Host-Pathogen Interactions of <i>Mycoplasma mycoides</i> in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models
Respiratory infections caused by mycoplasma species in ruminants lead to considerable economic losses. Two important ruminant pathogens are <i>Mycoplasma mycoides</i> subsp. <i>Mycoides</i> (<i>Mmm</i>), the aetiological agent of contagious bovine pleuropneumonia...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/8/2/82 |
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| author | Yenehiwot Berhanu Weldearegay Sandy Müller Jana Hänske Anja Schulze Aline Kostka Nancy Rüger Marion Hewicker-Trautwein Ralph Brehm Peter Valentin-Weigand Robert Kammerer Joerg Jores Jochen Meens |
| author_facet | Yenehiwot Berhanu Weldearegay Sandy Müller Jana Hänske Anja Schulze Aline Kostka Nancy Rüger Marion Hewicker-Trautwein Ralph Brehm Peter Valentin-Weigand Robert Kammerer Joerg Jores Jochen Meens |
| author_sort | Yenehiwot Berhanu Weldearegay |
| collection | DOAJ |
| description | Respiratory infections caused by mycoplasma species in ruminants lead to considerable economic losses. Two important ruminant pathogens are <i>Mycoplasma mycoides</i> subsp. <i>Mycoides</i> (<i>Mmm</i>), the aetiological agent of contagious bovine pleuropneumonia and <i>Mycoplasma mycoides</i> subsp. <i>capri</i> (<i>Mmc</i>), which causes pneumonia, mastitis, arthritis, keratitis, and septicemia in goats. We established precision cut lung slices (PCLS) infection model for <i>Mmm</i> and <i>Mmc</i> to study host-pathogen interactions. We monitored infection over time using immunohistological analysis and electron microscopy. Moreover, infection burden was monitored by plating and quantitative real-time PCR. Results were compared with lungs from experimentally infected goats and cattle. Lungs from healthy goats and cattle were also included as controls. PCLS remained viable for up to two weeks. Both subspecies adhered to ciliated cells. However, the titer of <i>Mmm</i> in caprine PCLS decreased over time, indicating species specificity of <i>Mmm</i>. <i>Mmc</i> showed higher tropism to sub-bronchiolar tissue in caprine PCLS, which increased in a time-dependent manner. Moreover, <i>Mmc</i> was abundantly observed on pulmonary endothelial cells, indicating partially, how it causes systemic disease. Tissue destruction upon prolonged infection of slices was comparable to the in vivo samples. Therefore, PCLS represents a novel ex vivo model to study host-pathogen interaction in livestock mycoplasma. |
| first_indexed | 2024-04-13T08:26:36Z |
| format | Article |
| id | doaj.art-33f00230ff084278b48503a12f0e2b9d |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-13T08:26:36Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-33f00230ff084278b48503a12f0e2b9d2022-12-22T02:54:26ZengMDPI AGPathogens2076-08172019-06-01828210.3390/pathogens8020082pathogens8020082Host-Pathogen Interactions of <i>Mycoplasma mycoides</i> in Caprine and Bovine Precision-Cut Lung Slices (PCLS) ModelsYenehiwot Berhanu Weldearegay0Sandy Müller1Jana Hänske2Anja Schulze3Aline Kostka4Nancy Rüger5Marion Hewicker-Trautwein6Ralph Brehm7Peter Valentin-Weigand8Robert Kammerer9Joerg Jores10Jochen Meens11Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, 30173 Hannover, GermanyInstitute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, 30173 Hannover, GermanyInstitute of Immunology, Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, GermanyInstitute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, 30173 Hannover, GermanyInstitute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, 30173 Hannover, GermanyInstitute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, 30173 Hannover, GermanyInstitute for Pathology, University of Veterinary Medicine Hannover, 30559 Hannover, GermanyInstitute of Anatomy, University of Veterinary Medicine Hannover, 30173 Hannover, GermanyInstitute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, 30173 Hannover, GermanyInstitute of Immunology, Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, GermanyInstitute of Veterinary Bacteriology, University of Bern, CH-3001 Bern, SwitzerlandInstitute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, 30173 Hannover, GermanyRespiratory infections caused by mycoplasma species in ruminants lead to considerable economic losses. Two important ruminant pathogens are <i>Mycoplasma mycoides</i> subsp. <i>Mycoides</i> (<i>Mmm</i>), the aetiological agent of contagious bovine pleuropneumonia and <i>Mycoplasma mycoides</i> subsp. <i>capri</i> (<i>Mmc</i>), which causes pneumonia, mastitis, arthritis, keratitis, and septicemia in goats. We established precision cut lung slices (PCLS) infection model for <i>Mmm</i> and <i>Mmc</i> to study host-pathogen interactions. We monitored infection over time using immunohistological analysis and electron microscopy. Moreover, infection burden was monitored by plating and quantitative real-time PCR. Results were compared with lungs from experimentally infected goats and cattle. Lungs from healthy goats and cattle were also included as controls. PCLS remained viable for up to two weeks. Both subspecies adhered to ciliated cells. However, the titer of <i>Mmm</i> in caprine PCLS decreased over time, indicating species specificity of <i>Mmm</i>. <i>Mmc</i> showed higher tropism to sub-bronchiolar tissue in caprine PCLS, which increased in a time-dependent manner. Moreover, <i>Mmc</i> was abundantly observed on pulmonary endothelial cells, indicating partially, how it causes systemic disease. Tissue destruction upon prolonged infection of slices was comparable to the in vivo samples. Therefore, PCLS represents a novel ex vivo model to study host-pathogen interaction in livestock mycoplasma.https://www.mdpi.com/2076-0817/8/2/82<i>Mycoplasma mycoides</i>CBPPMAKePS syndromePCLStropismimmunofluorescence3R |
| spellingShingle | Yenehiwot Berhanu Weldearegay Sandy Müller Jana Hänske Anja Schulze Aline Kostka Nancy Rüger Marion Hewicker-Trautwein Ralph Brehm Peter Valentin-Weigand Robert Kammerer Joerg Jores Jochen Meens Host-Pathogen Interactions of <i>Mycoplasma mycoides</i> in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models Pathogens <i>Mycoplasma mycoides</i> CBPP MAKePS syndrome PCLS tropism immunofluorescence 3R |
| title | Host-Pathogen Interactions of <i>Mycoplasma mycoides</i> in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models |
| title_full | Host-Pathogen Interactions of <i>Mycoplasma mycoides</i> in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models |
| title_fullStr | Host-Pathogen Interactions of <i>Mycoplasma mycoides</i> in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models |
| title_full_unstemmed | Host-Pathogen Interactions of <i>Mycoplasma mycoides</i> in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models |
| title_short | Host-Pathogen Interactions of <i>Mycoplasma mycoides</i> in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models |
| title_sort | host pathogen interactions of i mycoplasma mycoides i in caprine and bovine precision cut lung slices pcls models |
| topic | <i>Mycoplasma mycoides</i> CBPP MAKePS syndrome PCLS tropism immunofluorescence 3R |
| url | https://www.mdpi.com/2076-0817/8/2/82 |
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