Exogenous IAA application affects the specific characteristics of fluoranthene distribution in Arabidopsis
Indole-3-acetic acid (IAA) is a crucial growth regulator involved in the accumulation of polycyclic aromatic hydrocarbons (PAHs). However, the precise physiological and molecular mechanisms underlying IAA-mediated plant growth and PAH accumulation are not yet fully understood. In this study, two dis...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323008102 |
| _version_ | 1797683189766946816 |
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| author | Mengjia Zhou Ji Wang Jing Zhou Lin Liu Ruixuan Yang Jingjing Xu Mingxiang Liang Li Xu |
| author_facet | Mengjia Zhou Ji Wang Jing Zhou Lin Liu Ruixuan Yang Jingjing Xu Mingxiang Liang Li Xu |
| author_sort | Mengjia Zhou |
| collection | DOAJ |
| description | Indole-3-acetic acid (IAA) is a crucial growth regulator involved in the accumulation of polycyclic aromatic hydrocarbons (PAHs). However, the precise physiological and molecular mechanisms underlying IAA-mediated plant growth and PAH accumulation are not yet fully understood. In this study, two distinct IAA-sensitive genotypes of Arabidopsis thaliana (wild type and Axr5 mutant) were chosen to investigate the mechanisms of fluoranthene (Flu) uptake and accumulation in plant tissues (roots and leaves) through physiological and molecular analyses. The results revealed that the Flu concentration in Axr5 leaves was significantly higher than that in wild-type (WT) leaves. In roots, the Flu content decreased significantly with increasing IAA treatment, while no significant changes were observed with lower IAA treatment. Principal component analysis demonstrated that Flu accumulation in Arabidopsis roots was associated with IAA concentrations, whereas Flu accumulation in leaves was dependent on the genotype. Moreover, Flu accumulation showed a positive correlation with the activity of glutathione S-transferase (GST) and root length and a positive correlation with catalase (CAT) and peroxidase (POD) activity in the leaves. Transcriptome analysis confirmed that the expression of the ethylene-related gene ATERF6 and GST-related genes ATGSTF14 and ATGSTU27 in roots, as well as the POD-related genes AtPRX9 and AtPRX25 and CAT-related gene AtCAT3 in leaves, played a role in Flu accumulation. Furthermore, WRKY transcription factors (TFs) in roots and NAC TFs in leaves were identified as important regulators of Flu accumulation. Understanding the mechanisms of Flu uptake and accumulation in A. thaliana provides valuable insights for regulating PAH accumulation in plants. |
| first_indexed | 2024-03-12T00:10:56Z |
| format | Article |
| id | doaj.art-3409f7dfa90f4b13b30463b22739ea2d |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-12T00:10:56Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-3409f7dfa90f4b13b30463b22739ea2d2023-09-16T05:28:29ZengElsevierEcotoxicology and Environmental Safety0147-65132023-09-01263115306Exogenous IAA application affects the specific characteristics of fluoranthene distribution in ArabidopsisMengjia Zhou0Ji Wang1Jing Zhou2Lin Liu3Ruixuan Yang4Jingjing Xu5Mingxiang Liang6Li Xu7Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210014, ChinaSoil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaSoil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210014, ChinaSoil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaSoil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaSoil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaSoil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaSoil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210014, China; Correspondence to: Weigang No. 1, Nanjing, Jiangsu Province 210095, People's Republic of China.Indole-3-acetic acid (IAA) is a crucial growth regulator involved in the accumulation of polycyclic aromatic hydrocarbons (PAHs). However, the precise physiological and molecular mechanisms underlying IAA-mediated plant growth and PAH accumulation are not yet fully understood. In this study, two distinct IAA-sensitive genotypes of Arabidopsis thaliana (wild type and Axr5 mutant) were chosen to investigate the mechanisms of fluoranthene (Flu) uptake and accumulation in plant tissues (roots and leaves) through physiological and molecular analyses. The results revealed that the Flu concentration in Axr5 leaves was significantly higher than that in wild-type (WT) leaves. In roots, the Flu content decreased significantly with increasing IAA treatment, while no significant changes were observed with lower IAA treatment. Principal component analysis demonstrated that Flu accumulation in Arabidopsis roots was associated with IAA concentrations, whereas Flu accumulation in leaves was dependent on the genotype. Moreover, Flu accumulation showed a positive correlation with the activity of glutathione S-transferase (GST) and root length and a positive correlation with catalase (CAT) and peroxidase (POD) activity in the leaves. Transcriptome analysis confirmed that the expression of the ethylene-related gene ATERF6 and GST-related genes ATGSTF14 and ATGSTU27 in roots, as well as the POD-related genes AtPRX9 and AtPRX25 and CAT-related gene AtCAT3 in leaves, played a role in Flu accumulation. Furthermore, WRKY transcription factors (TFs) in roots and NAC TFs in leaves were identified as important regulators of Flu accumulation. Understanding the mechanisms of Flu uptake and accumulation in A. thaliana provides valuable insights for regulating PAH accumulation in plants.http://www.sciencedirect.com/science/article/pii/S0147651323008102FluorantheneIndole-3-acetic acidTranscriptomeArabidopsis thaliana |
| spellingShingle | Mengjia Zhou Ji Wang Jing Zhou Lin Liu Ruixuan Yang Jingjing Xu Mingxiang Liang Li Xu Exogenous IAA application affects the specific characteristics of fluoranthene distribution in Arabidopsis Ecotoxicology and Environmental Safety Fluoranthene Indole-3-acetic acid Transcriptome Arabidopsis thaliana |
| title | Exogenous IAA application affects the specific characteristics of fluoranthene distribution in Arabidopsis |
| title_full | Exogenous IAA application affects the specific characteristics of fluoranthene distribution in Arabidopsis |
| title_fullStr | Exogenous IAA application affects the specific characteristics of fluoranthene distribution in Arabidopsis |
| title_full_unstemmed | Exogenous IAA application affects the specific characteristics of fluoranthene distribution in Arabidopsis |
| title_short | Exogenous IAA application affects the specific characteristics of fluoranthene distribution in Arabidopsis |
| title_sort | exogenous iaa application affects the specific characteristics of fluoranthene distribution in arabidopsis |
| topic | Fluoranthene Indole-3-acetic acid Transcriptome Arabidopsis thaliana |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323008102 |
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