Composition of Triterpenoids in <i>Inonotus obliquus</i> and Their Anti-Proliferative Activity on Cancer Cell Lines
The objective of this study was to determine the composition of triterpenoids in the extracts from the inner and outer parts of <i>Inonotus obliquus</i> and to evaluate their anti-proliferative activity against cancer cell lines (HT-29, AGS, MCF-7, and PC3). Inner and outer parts of <...
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MDPI AG
2020-09-01
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author | Jaecheol Kim Si Chang Yang Ah Young Hwang Hyunnho Cho Keum Taek Hwang |
author_facet | Jaecheol Kim Si Chang Yang Ah Young Hwang Hyunnho Cho Keum Taek Hwang |
author_sort | Jaecheol Kim |
collection | DOAJ |
description | The objective of this study was to determine the composition of triterpenoids in the extracts from the inner and outer parts of <i>Inonotus obliquus</i> and to evaluate their anti-proliferative activity against cancer cell lines (HT-29, AGS, MCF-7, and PC3). Inner and outer parts of <i>I. obliquus</i> were extracted with 80% methanol for 24 h. The extract was fractionated by Diaion HP-20 resin to obtain the triterpenoid fraction. Composition of triterpenoids in the fraction was analyzed by HPLC and LC-ESI-MS. Anti-proliferative activity was evaluated by MTT assay against cancer cell lines. Inotodiol and trametenolic acid were major triterpenoids in both of the inner and outer parts of <i>I. obliquus</i>. Inotodiol in triterpenoid fractions from the inner and outer parts of <i>I. obliquus</i> was 153.9 ± 15.4 mg/g (dry basis (db)) and 194.1 ± 11.5 mg/g, respectively. Trametenolic acid in triterpenoid fractions from the inner and outer parts of <i>I. obliquus</i> was 94.5 ± 9.15 mg/g (db) and 106.3 ± 8.23 mg/g, respectively. Triterpenoids in the outer part were significantly higher than those in the inner part. Anti-proliferative activity of the triterpenoid fraction from the outer part against AGS, MCF-7, and PC3 was also significantly higher than that of the inner part. |
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spelling | doaj.art-340454a67da149eabec126acaf49f87c2023-11-20T12:44:13ZengMDPI AGMolecules1420-30492020-09-012518406610.3390/molecules25184066Composition of Triterpenoids in <i>Inonotus obliquus</i> and Their Anti-Proliferative Activity on Cancer Cell LinesJaecheol Kim0Si Chang Yang1Ah Young Hwang2Hyunnho Cho3Keum Taek Hwang4Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, KoreaDepartment of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, KoreaDepartment of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, KoreaFood Analysis Team, Busan Metropolitan City Institute of Health and Environment, Busan 46616, KoreaDepartment of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, KoreaThe objective of this study was to determine the composition of triterpenoids in the extracts from the inner and outer parts of <i>Inonotus obliquus</i> and to evaluate their anti-proliferative activity against cancer cell lines (HT-29, AGS, MCF-7, and PC3). Inner and outer parts of <i>I. obliquus</i> were extracted with 80% methanol for 24 h. The extract was fractionated by Diaion HP-20 resin to obtain the triterpenoid fraction. Composition of triterpenoids in the fraction was analyzed by HPLC and LC-ESI-MS. Anti-proliferative activity was evaluated by MTT assay against cancer cell lines. Inotodiol and trametenolic acid were major triterpenoids in both of the inner and outer parts of <i>I. obliquus</i>. Inotodiol in triterpenoid fractions from the inner and outer parts of <i>I. obliquus</i> was 153.9 ± 15.4 mg/g (dry basis (db)) and 194.1 ± 11.5 mg/g, respectively. Trametenolic acid in triterpenoid fractions from the inner and outer parts of <i>I. obliquus</i> was 94.5 ± 9.15 mg/g (db) and 106.3 ± 8.23 mg/g, respectively. Triterpenoids in the outer part were significantly higher than those in the inner part. Anti-proliferative activity of the triterpenoid fraction from the outer part against AGS, MCF-7, and PC3 was also significantly higher than that of the inner part.https://www.mdpi.com/1420-3049/25/18/4066<i>Inonotus obliquus</i>chaga mushroomtriterpenoidscancer cellsanti-proliferative activity |
spellingShingle | Jaecheol Kim Si Chang Yang Ah Young Hwang Hyunnho Cho Keum Taek Hwang Composition of Triterpenoids in <i>Inonotus obliquus</i> and Their Anti-Proliferative Activity on Cancer Cell Lines Molecules <i>Inonotus obliquus</i> chaga mushroom triterpenoids cancer cells anti-proliferative activity |
title | Composition of Triterpenoids in <i>Inonotus obliquus</i> and Their Anti-Proliferative Activity on Cancer Cell Lines |
title_full | Composition of Triterpenoids in <i>Inonotus obliquus</i> and Their Anti-Proliferative Activity on Cancer Cell Lines |
title_fullStr | Composition of Triterpenoids in <i>Inonotus obliquus</i> and Their Anti-Proliferative Activity on Cancer Cell Lines |
title_full_unstemmed | Composition of Triterpenoids in <i>Inonotus obliquus</i> and Their Anti-Proliferative Activity on Cancer Cell Lines |
title_short | Composition of Triterpenoids in <i>Inonotus obliquus</i> and Their Anti-Proliferative Activity on Cancer Cell Lines |
title_sort | composition of triterpenoids in i inonotus obliquus i and their anti proliferative activity on cancer cell lines |
topic | <i>Inonotus obliquus</i> chaga mushroom triterpenoids cancer cells anti-proliferative activity |
url | https://www.mdpi.com/1420-3049/25/18/4066 |
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