Protease activity in the excretory-secretory products of nymphal stage of Linguatula serrata: First detection of a metalloprotease.
The aim of this study was to evaluate the presence of proteases and determine the main protease pre-sent in the excretory-secretory products (ESPs) from nymphal stage of Linguatula serrata. Infected mesenteric lymph nodes of goats were collected from Tabriz slaughterhouse, northwestern Iran. Re-cove...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
2023-03-01
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| Series: | Bulgarian Journal of Veterinary Medicine |
| Subjects: |
| _version_ | 1797948911411789824 |
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| author | N. Hajipour M. Tavassoli H. Tayefi-Nasrabadi |
| author_facet | N. Hajipour M. Tavassoli H. Tayefi-Nasrabadi |
| author_sort | N. Hajipour |
| collection | DOAJ |
| description | The aim of this study was to evaluate the presence of proteases and determine the main protease pre-sent in the excretory-secretory products (ESPs) from nymphal stage of Linguatula serrata. Infected mesenteric lymph nodes of goats were collected from Tabriz slaughterhouse, northwestern Iran. Re-covered Linguatula serrata nymphs were immersed in culture medium (MEM), then ESPs were col-lected and protease activity in presence of specific inhibitors was assayed. Protease enzyme was fur-ther characterised by SDS-PAGE. The results of this study showed that the main protease in the ESPs from the nymphal stage of L. serrata was a metalloprotease that was resistant to heat. In conclusion, these data show that a major protease secreted by the larval stage of L. serrata exhibited properties that may play a role in the pathogenesis of L. serrata nymphs. |
| first_indexed | 2024-04-10T21:51:59Z |
| format | Article |
| id | doaj.art-34091a9f03dd4b688e604f6d5434cac5 |
| institution | Directory Open Access Journal |
| issn | 1311-1477 1313-3543 |
| language | English |
| last_indexed | 2024-04-10T21:51:59Z |
| publishDate | 2023-03-01 |
| publisher | Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria |
| record_format | Article |
| series | Bulgarian Journal of Veterinary Medicine |
| spelling | doaj.art-34091a9f03dd4b688e604f6d5434cac52023-01-18T12:38:10ZengFaculty of Veterinary Medicine, Trakia University, Stara Zagora, BulgariaBulgarian Journal of Veterinary Medicine1311-14771313-35432023-03-01261899610.15547/bjvm.2414Protease activity in the excretory-secretory products of nymphal stage of Linguatula serrata: First detection of a metalloprotease.N. Hajipour0M. Tavassoli1H. Tayefi-Nasrabadi2Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranFaculty of Veterinary Medicine, Urmia University, Urmia, IranFaculty of Veterinary Medicine, University of Tabriz, Tabriz, IranThe aim of this study was to evaluate the presence of proteases and determine the main protease pre-sent in the excretory-secretory products (ESPs) from nymphal stage of Linguatula serrata. Infected mesenteric lymph nodes of goats were collected from Tabriz slaughterhouse, northwestern Iran. Re-covered Linguatula serrata nymphs were immersed in culture medium (MEM), then ESPs were col-lected and protease activity in presence of specific inhibitors was assayed. Protease enzyme was fur-ther characterised by SDS-PAGE. The results of this study showed that the main protease in the ESPs from the nymphal stage of L. serrata was a metalloprotease that was resistant to heat. In conclusion, these data show that a major protease secreted by the larval stage of L. serrata exhibited properties that may play a role in the pathogenesis of L. serrata nymphs. linguatula serrataproteasemetalloproteasezoonotic |
| spellingShingle | N. Hajipour M. Tavassoli H. Tayefi-Nasrabadi Protease activity in the excretory-secretory products of nymphal stage of Linguatula serrata: First detection of a metalloprotease. Bulgarian Journal of Veterinary Medicine linguatula serrata protease metalloprotease zoonotic |
| title | Protease activity in the excretory-secretory products of nymphal stage of Linguatula serrata: First detection of a metalloprotease. |
| title_full | Protease activity in the excretory-secretory products of nymphal stage of Linguatula serrata: First detection of a metalloprotease. |
| title_fullStr | Protease activity in the excretory-secretory products of nymphal stage of Linguatula serrata: First detection of a metalloprotease. |
| title_full_unstemmed | Protease activity in the excretory-secretory products of nymphal stage of Linguatula serrata: First detection of a metalloprotease. |
| title_short | Protease activity in the excretory-secretory products of nymphal stage of Linguatula serrata: First detection of a metalloprotease. |
| title_sort | protease activity in the excretory secretory products of nymphal stage of linguatula serrata first detection of a metalloprotease |
| topic | linguatula serrata protease metalloprotease zoonotic |
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