Characterization of the phosphofructokinase gene family in rice and its expression under oxygen deficiency stress
Plants possess two types of phosphofructokinase proteins for phosphorylation of fructose-6-phosphate, the ATP-dependent phosphofructokinase (PFK) and the pyrophosphate-(PPi) dependent pyrophosphate-fructose-6-phosphate-phosphotransferase (PFP). During oxygen deficiency ATP levels in rice seedlings a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00125/full |
| _version_ | 1818518033076846592 |
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| author | Angelika eMustroph Johanna eStock Natalia eHess Sophia eAldous Sophia eAldous Anika eDreilich Bernhard eGrimm |
| author_facet | Angelika eMustroph Johanna eStock Natalia eHess Sophia eAldous Sophia eAldous Anika eDreilich Bernhard eGrimm |
| author_sort | Angelika eMustroph |
| collection | DOAJ |
| description | Plants possess two types of phosphofructokinase proteins for phosphorylation of fructose-6-phosphate, the ATP-dependent phosphofructokinase (PFK) and the pyrophosphate-(PPi) dependent pyrophosphate-fructose-6-phosphate-phosphotransferase (PFP). During oxygen deficiency ATP levels in rice seedlings are severely reduced, and it is hypothesized that PPi is used as an alternative energy source for the phosphorylation of fructose-6-phosphate during glycolysis. In this study, we analyzed the expression of 15 phosphofructokinase-encoding genes in roots and aerial tissues of anoxia-tolerant rice seedlings in response to anoxic stress and compared our data with transcript profiles obtained from microarray analyses. Furthermore, the intracellular localization of rice PFK proteins was determined, and the PFK and PFP isoforms were grouped in a phylogenetic tree. Two PFK and two PFP transcripts accumulated during anoxic stress, whereas mRNA levels of four PFK and three PFP genes were decreased. The total specific activity of both PFK and PFP changed only slightly during a 24 h anoxia treatment. It is assumed that expression of different isoforms and their catalytic properties differ during normoxic and anoxic conditions and contribute to balanced glycolytic activity during the low oxygen stress. These characterizations of phosphofructokinase genes and the comparison to other plant species allowed us to suggest candidate rice genes for adaptation to anoxic stress. |
| first_indexed | 2024-12-11T01:04:30Z |
| format | Article |
| id | doaj.art-34139c2b39684537a8ecde6b87337474 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T01:04:30Z |
| publishDate | 2013-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-34139c2b39684537a8ecde6b873374742022-12-22T01:26:13ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2013-05-01410.3389/fpls.2013.0012547628Characterization of the phosphofructokinase gene family in rice and its expression under oxygen deficiency stressAngelika eMustroph0Johanna eStock1Natalia eHess2Sophia eAldous3Sophia eAldous4Anika eDreilich5Bernhard eGrimm6University BayreuthUniversity BayreuthUniversity BayreuthHumboldt-University BerlinHeinrich-Heine-University DüsseldorfHumboldt-University BerlinHumboldt-University BerlinPlants possess two types of phosphofructokinase proteins for phosphorylation of fructose-6-phosphate, the ATP-dependent phosphofructokinase (PFK) and the pyrophosphate-(PPi) dependent pyrophosphate-fructose-6-phosphate-phosphotransferase (PFP). During oxygen deficiency ATP levels in rice seedlings are severely reduced, and it is hypothesized that PPi is used as an alternative energy source for the phosphorylation of fructose-6-phosphate during glycolysis. In this study, we analyzed the expression of 15 phosphofructokinase-encoding genes in roots and aerial tissues of anoxia-tolerant rice seedlings in response to anoxic stress and compared our data with transcript profiles obtained from microarray analyses. Furthermore, the intracellular localization of rice PFK proteins was determined, and the PFK and PFP isoforms were grouped in a phylogenetic tree. Two PFK and two PFP transcripts accumulated during anoxic stress, whereas mRNA levels of four PFK and three PFP genes were decreased. The total specific activity of both PFK and PFP changed only slightly during a 24 h anoxia treatment. It is assumed that expression of different isoforms and their catalytic properties differ during normoxic and anoxic conditions and contribute to balanced glycolytic activity during the low oxygen stress. These characterizations of phosphofructokinase genes and the comparison to other plant species allowed us to suggest candidate rice genes for adaptation to anoxic stress.http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00125/fullAnoxiaOryza sativasubmergencephosphofructokinasepyrophosphate |
| spellingShingle | Angelika eMustroph Johanna eStock Natalia eHess Sophia eAldous Sophia eAldous Anika eDreilich Bernhard eGrimm Characterization of the phosphofructokinase gene family in rice and its expression under oxygen deficiency stress Frontiers in Plant Science Anoxia Oryza sativa submergence phosphofructokinase pyrophosphate |
| title | Characterization of the phosphofructokinase gene family in rice and its expression under oxygen deficiency stress |
| title_full | Characterization of the phosphofructokinase gene family in rice and its expression under oxygen deficiency stress |
| title_fullStr | Characterization of the phosphofructokinase gene family in rice and its expression under oxygen deficiency stress |
| title_full_unstemmed | Characterization of the phosphofructokinase gene family in rice and its expression under oxygen deficiency stress |
| title_short | Characterization of the phosphofructokinase gene family in rice and its expression under oxygen deficiency stress |
| title_sort | characterization of the phosphofructokinase gene family in rice and its expression under oxygen deficiency stress |
| topic | Anoxia Oryza sativa submergence phosphofructokinase pyrophosphate |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00125/full |
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