Anti-cancer and antimicrobial potential of five soil Streptomycetes: a metabolomics-based study
Lack of new anti-cancer and anti-infective agents directed the pharmaceutical research to natural products' discovery especially from actinomycetes as one of the major sources of bioactive compounds. Metabolomics- and dereplication-guided approach has been used successfully in chemical profilin...
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| Format: | Article |
| Language: | English |
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The Royal Society
2022-02-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsos.211509 |
| _version_ | 1797837625932906496 |
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| author | Nada Osama Walid Bakeer Mai Raslan Hanan A. Soliman Usama Ramadan Abdelmohsen Mohamed Sebak |
| author_facet | Nada Osama Walid Bakeer Mai Raslan Hanan A. Soliman Usama Ramadan Abdelmohsen Mohamed Sebak |
| author_sort | Nada Osama |
| collection | DOAJ |
| description | Lack of new anti-cancer and anti-infective agents directed the pharmaceutical research to natural products' discovery especially from actinomycetes as one of the major sources of bioactive compounds. Metabolomics- and dereplication-guided approach has been used successfully in chemical profiling of bioactive actinomycetes. We aimed to study the metabolomic profile of five bioactive actinomycetes to investigate the interesting metabolites responsible for their antimicrobial and anti-cancer activities. Three actinomycetes, namely, Streptomyces sp. SH8, SH10 and SH13, were found to exhibit broad spectrum of antimicrobial activities, whereas isolate SH4 showed the broadest antimicrobial activity against all tested strains. In addition, isolates SH8, SH10 and SH12 displayed potent cytotoxicity against the breast cancer cell line Michigan Cancer Foundation-7 (MCF-7), whereas isolates SH4 and SH12 exhibited potent anti-cancer activity against the hepatoma cell line hepatoma G2 (HepG2) compared with their weak inhibitory properties on the normal breast cells MCF-10A and normal liver cells transformed human liver epithelial-2 (THLE2), respectively. All bioactive isolates were molecularly identified as Streptomyces sp. via 16S rRNA gene sequencing. Our actinobacterial dereplication analysis revealed putative identification of several bioactive metabolites including tetracycline, oxytetracycline and a macrolide antibiotic, novamethymycin. Together, chemical profiling of bioactive Streptomycetes via dereplication and metabolomics helped in assigning their unique metabolites and predicting the bioactive compounds instigating their diverse bioactivities. |
| first_indexed | 2024-04-09T15:27:42Z |
| format | Article |
| id | doaj.art-33fa0faf1700432594c0c9314590215f |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-04-09T15:27:42Z |
| publishDate | 2022-02-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-33fa0faf1700432594c0c9314590215f2023-04-28T11:04:40ZengThe Royal SocietyRoyal Society Open Science2054-57032022-02-019210.1098/rsos.211509Anti-cancer and antimicrobial potential of five soil Streptomycetes: a metabolomics-based studyNada Osama0Walid Bakeer1Mai Raslan2Hanan A. Soliman3Usama Ramadan Abdelmohsen4Mohamed Sebak5Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, 62511, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62511, EgyptBiotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, 62511, EgyptBiochemistry Division, Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62511, EgyptLack of new anti-cancer and anti-infective agents directed the pharmaceutical research to natural products' discovery especially from actinomycetes as one of the major sources of bioactive compounds. Metabolomics- and dereplication-guided approach has been used successfully in chemical profiling of bioactive actinomycetes. We aimed to study the metabolomic profile of five bioactive actinomycetes to investigate the interesting metabolites responsible for their antimicrobial and anti-cancer activities. Three actinomycetes, namely, Streptomyces sp. SH8, SH10 and SH13, were found to exhibit broad spectrum of antimicrobial activities, whereas isolate SH4 showed the broadest antimicrobial activity against all tested strains. In addition, isolates SH8, SH10 and SH12 displayed potent cytotoxicity against the breast cancer cell line Michigan Cancer Foundation-7 (MCF-7), whereas isolates SH4 and SH12 exhibited potent anti-cancer activity against the hepatoma cell line hepatoma G2 (HepG2) compared with their weak inhibitory properties on the normal breast cells MCF-10A and normal liver cells transformed human liver epithelial-2 (THLE2), respectively. All bioactive isolates were molecularly identified as Streptomyces sp. via 16S rRNA gene sequencing. Our actinobacterial dereplication analysis revealed putative identification of several bioactive metabolites including tetracycline, oxytetracycline and a macrolide antibiotic, novamethymycin. Together, chemical profiling of bioactive Streptomycetes via dereplication and metabolomics helped in assigning their unique metabolites and predicting the bioactive compounds instigating their diverse bioactivities.https://royalsocietypublishing.org/doi/10.1098/rsos.211509actinomycetesanti-cancerantimicrobialdereplicationmetabolomicsnatural products |
| spellingShingle | Nada Osama Walid Bakeer Mai Raslan Hanan A. Soliman Usama Ramadan Abdelmohsen Mohamed Sebak Anti-cancer and antimicrobial potential of five soil Streptomycetes: a metabolomics-based study Royal Society Open Science actinomycetes anti-cancer antimicrobial dereplication metabolomics natural products |
| title | Anti-cancer and antimicrobial potential of five soil Streptomycetes: a metabolomics-based study |
| title_full | Anti-cancer and antimicrobial potential of five soil Streptomycetes: a metabolomics-based study |
| title_fullStr | Anti-cancer and antimicrobial potential of five soil Streptomycetes: a metabolomics-based study |
| title_full_unstemmed | Anti-cancer and antimicrobial potential of five soil Streptomycetes: a metabolomics-based study |
| title_short | Anti-cancer and antimicrobial potential of five soil Streptomycetes: a metabolomics-based study |
| title_sort | anti cancer and antimicrobial potential of five soil streptomycetes a metabolomics based study |
| topic | actinomycetes anti-cancer antimicrobial dereplication metabolomics natural products |
| url | https://royalsocietypublishing.org/doi/10.1098/rsos.211509 |
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