Molecular Characterization of <i>Lipoptena</i> <i>fortisetosa</i> from Environmental Samples Collected in North-Eastern Poland
Recent years have witnessed an increase in the population of <i>Lipoptena</i><i>fortisetosa</i> in Central Europe. The genetic profile of this ectoparasite has not been studied in Poland to date. The aim of the present study was to confirm the presence of <i>L.</i>...
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MDPI AG
2021-04-01
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| author | Remigiusz Gałęcki Xuenan Xuan Tadeusz Bakuła Jerzy Jaroszewski |
| author_facet | Remigiusz Gałęcki Xuenan Xuan Tadeusz Bakuła Jerzy Jaroszewski |
| author_sort | Remigiusz Gałęcki |
| collection | DOAJ |
| description | Recent years have witnessed an increase in the population of <i>Lipoptena</i><i>fortisetosa</i> in Central Europe. The genetic profile of this ectoparasite has not been studied in Poland to date. The aim of the present study was to confirm the presence of <i>L.</i><i>fortisetosa</i> in north-eastern Poland and to characterize the examined population with the use of molecular methods. Deer keds were collected between June and July 2019 in six natural, mixed forests. A fragment of the rRNA 16S gene was used as a marker to identify <i>L.</i><i>fortisetosa</i> by polymerase chain reaction (PCR). DNA samples were sequenced in the last step. Six new locations of <i>L. fortisetosa</i> were confirmed. No significant differences were observed in the sex ratios of <i>L. cervi</i> and <i>L. fortisetosa</i> (<i>L. cervi p</i>-value = 0.74; <i>L. fortisetosa p</i>-value = 0.65). Significant differences were noted between the total size of <i>L. cervi</i> and <i>L. fortisetosa</i> populations (<i>p</i>-value < 0.001). The similarity to GenBank sequences ranged from 95.56% to 100%. The obtained nucleotide sequences were very closely related to <i>L. fortisetosa</i> sequences from Lithuania, the Czech Republic, and Japan. Molecular analyses revealed considerable genetic diversity, which could indicate that various ectoparasite lineages have spread throughout Europe. |
| first_indexed | 2024-03-10T12:24:54Z |
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| last_indexed | 2024-03-10T12:24:54Z |
| publishDate | 2021-04-01 |
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| series | Animals |
| spelling | doaj.art-3413f513677e49f58b267ef0b8b350f22023-11-21T15:08:04ZengMDPI AGAnimals2076-26152021-04-01114109310.3390/ani11041093Molecular Characterization of <i>Lipoptena</i> <i>fortisetosa</i> from Environmental Samples Collected in North-Eastern PolandRemigiusz Gałęcki0Xuenan Xuan1Tadeusz Bakuła2Jerzy Jaroszewski3Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, PolandNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanDepartment of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, PolandDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, PolandRecent years have witnessed an increase in the population of <i>Lipoptena</i><i>fortisetosa</i> in Central Europe. The genetic profile of this ectoparasite has not been studied in Poland to date. The aim of the present study was to confirm the presence of <i>L.</i><i>fortisetosa</i> in north-eastern Poland and to characterize the examined population with the use of molecular methods. Deer keds were collected between June and July 2019 in six natural, mixed forests. A fragment of the rRNA 16S gene was used as a marker to identify <i>L.</i><i>fortisetosa</i> by polymerase chain reaction (PCR). DNA samples were sequenced in the last step. Six new locations of <i>L. fortisetosa</i> were confirmed. No significant differences were observed in the sex ratios of <i>L. cervi</i> and <i>L. fortisetosa</i> (<i>L. cervi p</i>-value = 0.74; <i>L. fortisetosa p</i>-value = 0.65). Significant differences were noted between the total size of <i>L. cervi</i> and <i>L. fortisetosa</i> populations (<i>p</i>-value < 0.001). The similarity to GenBank sequences ranged from 95.56% to 100%. The obtained nucleotide sequences were very closely related to <i>L. fortisetosa</i> sequences from Lithuania, the Czech Republic, and Japan. Molecular analyses revealed considerable genetic diversity, which could indicate that various ectoparasite lineages have spread throughout Europe.https://www.mdpi.com/2076-2615/11/4/1093deer kedsectoparasiteHippoboscidaePCRphylogeneticsvector |
| spellingShingle | Remigiusz Gałęcki Xuenan Xuan Tadeusz Bakuła Jerzy Jaroszewski Molecular Characterization of <i>Lipoptena</i> <i>fortisetosa</i> from Environmental Samples Collected in North-Eastern Poland Animals deer keds ectoparasite Hippoboscidae PCR phylogenetics vector |
| title | Molecular Characterization of <i>Lipoptena</i> <i>fortisetosa</i> from Environmental Samples Collected in North-Eastern Poland |
| title_full | Molecular Characterization of <i>Lipoptena</i> <i>fortisetosa</i> from Environmental Samples Collected in North-Eastern Poland |
| title_fullStr | Molecular Characterization of <i>Lipoptena</i> <i>fortisetosa</i> from Environmental Samples Collected in North-Eastern Poland |
| title_full_unstemmed | Molecular Characterization of <i>Lipoptena</i> <i>fortisetosa</i> from Environmental Samples Collected in North-Eastern Poland |
| title_short | Molecular Characterization of <i>Lipoptena</i> <i>fortisetosa</i> from Environmental Samples Collected in North-Eastern Poland |
| title_sort | molecular characterization of i lipoptena i i fortisetosa i from environmental samples collected in north eastern poland |
| topic | deer keds ectoparasite Hippoboscidae PCR phylogenetics vector |
| url | https://www.mdpi.com/2076-2615/11/4/1093 |
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