Mannitol improves Monascus pigment biosynthesis with rice bran as a substrate in Monascus purpureus
To reduce the production cost of Monascus pigments (MPs), the utilization of rice bran (RB), an agricultural waste product, as a substrate in submerged fermentation was conducted in this study. To improve MP production, different nutritional ingredients including mannitol (Man), NH4NO3 (AN), ZnSO4 (...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1300461/full |
| _version_ | 1827585854119346176 |
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| author | Di Chen Han Li |
| author_facet | Di Chen Han Li |
| author_sort | Di Chen |
| collection | DOAJ |
| description | To reduce the production cost of Monascus pigments (MPs), the utilization of rice bran (RB), an agricultural waste product, as a substrate in submerged fermentation was conducted in this study. To improve MP production, different nutritional ingredients including mannitol (Man), NH4NO3 (AN), ZnSO4 (Zn), and optimization (Opti), which was a synthesis of the three above ones, were added in rice bran (RB) medium. The yields of MPs, pigment constituents, and growth and development of Monascus purpureus M9 were investigated in this study. Man had the maximum color value of 3,532 U/g, which was 18.69 times more than that of RB and reached up to 76.65% of the value of rice (Rice) fermentation. Man significantly increased the production of two orange pigments, monascorubrin and rubropunctatin, of which the yields were 69.49 and 95.36% of the counterpart of Rice. The biomass and colony diameter of Opti presented the maximum value among different groups. AN and RB induced more asexual spore formation, whereas Opti and Man promoted sexual spore production. Comparative transcriptomic analysis showed that different nutritional ingredients led to changes in pigment production, promoting the growth and development of M. purpureus M9 through the regulation of related gene expression. Man and Opti improved MP production by regulating the primary metabolism, including the Embden–Meyerhof pathway (EMP), the pentose phosphate (PP) pathway, the tricarboxylic (TCA) cycle, fatty acid degradation (FAD), fatty acid biosynthesis (FAB), amino acid metabolism (AAM), and fructose and mannose metabolism (FMM), to provide the precursors (acetyl-CoA and malonyl-CoA) for MP biosynthesis. This study presents a low-cost method for increasing MP production and explains the molecular mechanisms of different nutritional ingredients for enhancing MP biosynthesis. |
| first_indexed | 2024-03-08T23:52:34Z |
| format | Article |
| id | doaj.art-3401cf0385fa41429cc88284551196dc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T23:52:34Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-3401cf0385fa41429cc88284551196dc2023-12-13T10:01:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-12-011410.3389/fmicb.2023.13004611300461Mannitol improves Monascus pigment biosynthesis with rice bran as a substrate in Monascus purpureusDi ChenHan LiTo reduce the production cost of Monascus pigments (MPs), the utilization of rice bran (RB), an agricultural waste product, as a substrate in submerged fermentation was conducted in this study. To improve MP production, different nutritional ingredients including mannitol (Man), NH4NO3 (AN), ZnSO4 (Zn), and optimization (Opti), which was a synthesis of the three above ones, were added in rice bran (RB) medium. The yields of MPs, pigment constituents, and growth and development of Monascus purpureus M9 were investigated in this study. Man had the maximum color value of 3,532 U/g, which was 18.69 times more than that of RB and reached up to 76.65% of the value of rice (Rice) fermentation. Man significantly increased the production of two orange pigments, monascorubrin and rubropunctatin, of which the yields were 69.49 and 95.36% of the counterpart of Rice. The biomass and colony diameter of Opti presented the maximum value among different groups. AN and RB induced more asexual spore formation, whereas Opti and Man promoted sexual spore production. Comparative transcriptomic analysis showed that different nutritional ingredients led to changes in pigment production, promoting the growth and development of M. purpureus M9 through the regulation of related gene expression. Man and Opti improved MP production by regulating the primary metabolism, including the Embden–Meyerhof pathway (EMP), the pentose phosphate (PP) pathway, the tricarboxylic (TCA) cycle, fatty acid degradation (FAD), fatty acid biosynthesis (FAB), amino acid metabolism (AAM), and fructose and mannose metabolism (FMM), to provide the precursors (acetyl-CoA and malonyl-CoA) for MP biosynthesis. This study presents a low-cost method for increasing MP production and explains the molecular mechanisms of different nutritional ingredients for enhancing MP biosynthesis.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1300461/fullMonascus pigmentsrice brannutritional ingredientsgrowth and developmenttranscriptome analysis |
| spellingShingle | Di Chen Han Li Mannitol improves Monascus pigment biosynthesis with rice bran as a substrate in Monascus purpureus Frontiers in Microbiology Monascus pigments rice bran nutritional ingredients growth and development transcriptome analysis |
| title | Mannitol improves Monascus pigment biosynthesis with rice bran as a substrate in Monascus purpureus |
| title_full | Mannitol improves Monascus pigment biosynthesis with rice bran as a substrate in Monascus purpureus |
| title_fullStr | Mannitol improves Monascus pigment biosynthesis with rice bran as a substrate in Monascus purpureus |
| title_full_unstemmed | Mannitol improves Monascus pigment biosynthesis with rice bran as a substrate in Monascus purpureus |
| title_short | Mannitol improves Monascus pigment biosynthesis with rice bran as a substrate in Monascus purpureus |
| title_sort | mannitol improves monascus pigment biosynthesis with rice bran as a substrate in monascus purpureus |
| topic | Monascus pigments rice bran nutritional ingredients growth and development transcriptome analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1300461/full |
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