A Molecular Signature of Two Long Non-Coding RNAs in Peripheral Blood Predicts Acute Renal Allograft Rejection
Background/Aims: Acute rejection (AR) is a major complication post renal transplantation, with no widely-accepted non-invasive biomarker. This study aimed to explore the expression profiles of long non-coding RNAs (lncRNAs) in the peripheral blood (PB) of renal transplant recipients and their potent...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2017-11-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | https://www.karger.com/Article/FullText/485451 |
| _version_ | 1819132933003280384 |
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| author | Yu-Zheng Ge Tao Xu Wei-Jun Cao Ran Wu Wen-Tao Yao Chang-Cheng Zhou Min Wang Lu-Wei Xu Tian-Ze Lu You-Cai Zhao Zhi-Kai Hu Zhong-Le Xu Xiao-Bing Yang Liu-Hua Zhou Sheng-Li Zhang Bing Zhong Zheng Xu Wen-Cheng Li Jia-Geng Zhu Rui-Peng Jia |
| author_facet | Yu-Zheng Ge Tao Xu Wei-Jun Cao Ran Wu Wen-Tao Yao Chang-Cheng Zhou Min Wang Lu-Wei Xu Tian-Ze Lu You-Cai Zhao Zhi-Kai Hu Zhong-Le Xu Xiao-Bing Yang Liu-Hua Zhou Sheng-Li Zhang Bing Zhong Zheng Xu Wen-Cheng Li Jia-Geng Zhu Rui-Peng Jia |
| author_sort | Yu-Zheng Ge |
| collection | DOAJ |
| description | Background/Aims: Acute rejection (AR) is a major complication post renal transplantation, with no widely-accepted non-invasive biomarker. This study aimed to explore the expression profiles of long non-coding RNAs (lncRNAs) in the peripheral blood (PB) of renal transplant recipients and their potential diagnostic values. Methods: The genome-wide lncRNA expression profiles were analyzed in 150 PB samples from pediatric and adult renal transplant (PRTx and ARTx) cohorts. The diagnostic performance of differentially expressed lncRNA was determined using receiver operator characteristic curve, with area under the curve (AUC) and 95% confidential interval (CI). Finally, a risk score was constructed with logistical regression model. Results: A total of 162 lncRNAs were found differentially expressed in PRTx cohort, while 163 in ARTx cohort. Among these identified lncRNAs, 23 deregulated accordingly in both cohorts, and could distinguish AR recipients from those without AR. Finally, a risk score with two most significant lncRNAs (AF264622 and AB209021) was generated and exhibited excellent diagnostic performance in both PRTx (AUC:0.829, 95% CI:0.735-0.922) and ARTx cohorts (AUC: 0.889, 95% CI: 0.817-0.960). Conclusion: A molecular signature of two lncRNAs in PB could serve as a novel non-invasive biomarker for the diagnosis of AR in both pediatric and adult renal transplant recipients. |
| first_indexed | 2024-12-22T09:39:16Z |
| format | Article |
| id | doaj.art-33ffc787940341cd91a7bbf20c02d918 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-22T09:39:16Z |
| publishDate | 2017-11-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-33ffc787940341cd91a7bbf20c02d9182022-12-21T18:30:44ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-11-014431213122310.1159/000485451485451A Molecular Signature of Two Long Non-Coding RNAs in Peripheral Blood Predicts Acute Renal Allograft RejectionYu-Zheng GeTao XuWei-Jun CaoRan WuWen-Tao YaoChang-Cheng ZhouMin WangLu-Wei XuTian-Ze LuYou-Cai ZhaoZhi-Kai HuZhong-Le XuXiao-Bing YangLiu-Hua ZhouSheng-Li ZhangBing ZhongZheng XuWen-Cheng LiJia-Geng ZhuRui-Peng JiaBackground/Aims: Acute rejection (AR) is a major complication post renal transplantation, with no widely-accepted non-invasive biomarker. This study aimed to explore the expression profiles of long non-coding RNAs (lncRNAs) in the peripheral blood (PB) of renal transplant recipients and their potential diagnostic values. Methods: The genome-wide lncRNA expression profiles were analyzed in 150 PB samples from pediatric and adult renal transplant (PRTx and ARTx) cohorts. The diagnostic performance of differentially expressed lncRNA was determined using receiver operator characteristic curve, with area under the curve (AUC) and 95% confidential interval (CI). Finally, a risk score was constructed with logistical regression model. Results: A total of 162 lncRNAs were found differentially expressed in PRTx cohort, while 163 in ARTx cohort. Among these identified lncRNAs, 23 deregulated accordingly in both cohorts, and could distinguish AR recipients from those without AR. Finally, a risk score with two most significant lncRNAs (AF264622 and AB209021) was generated and exhibited excellent diagnostic performance in both PRTx (AUC:0.829, 95% CI:0.735-0.922) and ARTx cohorts (AUC: 0.889, 95% CI: 0.817-0.960). Conclusion: A molecular signature of two lncRNAs in PB could serve as a novel non-invasive biomarker for the diagnosis of AR in both pediatric and adult renal transplant recipients.https://www.karger.com/Article/FullText/485451Renal transplantAcute rejectionLong non-coding RNADiagnosisBiomarker |
| spellingShingle | Yu-Zheng Ge Tao Xu Wei-Jun Cao Ran Wu Wen-Tao Yao Chang-Cheng Zhou Min Wang Lu-Wei Xu Tian-Ze Lu You-Cai Zhao Zhi-Kai Hu Zhong-Le Xu Xiao-Bing Yang Liu-Hua Zhou Sheng-Li Zhang Bing Zhong Zheng Xu Wen-Cheng Li Jia-Geng Zhu Rui-Peng Jia A Molecular Signature of Two Long Non-Coding RNAs in Peripheral Blood Predicts Acute Renal Allograft Rejection Cellular Physiology and Biochemistry Renal transplant Acute rejection Long non-coding RNA Diagnosis Biomarker |
| title | A Molecular Signature of Two Long Non-Coding RNAs in Peripheral Blood Predicts Acute Renal Allograft Rejection |
| title_full | A Molecular Signature of Two Long Non-Coding RNAs in Peripheral Blood Predicts Acute Renal Allograft Rejection |
| title_fullStr | A Molecular Signature of Two Long Non-Coding RNAs in Peripheral Blood Predicts Acute Renal Allograft Rejection |
| title_full_unstemmed | A Molecular Signature of Two Long Non-Coding RNAs in Peripheral Blood Predicts Acute Renal Allograft Rejection |
| title_short | A Molecular Signature of Two Long Non-Coding RNAs in Peripheral Blood Predicts Acute Renal Allograft Rejection |
| title_sort | molecular signature of two long non coding rnas in peripheral blood predicts acute renal allograft rejection |
| topic | Renal transplant Acute rejection Long non-coding RNA Diagnosis Biomarker |
| url | https://www.karger.com/Article/FullText/485451 |
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