Biological and Chemical Characterization of <i>Musa paradisiaca</i> Leachate
There is a growing demand for molecules of natural origin for biocontrol and biostimulation, given the current trend away from synthetic chemical products. Leachates extracted from plantain stems were obtained after biodegradation of the plant material. To characterize the leachate, quantitative det...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/12/10/1326 |
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| author | Isabelle Boulogne Philippe Petit Lucienne Desfontaines Gaëlle Durambur Catherine Deborde Cathleen Mirande-Ney Quentin Arnaudin Carole Plasson Julie Grivotte Christophe Chamot Sophie Bernard Gladys Loranger-Merciris |
| author_facet | Isabelle Boulogne Philippe Petit Lucienne Desfontaines Gaëlle Durambur Catherine Deborde Cathleen Mirande-Ney Quentin Arnaudin Carole Plasson Julie Grivotte Christophe Chamot Sophie Bernard Gladys Loranger-Merciris |
| author_sort | Isabelle Boulogne |
| collection | DOAJ |
| description | There is a growing demand for molecules of natural origin for biocontrol and biostimulation, given the current trend away from synthetic chemical products. Leachates extracted from plantain stems were obtained after biodegradation of the plant material. To characterize the leachate, quantitative determinations of nitrogen, carbon, phosphorus, and cations (K<sup>+</sup>, Ca<sup>2+</sup>, Mg<sup>2+</sup>, Na<sup>+</sup>), Q2/4, Q2/6, and Q4/6 absorbance ratios, and metabolomic analysis were carried out. The potential role of plantain leachates as fungicide, elicitor of plant defense, and/or plant biostimulant was evaluated by agar well diffusion method, phenotypic, molecular, and imaging approaches. The plant extracts induced a slight inhibition of fungal growth of an aggressive strain of <i>Colletotrichum gloeosporioides</i>, which causes anthracnose. Organic compounds such as cinnamic, ellagic, quinic, and fulvic acids and indole alkaloid such as ellipticine, along with some minerals such as potassium, calcium, and phosphorus, may be responsible for the inhibition of fungal growth. In addition, jasmonic, benzoic, and salicylic acids, which are known to play a role in plant defense and as biostimulants in tomato, were detected in leachate extract. Indeed, foliar application of banana leachate induced overexpression of <i>LOXD</i>, <i>PPOD</i>, and <i>Worky70-80</i> genes, which are involved in phenylpropanoid metabolism, jasmonic acid biosynthesis, and salicylic acid metabolism, respectively. Leachate also activated root growth in tomato seedlings. However, the main impact of the leachate was observed on mature plants, where it caused a reduction in leaf area and fresh weight, the remodeling of stem cell wall glycopolymers, and an increase in the expression of proline dehydrogenase. |
| first_indexed | 2024-03-10T21:25:15Z |
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| id | doaj.art-33f1fbd30fe346b8a84f1422b9864de9 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T21:25:15Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj.art-33f1fbd30fe346b8a84f1422b9864de92023-11-19T15:43:44ZengMDPI AGBiology2079-77372023-10-011210132610.3390/biology12101326Biological and Chemical Characterization of <i>Musa paradisiaca</i> LeachateIsabelle Boulogne0Philippe Petit1Lucienne Desfontaines2Gaëlle Durambur3Catherine Deborde4Cathleen Mirande-Ney5Quentin Arnaudin6Carole Plasson7Julie Grivotte8Christophe Chamot9Sophie Bernard10Gladys Loranger-Merciris11Université de Rouen Normandie, Normandie Univ, GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, IRIB, GDR CNRS Chemobiologie, RMT BESTIM, F-76000 Rouen, FranceUniversité des Antilles, UMR ISYEB-MNHN-CNRS-Sorbonne Université-EPHE, UFR Sciences Exactes et Naturelles, Campus de Fouillole, F-97157 Pointe-à-Pitre, Guadeloupe, FranceASTRO Agrosystèmes Tropicaux, INRAE, F-97170 Petit-Bourg, Guadeloupe, FranceUniversité de Rouen Normandie, Normandie Univ, GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, IRIB, GDR CNRS Chemobiologie, RMT BESTIM, F-76000 Rouen, FranceINRAE, PROBE Research Infrastructure, BIBS Facility, F-44300 Nantes, FranceUniversité de Rouen Normandie, Normandie