Topical Fibronectin Improves Wound Healing of Irradiated Skin
Abstract Wound healing is significantly delayed in irradiated skin. To better understand global changes in protein expression after radiation, we utilized a reverse phase protein array (RPPA) to identify significant changes in paired samples of normal and irradiated human skin. Of the 210 proteins s...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-03614-y |
| _version_ | 1818840527804563456 |
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| author | Maxwell B. Johnson Brandon Pang Daniel J. Gardner Solmaz Niknam-Benia Vinaya Soundarajan Athanasios Bramos David P. Perrault Kian Banks Gene K. Lee Regina Y. Baker Gene H. Kim Sunju Lee Yang Chai Mei Chen Wei Li Lawrence Kwong Young-Kwon Hong Alex K. Wong |
| author_facet | Maxwell B. Johnson Brandon Pang Daniel J. Gardner Solmaz Niknam-Benia Vinaya Soundarajan Athanasios Bramos David P. Perrault Kian Banks Gene K. Lee Regina Y. Baker Gene H. Kim Sunju Lee Yang Chai Mei Chen Wei Li Lawrence Kwong Young-Kwon Hong Alex K. Wong |
| author_sort | Maxwell B. Johnson |
| collection | DOAJ |
| description | Abstract Wound healing is significantly delayed in irradiated skin. To better understand global changes in protein expression after radiation, we utilized a reverse phase protein array (RPPA) to identify significant changes in paired samples of normal and irradiated human skin. Of the 210 proteins studied, fibronectin was the most significantly and consistently downregulated in radiation-damaged skin. Using a murine model, we confirmed that radiation leads to decreased fibronectin expression in the skin as well as delayed wound healing. Topically applied fibronectin was found to significantly improve wound healing in irradiated skin and was associated with decreased inflammatory infiltrate and increased angiogenesis. Fibronectin treatment may be a useful adjunctive modality in the treatment of non-healing radiation wounds. |
| first_indexed | 2024-12-19T04:11:36Z |
| format | Article |
| id | doaj.art-33fe97d81b974d99b20a3d8bdb3a7a9d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:11:36Z |
| publishDate | 2017-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-33fe97d81b974d99b20a3d8bdb3a7a9d2022-12-21T20:36:24ZengNature PortfolioScientific Reports2045-23222017-06-017111010.1038/s41598-017-03614-yTopical Fibronectin Improves Wound Healing of Irradiated SkinMaxwell B. Johnson0Brandon Pang1Daniel J. Gardner2Solmaz Niknam-Benia3Vinaya Soundarajan4Athanasios Bramos5David P. Perrault6Kian Banks7Gene K. Lee8Regina Y. Baker9Gene H. Kim10Sunju Lee11Yang Chai12Mei Chen13Wei Li14Lawrence Kwong15Young-Kwon Hong16Alex K. Wong17Division of Plastic and Reconstructive Surgery, Keck School of Medicine of USCDivision of Plastic and Reconstructive Surgery, Keck School of Medicine of USCDivision of Plastic and Reconstructive Surgery, Keck School of Medicine of USCDivision of Plastic and Reconstructive Surgery, Keck School of Medicine of USCDivision of Plastic and Reconstructive Surgery, Keck School of Medicine of USCDivision of Plastic and Reconstructive Surgery, Keck School of Medicine of USCDivision of Plastic and Reconstructive Surgery, Keck School of Medicine of USCDivision of Plastic and Reconstructive Surgery, Keck School of Medicine of USCDivision of Plastic and Reconstructive Surgery, Keck School of Medicine of USCDivision of Plastic and Reconstructive Surgery, Keck School of Medicine of USCDepartment of Dermatology, Keck School of Medicine of USCDivision of Plastic and Reconstructive Surgery, Keck School of Medicine of USCCenter for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern CaliforniaDepartment of Dermatology, Keck School of Medicine of USCDepartment of Dermatology, Keck School of Medicine of USCDepartment of Translational Pathology, MD Anderson Cancer CenterDivision of Plastic and Reconstructive Surgery, Keck School of Medicine of USCDivision of Plastic and Reconstructive Surgery, Keck School of Medicine of USCAbstract Wound healing is significantly delayed in irradiated skin. To better understand global changes in protein expression after radiation, we utilized a reverse phase protein array (RPPA) to identify significant changes in paired samples of normal and irradiated human skin. Of the 210 proteins studied, fibronectin was the most significantly and consistently downregulated in radiation-damaged skin. Using a murine model, we confirmed that radiation leads to decreased fibronectin expression in the skin as well as delayed wound healing. Topically applied fibronectin was found to significantly improve wound healing in irradiated skin and was associated with decreased inflammatory infiltrate and increased angiogenesis. Fibronectin treatment may be a useful adjunctive modality in the treatment of non-healing radiation wounds.https://doi.org/10.1038/s41598-017-03614-y |
| spellingShingle | Maxwell B. Johnson Brandon Pang Daniel J. Gardner Solmaz Niknam-Benia Vinaya Soundarajan Athanasios Bramos David P. Perrault Kian Banks Gene K. Lee Regina Y. Baker Gene H. Kim Sunju Lee Yang Chai Mei Chen Wei Li Lawrence Kwong Young-Kwon Hong Alex K. Wong Topical Fibronectin Improves Wound Healing of Irradiated Skin Scientific Reports |
| title | Topical Fibronectin Improves Wound Healing of Irradiated Skin |
| title_full | Topical Fibronectin Improves Wound Healing of Irradiated Skin |
| title_fullStr | Topical Fibronectin Improves Wound Healing of Irradiated Skin |
| title_full_unstemmed | Topical Fibronectin Improves Wound Healing of Irradiated Skin |
| title_short | Topical Fibronectin Improves Wound Healing of Irradiated Skin |
| title_sort | topical fibronectin improves wound healing of irradiated skin |
| url | https://doi.org/10.1038/s41598-017-03614-y |
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