Protease activity in the excretory-secretory products of nymphal stage of Linguatula serrata: First detection of a metalloprotease.
The aim of this study was to evaluate the presence of proteases and determine the main protease pre-sent in the excretory-secretory products (ESPs) from nymphal stage of Linguatula serrata. Infected mesenteric lymph nodes of goats were collected from Tabriz slaughterhouse, northwestern Iran. Re-cove...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
2023-03-01
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| Series: | Bulgarian Journal of Veterinary Medicine |
| Subjects: |
| Summary: | The aim of this study was to evaluate the presence of proteases and determine the main protease pre-sent in the excretory-secretory products (ESPs) from nymphal stage of Linguatula serrata. Infected mesenteric lymph nodes of goats were collected from Tabriz slaughterhouse, northwestern Iran. Re-covered Linguatula serrata nymphs were immersed in culture medium (MEM), then ESPs were col-lected and protease activity in presence of specific inhibitors was assayed. Protease enzyme was fur-ther characterised by SDS-PAGE. The results of this study showed that the main protease in the ESPs from the nymphal stage of L. serrata was a metalloprotease that was resistant to heat. In conclusion, these data show that a major protease secreted by the larval stage of L. serrata exhibited properties that may play a role in the pathogenesis of L. serrata nymphs. |
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| ISSN: | 1311-1477 1313-3543 |