species in Finland |
| title_full_unstemmed | Characterisation of the RNA Virome of Nine <i>Ochlerotatus</i> Species in Finland |
| title_short | Characterisation of the RNA Virome of Nine <i>Ochlerotatus</i> Species in Finland |
| title_sort | characterisation of the rna virome of nine i ochlerotatus i species in finland |
| topic | Aedini mosquito virome <i>Ochlerotatus</i> NGS Finland RNA virus |
| url | https://www.mdpi.com/1999-4915/14/7/1489 |
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