Interaction of Central Glutamatergic and Histaminergic Systems on Food Intake Regulation in Layer Chickens
This study purposed to discover the connection between the central glutamatergic and histaminergic systems on feeding behavior in layer chickens. In the first experiment, chicks obtained intracerebroventricular (ICV) injections of saline (control solution), α-FMH (250 nmol), glutamate (300 nmol), an...
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| Format: | Article |
| Language: | English |
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Razi Vaccine and Serum Research Institute
2021-09-01
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| Series: | Archives of Razi Institute |
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| Online Access: | https://archrazi.areeo.ac.ir/article_122236_7e323f10ea9e4f2bef48d786f1d71fd5.pdf |
| _version_ | 1819279655314653184 |
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| author | M Mobarhan Fard B Vazir M Zendehdel A Asghari |
| author_facet | M Mobarhan Fard B Vazir M Zendehdel A Asghari |
| author_sort | M Mobarhan Fard |
| collection | DOAJ |
| description | This study purposed to discover the connection between the central glutamatergic and histaminergic systems on feeding behavior in layer chickens. In the first experiment, chicks obtained intracerebroventricular (ICV) injections of saline (control solution), α-FMH (250 nmol), glutamate (300 nmol), and α-FMH + glutamate. Experiments 2-6 were comparable to the first experiment, apart from the birds being injected with chlorpheniramine (histamine H1 receptor antagonist, 300 nmol), famotidine (histamine H2 receptor antagonist, 82 nmol), and thioperamide (histamine H3 receptor antagonist, 300 nmol) instead of α-FMH. In Experiment five, experimental groups were divided into (A) control solution, (B) MK-801 (N-methyl-D-aspartate receptor antagonist, 15 nmol), (C) histamine (300 nmol) and (D) MK-801 + histamine. Experiments 6-10 and Experiment five were similar apart from the ICV injections of CNQX (AMPA receptor antagonist, 360 nm), UBP-302 (Kainate receptor antagonist, 390 nm), AIDA (mGluR1 antagonist, 2 nmol), LY341495 (mGluR2 antagonist, 150 nmol), and UBP1112 (mGluR3 antagonist, 2 nmol) given instead of MK-801. Afterward, cumulative food intake was recorded at30, 60, and 120 minutes after the injection process. According to the results, ICV injection of glutamate considerably reduced food intake (p<0.05). Co-injection of α-FMH + glutamate and/or chlorpheniramine + glutamate reduced the hypophagic influence of glutamate (p<0.05), whereas thioperamide + glutamate augmented glutamate-induced hypophagia in neonatal chicks (p<0.05). Co-injection of MK-801 + histamine or UBP-302 + histamine reduced the hypophagic influence of the histamine (p<0.05), whereas LY341495 + histamine augmented the hypophagic influence of the histamine (p<0.05). Given the results, it is suggested that the effect of the connection between these systems on the process of food intake regulation is mediated by H1 and H3 histamines as well as NMDA, Kainate, and mGluR2 glutamate receptors in neonatal layer chickens. |
| first_indexed | 2024-12-24T00:31:21Z |
| format | Article |
| id | doaj.art-33f53cf7aeb2466fb27d77bbd581ed02 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-12-24T00:31:21Z |
| publishDate | 2021-09-01 |
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| series | Archives of Razi Institute |
| spelling | doaj.art-33f53cf7aeb2466fb27d77bbd581ed022022-12-21T17:24:15ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722021-09-0176353755110.22092/ari.2020.351260.1513122236Interaction of Central Glutamatergic and Histaminergic Systems on Food Intake Regulation in Layer ChickensM Mobarhan Fard0B Vazir1M Zendehdel2A Asghari3Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, 14155-6453, Tehran, IranDepartment of Clinical Science, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, IranThis study purposed to discover the connection between the central glutamatergic and histaminergic systems on feeding behavior in layer chickens. In the first experiment, chicks obtained intracerebroventricular (ICV) injections of saline (control solution), α-FMH (250 nmol), glutamate (300 nmol), and α-FMH + glutamate. Experiments 2-6 were comparable to the first experiment, apart from the birds being injected with chlorpheniramine (histamine H1 receptor antagonist, 300 nmol), famotidine (histamine H2 receptor antagonist, 82 nmol), and thioperamide (histamine H3 receptor antagonist, 300 nmol) instead of α-FMH. In Experiment five, experimental groups were divided into (A) control solution, (B) MK-801 (N-methyl-D-aspartate receptor antagonist, 15 nmol), (C) histamine (300 nmol) and (D) MK-801 + histamine. Experiments 6-10 and Experiment five were similar apart from the ICV injections of CNQX (AMPA receptor antagonist, 360 nm), UBP-302 (Kainate receptor antagonist, 390 nm), AIDA (mGluR1 antagonist, 2 nmol), LY341495 (mGluR2 antagonist, 150 nmol), and UBP1112 (mGluR3 antagonist, 2 nmol) given instead of MK-801. Afterward, cumulative food intake was recorded at30, 60, and 120 minutes after the injection process. According to the results, ICV injection of glutamate considerably reduced food intake (p<0.05). Co-injection of α-FMH + glutamate and/or chlorpheniramine + glutamate reduced the hypophagic influence of glutamate (p<0.05), whereas thioperamide + glutamate augmented glutamate-induced hypophagia in neonatal chicks (p<0.05). Co-injection of MK-801 + histamine or UBP-302 + histamine reduced the hypophagic influence of the histamine (p<0.05), whereas LY341495 + histamine augmented the hypophagic influence of the histamine (p<0.05). Given the results, it is suggested that the effect of the connection between these systems on the process of food intake regulation is mediated by H1 and H3 histamines as well as NMDA, Kainate, and mGluR2 glutamate receptors in neonatal layer chickens.https://archrazi.areeo.ac.ir/article_122236_7e323f10ea9e4f2bef48d786f1d71fd5.pdfcentral glutamatehistaminefood intakelayer chicken |
| spellingShingle | M Mobarhan Fard B Vazir M Zendehdel A Asghari Interaction of Central Glutamatergic and Histaminergic Systems on Food Intake Regulation in Layer Chickens Archives of Razi Institute central glutamate histamine food intake layer chicken |
| title | Interaction of Central Glutamatergic and Histaminergic Systems on Food Intake Regulation in Layer Chickens |
| title_full | Interaction of Central Glutamatergic and Histaminergic Systems on Food Intake Regulation in Layer Chickens |
| title_fullStr | Interaction of Central Glutamatergic and Histaminergic Systems on Food Intake Regulation in Layer Chickens |
| title_full_unstemmed | Interaction of Central Glutamatergic and Histaminergic Systems on Food Intake Regulation in Layer Chickens |
| title_short | Interaction of Central Glutamatergic and Histaminergic Systems on Food Intake Regulation in Layer Chickens |
| title_sort | interaction of central glutamatergic and histaminergic systems on food intake regulation in layer chickens |
| topic | central glutamate histamine food intake layer chicken |
| url | https://archrazi.areeo.ac.ir/article_122236_7e323f10ea9e4f2bef48d786f1d71fd5.pdf |
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