Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region
The plant cuticle has traditionally been conceived as an independent hydrophobic layer that covers the external epidermal cell wall. Due to its complexity, the existing relationship between cuticle chemical composition and ultra-structure remains unclear to date. This study aimed to examine the link...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-09-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00481/full |
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| author | Paula eGuzmán Victoria eFernandez José eGraça Vanessa eCabral Nour eKayali Mohamed eKhayet Luis eGil |
| author_facet | Paula eGuzmán Victoria eFernandez José eGraça Vanessa eCabral Nour eKayali Mohamed eKhayet Luis eGil |
| author_sort | Paula eGuzmán |
| collection | DOAJ |
| description | The plant cuticle has traditionally been conceived as an independent hydrophobic layer that covers the external epidermal cell wall. Due to its complexity, the existing relationship between cuticle chemical composition and ultra-structure remains unclear to date. This study aimed to examine the link between chemical composition and structure of isolated, adaxial leaf cuticles of Eucalyptus camaldulensis and E. globulus by the gradual extraction and identification of lipid constituents (cutin and soluble lipids), coupled to spectroscopic and microscopic analyses. The soluble compounds and cutin monomers identified could not be assigned to a concrete internal cuticle ultra-structure. After cutin depolymerization, a cellulose network resembling the cell wall was observed, with different structural patterns in the regions ascribed to the cuticle proper and cuticular layer, respectively. Our results suggest that the current cuticle model should be revised, stressing the presence and major role of cell wall polysaccharides. It is concluded that the cuticle may be interpreted as a modified cell wall region which contains additional lipids. The major heterogeneity of the plant cuticle makes it difficult to establish a direct link between cuticle chemistry and structure with the existing methodologies. |
| first_indexed | 2024-04-14T05:49:16Z |
| format | Article |
| id | doaj.art-3413c70dd34f4b0dbc7a916b5bab2b11 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-14T05:49:16Z |
| publishDate | 2014-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3413c70dd34f4b0dbc7a916b5bab2b112022-12-22T02:09:11ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2014-09-01510.3389/fpls.2014.00481108706Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall regionPaula eGuzmán0Victoria eFernandez1José eGraça2Vanessa eCabral3Nour eKayali4Mohamed eKhayet5Luis eGil6Technical University of Madrid (UPM)Technical University of Madrid (UPM)Universidade de LisboaUniversidade de LisboaUniversidad Complutense de MadridUniversidad Complutense de MadridTechnical University of Madrid (UPM)The plant cuticle has traditionally been conceived as an independent hydrophobic layer that covers the external epidermal cell wall. Due to its complexity, the existing relationship between cuticle chemical composition and ultra-structure remains unclear to date. This study aimed to examine the link between chemical composition and structure of isolated, adaxial leaf cuticles of Eucalyptus camaldulensis and E. globulus by the gradual extraction and identification of lipid constituents (cutin and soluble lipids), coupled to spectroscopic and microscopic analyses. The soluble compounds and cutin monomers identified could not be assigned to a concrete internal cuticle ultra-structure. After cutin depolymerization, a cellulose network resembling the cell wall was observed, with different structural patterns in the regions ascribed to the cuticle proper and cuticular layer, respectively. Our results suggest that the current cuticle model should be revised, stressing the presence and major role of cell wall polysaccharides. It is concluded that the cuticle may be interpreted as a modified cell wall region which contains additional lipids. The major heterogeneity of the plant cuticle makes it difficult to establish a direct link between cuticle chemistry and structure with the existing methodologies.http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00481/fullCell WallLipidsPolysaccharidescuticleleafCutin |
| spellingShingle | Paula eGuzmán Victoria eFernandez José eGraça Vanessa eCabral Nour eKayali Mohamed eKhayet Luis eGil Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region Frontiers in Plant Science Cell Wall Lipids Polysaccharides cuticle leaf Cutin |
| title | Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region |
| title_full | Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region |
| title_fullStr | Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region |
| title_full_unstemmed | Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region |
| title_short | Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region |
| title_sort | chemical and structural analysis of eucalyptus globulus and e camaldulensis leaf cuticles a lipidized cell wall region |
| topic | Cell Wall Lipids Polysaccharides cuticle leaf Cutin |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00481/full |
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