Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication
Cultivated melon was domesticated from wild melons, which produce small fruits with non-edible fruit flesh. The increase in fruit flesh is one of the major domestication achievements in this species. In previous work, a quantitative trait locus (QTL) on chromosome 6 (<i>paqt6.1</i>) link...
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MDPI AG
2020-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/8/1064 |
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| author | Chaymaa Riahi Juan Luis Reig-Valiente Belén Picó Aurora Díaz Maria José Gonzalo Antonio José Monforte |
| author_facet | Chaymaa Riahi Juan Luis Reig-Valiente Belén Picó Aurora Díaz Maria José Gonzalo Antonio José Monforte |
| author_sort | Chaymaa Riahi |
| collection | DOAJ |
| description | Cultivated melon was domesticated from wild melons, which produce small fruits with non-edible fruit flesh. The increase in fruit flesh is one of the major domestication achievements in this species. In previous work, a quantitative trait locus (QTL) on chromosome 6 (<i>paqt6.1</i>) linked to fruit flesh content was detected in a cross between cultivated (“Piel de Sapo”, PS) and wild (Ames 24294, TRI) accessions. The QTL was introgressed into the PS background, generating the TRI_6-3 introgression line (IL) that confirmed the effects of <i>paqt6.1</i>. The primary objective of this work was to fine-map <i>paqt6.1</i> as the first step for the map-based cloning. Two different approaches were carried out; however, the results were not consistent, precluding the fine mapping of <i>paqt6.1</i>. TRI_6-3 and other related ILs were genotyped by genotyping-by-sequencing, finding additional introgressions in other chromosomes. In an F<sub>2</sub> population from TRI_6-3-x-PS, we found an epistatic interaction between <i>paqt6.1</i> and another locus on chromosome 11. The interaction was verified in advanced populations, suggesting that the effects of <i>paqt6.1</i> are conditioned by the allelic composition at another locus in chromosome 11. Both loci should have TRI alleles to reduce the flesh content in the PS background. The implications on the history of melon domestication are discussed. |
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| last_indexed | 2024-03-10T18:15:44Z |
| publishDate | 2020-07-01 |
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| series | Agronomy |
| spelling | doaj.art-33f65ee8046547e6a96f4a0fbd5256732023-11-20T07:42:42ZengMDPI AGAgronomy2073-43952020-07-01108106410.3390/agronomy10081064Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during DomesticationChaymaa Riahi0Juan Luis Reig-Valiente1Belén Picó2Aurora Díaz3Maria José Gonzalo4Antonio José Monforte5Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València (UPV)-Consejo Superior de Investigaciones Científicas (CSIC). Ingeniero Fausto Elio s/n, 46022 Valencia, SpainInstituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València (UPV)-Consejo Superior de Investigaciones Científicas (CSIC). Ingeniero Fausto Elio s/n, 46022 Valencia, SpainInstituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de València (COMAV-UPV), Avenida de los Naranjos s/n, 46011 Valencia, SpainInstituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València (UPV)-Consejo Superior de Investigaciones Científicas (CSIC). Ingeniero Fausto Elio s/n, 46022 Valencia, SpainInstituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València (UPV)-Consejo Superior de Investigaciones Científicas (CSIC). Ingeniero Fausto Elio s/n, 46022 Valencia, SpainInstituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València (UPV)-Consejo Superior de Investigaciones Científicas (CSIC). Ingeniero Fausto Elio s/n, 46022 Valencia, SpainCultivated melon was domesticated from wild melons, which produce small fruits with non-edible fruit flesh. The increase in fruit flesh is one of the major domestication achievements in this species. In previous work, a quantitative trait locus (QTL) on chromosome 6 (<i>paqt6.1</i>) linked to fruit flesh content was detected in a cross between cultivated (“Piel de Sapo”, PS) and wild (Ames 24294, TRI) accessions. The QTL was introgressed into the PS background, generating the TRI_6-3 introgression line (IL) that confirmed the effects of <i>paqt6.1</i>. The primary objective of this work was to fine-map <i>paqt6.1</i> as the first step for the map-based cloning. Two different approaches were carried out; however, the results were not consistent, precluding the fine mapping of <i>paqt6.1</i>. TRI_6-3 and other related ILs were genotyped by genotyping-by-sequencing, finding additional introgressions in other chromosomes. In an F<sub>2</sub> population from TRI_6-3-x-PS, we found an epistatic interaction between <i>paqt6.1</i> and another locus on chromosome 11. The interaction was verified in advanced populations, suggesting that the effects of <i>paqt6.1</i> are conditioned by the allelic composition at another locus in chromosome 11. Both loci should have TRI alleles to reduce the flesh content in the PS background. The implications on the history of melon domestication are discussed.https://www.mdpi.com/2073-4395/10/8/1064<i>Cucumis melo</i> L.epistasisQTL cloningfine mappingpericarp |
| spellingShingle | Chaymaa Riahi Juan Luis Reig-Valiente Belén Picó Aurora Díaz Maria José Gonzalo Antonio José Monforte Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication Agronomy <i>Cucumis melo</i> L. epistasis QTL cloning fine mapping pericarp |
| title | Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication |
| title_full | Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication |
| title_fullStr | Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication |
| title_full_unstemmed | Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication |
| title_short | Evidence of the Role of QTL Epistatic Interactions in the Increase of Melon Fruit Flesh Content during Domestication |
| title_sort | evidence of the role of qtl epistatic interactions in the increase of melon fruit flesh content during domestication |
| topic | <i>Cucumis melo</i> L. epistasis QTL cloning fine mapping pericarp |
| url | https://www.mdpi.com/2073-4395/10/8/1064 |
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