The polymerase chain reaction-based SARS-CoV-2 detection
Introduction: the reverse transcriptase polymerase chain reaction is a highly precise technique in the detection and amplification of genetic material, and therefore in the detection of viruses. Objective: to describe the bases of the use of the polymerase chain reaction with reverse transcriptase...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidad de Ciencias Médicas de Pinar del Río
2020-08-01
|
| Series: | Universidad Médica Pinareña |
| Subjects: | |
| Online Access: | http://www.revgaleno.sld.cu/index.php/ump/article/view/574 |
| _version_ | 1827887171886907392 |
|---|---|
| author | José Francisco Cancino-Mesa Adrián Alejandro Vitón-Castillo Jorge Casí-Torres |
| author_facet | José Francisco Cancino-Mesa Adrián Alejandro Vitón-Castillo Jorge Casí-Torres |
| author_sort | José Francisco Cancino-Mesa |
| collection | DOAJ |
| description | Introduction: the reverse transcriptase polymerase chain reaction is a highly precise technique in the detection and amplification of genetic material, and therefore in the detection of viruses.
Objective: to describe the bases of the use of the polymerase chain reaction with reverse transcriptase as a diagnostic test in the detection of SARS-CoV-2.
Method: a literature review was carried out on articles published up to May 2020. The following databases were consulted: Scopus, Wiley Online Library, SciELO, DIALNET, EBSCO, MEDLINE and PubMed. Articles in Spanish and English were retrieved, selecting 43 references.
Development: The reverse transcriptase polymerase chain reaction to detect SARS-CoV-2 consists of reading RNA-dependent RNA polymerase, ORF1ab fragments, the E gene, the N gene, and the S gene. The nasopharyngeal exudate offers better results than oropharyngeal and saliva as sample. The inclusion of reverse transcriptase polymerase chain reaction tests using rectal swab specimens is necessary in suspected false negative cases. New studies and techniques are developed with the aim of optimizing the detection process.
Conclusions: the availability of diagnostic tests is crucial for the isolation of positive cases and the monitoring of the epidemiological chain of transmission. RT-PCR turned out to be the test of choice during the viral replication period. The RT-LAMP assay is a rapid diagnostic alternative with similar principles to RT-PCR. |
| first_indexed | 2024-03-12T20:13:25Z |
| format | Article |
| id | doaj.art-3415e300db4b4b8fb805173a45ad6e80 |
| institution | Directory Open Access Journal |
| issn | 1990-7990 |
| language | English |
| last_indexed | 2024-03-12T20:13:25Z |
| publishDate | 2020-08-01 |
| publisher | Universidad de Ciencias Médicas de Pinar del Río |
| record_format | Article |
| series | Universidad Médica Pinareña |
| spelling | doaj.art-3415e300db4b4b8fb805173a45ad6e802023-08-02T01:28:50ZengUniversidad de Ciencias Médicas de Pinar del RíoUniversidad Médica Pinareña1990-79902020-08-01171e574e574512The polymerase chain reaction-based SARS-CoV-2 detectionJosé Francisco Cancino-Mesa0Adrián Alejandro Vitón-Castillo1Jorge Casí-Torres2Universidad de Ciencias Médicas de Granma. Facultad de Ciencias Médicas “Celia Sánchez Manduley”. GranmaUniversidad de Ciencias Médicas de Pinar del Río. Facultad de Ciencias Médicas “Dr. Ernesto Guevara de la Serna”. Pinar del RíoUniversidad de Ciencias Médicas de Granma. Hospital Clínico Quirúrgico “Celia Sánchez Manduley”. GranmaIntroduction: the reverse transcriptase polymerase chain reaction is a highly precise technique in the detection and amplification of genetic material, and therefore in the detection of viruses. Objective: to describe the bases of the use of the polymerase chain reaction with reverse transcriptase as a diagnostic test in the detection of SARS-CoV-2. Method: a literature review was carried out on articles published up to May 2020. The following databases were consulted: Scopus, Wiley Online Library, SciELO, DIALNET, EBSCO, MEDLINE and PubMed. Articles in Spanish and English were retrieved, selecting 43 references. Development: The reverse transcriptase polymerase chain reaction to detect SARS-CoV-2 consists of reading RNA-dependent RNA polymerase, ORF1ab fragments, the E gene, the N gene, and the S gene. The nasopharyngeal exudate offers better results than oropharyngeal and saliva as sample. The inclusion of reverse transcriptase polymerase chain reaction tests using rectal swab specimens is necessary in suspected false negative cases. New studies and techniques are developed with the aim of optimizing the detection process. Conclusions: the availability of diagnostic tests is crucial for the isolation of positive cases and the monitoring of the epidemiological chain of transmission. RT-PCR turned out to be the test of choice during the viral replication period. The RT-LAMP assay is a rapid diagnostic alternative with similar principles to RT-PCR.http://www.revgaleno.sld.cu/index.php/ump/article/view/574infecciones por coronavirusreacción en cadena de la polimerasatécnicas de laboratorio clínico |
| spellingShingle | José Francisco Cancino-Mesa Adrián Alejandro Vitón-Castillo Jorge Casí-Torres The polymerase chain reaction-based SARS-CoV-2 detection Universidad Médica Pinareña infecciones por coronavirus reacción en cadena de la polimerasa técnicas de laboratorio clínico |
| title | The polymerase chain reaction-based SARS-CoV-2 detection |
| title_full | The polymerase chain reaction-based SARS-CoV-2 detection |
| title_fullStr | The polymerase chain reaction-based SARS-CoV-2 detection |
| title_full_unstemmed | The polymerase chain reaction-based SARS-CoV-2 detection |
| title_short | The polymerase chain reaction-based SARS-CoV-2 detection |
| title_sort | polymerase chain reaction based sars cov 2 detection |
| topic | infecciones por coronavirus reacción en cadena de la polimerasa técnicas de laboratorio clínico |
| url | http://www.revgaleno.sld.cu/index.php/ump/article/view/574 |
| work_keys_str_mv | AT josefranciscocancinomesa thepolymerasechainreactionbasedsarscov2detection AT adrianalejandrovitoncastillo thepolymerasechainreactionbasedsarscov2detection AT jorgecasitorres thepolymerasechainreactionbasedsarscov2detection AT josefranciscocancinomesa polymerasechainreactionbasedsarscov2detection AT adrianalejandrovitoncastillo polymerasechainreactionbasedsarscov2detection AT jorgecasitorres polymerasechainreactionbasedsarscov2detection |