Univ, GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, IRIB, GDR CNRS Chemobiologie, RMT BESTIM, F-76000 Rouen, FranceUniversité de Rouen Normandie, Normandie Univ, GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, IRIB, GDR CNRS Chemobiologie, RMT BESTIM, F-76000 Rouen, FranceUniversité de Rouen Normandie, Normandie Univ, GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, IRIB, GDR CNRS Chemobiologie, RMT BESTIM, F-76000 Rouen, FranceUniversité de Rouen Normandie, Normandie Univ, GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, IRIB, GDR CNRS Chemobiologie, RMT BESTIM, F-76000 Rouen, FranceUniversité de Rouen Normandie, Normandie Univ, INSERM, CNRS, HeRacLeS US 51 UAR 2026, PRIMACEN, F-76000 Rouen, FranceUniversité de Rouen Normandie, Normandie Univ, GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, IRIB, GDR CNRS Chemobiologie, RMT BESTIM, F-76000 Rouen, FranceUniversité des Antilles, UMR ISYEB-MNHN-CNRS-Sorbonne Université-EPHE, UFR Sciences Exactes et Naturelles, Campus de Fouillole, F-97157 Pointe-à-Pitre, Guadeloupe, FranceThere is a growing demand for molecules of natural origin for biocontrol and biostimulation, given the current trend away from synthetic chemical products. Leachates extracted from plantain stems were obtained after biodegradation of the plant material. To characterize the leachate, quantitative determinations of nitrogen, carbon, phosphorus, and cations (K<sup>+</sup>, Ca<sup>2+</sup>, Mg<sup>2+</sup>, Na<sup>+</sup>), Q2/4, Q2/6, and Q4/6 absorbance ratios, and metabolomic analysis were carried out. The potential role of plantain leachates as fungicide, elicitor of plant defense, and/or plant biostimulant was evaluated by agar well diffusion method, phenotypic, molecular, and imaging approaches. The plant extracts induced a slight inhibition of fungal growth of an aggressive strain of <i>Colletotrichum gloeosporioides</i>, which causes anthracnose. Organic compounds such as cinnamic, ellagic, quinic, and fulvic acids and indole alkaloid such as ellipticine, along with some minerals such as potassium, calcium, and phosphorus, may be responsible for the inhibition of fungal growth. In addition, jasmonic, benzoic, and salicylic acids, which are known to play a role in plant defense and as biostimulants in tomato, were detected in leachate extract. Indeed, foliar application of banana leachate induced overexpression of <i>LOXD</i>, <i>PPOD</i>, and <i>Worky70-80</i> genes, which are involved in phenylpropanoid metabolism, jasmonic acid biosynthesis, and salicylic acid metabolism, respectively. Leachate also activated root growth in tomato seedlings. However, the main impact of the leachate was observed on mature plants, where it caused a reduction in leaf area and fresh weight, the remodeling of stem cell wall glycopolymers, and an increase in the expression of proline dehydrogenase.https://www.mdpi.com/2079-7737/12/10/1326fungistatic activityplant elicitorfulvic acidsorganic and inorganic saltsindole alkaloidsPNPP |
| spellingShingle | Isabelle Boulogne Philippe Petit Lucienne Desfontaines Gaëlle Durambur Catherine Deborde Cathleen Mirande-Ney Quentin Arnaudin Carole Plasson Julie Grivotte Christophe Chamot Sophie Bernard Gladys Loranger-Merciris Biological and Chemical Characterization of <i>Musa paradisiaca</i> Leachate Biology fungistatic activity plant elicitor fulvic acids organic and inorganic salts indole alkaloids PNPP |
| title | Biological and Chemical Characterization of <i>Musa paradisiaca</i> Leachate |
| title_full | Biological and Chemical Characterization of <i>Musa paradisiaca</i> Leachate |
| title_fullStr | Biological and Chemical Characterization of <i>Musa paradisiaca</i> Leachate |
| title_full_unstemmed | Biological and Chemical Characterization of <i>Musa paradisiaca</i> Leachate |
| title_short | Biological and Chemical Characterization of <i>Musa paradisiaca</i> Leachate |
| title_sort | biological and chemical characterization of i musa paradisiaca i leachate |
| topic | fungistatic activity plant elicitor fulvic acids organic and inorganic salts indole alkaloids PNPP |
| url | https://www.mdpi.com/2079-7737/12/10/1326 |
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