Engineering a Circular Riboregulator in Escherichia coli
RNAs of different shapes and sizes, natural or synthetic, can regulate gene expression in prokaryotes and eukaryotes. Circular RNAs have recently appeared to be more widespread than previously thought, but their role in prokaryotes remains elusive. Here, by inserting a riboregulatory sequence within...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2020-01-01
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| Series: | BioDesign Research |
| Online Access: | http://dx.doi.org/10.34133/2020/1916789 |
| _version_ | 1827333995693604864 |
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| author | William Rostain Shensi Shen Teresa Cordero Guillermo Rodrigo Alfonso Jaramillo |
| author_facet | William Rostain Shensi Shen Teresa Cordero Guillermo Rodrigo Alfonso Jaramillo |
| author_sort | William Rostain |
| collection | DOAJ |
| description | RNAs of different shapes and sizes, natural or synthetic, can regulate gene expression in prokaryotes and eukaryotes. Circular RNAs have recently appeared to be more widespread than previously thought, but their role in prokaryotes remains elusive. Here, by inserting a riboregulatory sequence within a group I permuted intron-exon ribozyme, we created a small noncoding RNA that self-splices to produce a circular riboregulator in Escherichia coli. We showed that the resulting riboregulator can trans-activate gene expression by interacting with a cis-repressed messenger RNA. We characterized the system with a fluorescent reporter and with an antibiotic resistance marker, and we modeled this novel posttranscriptional mechanism. This first reported example of a circular RNA regulating gene expression in E. coli adds to an increasing repertoire of RNA synthetic biology parts, and it highlights that topological molecules can play a role in the case of prokaryotic regulation. |
| first_indexed | 2024-03-07T17:44:41Z |
| format | Article |
| id | doaj.art-3419720c30f04fcfbf4ce4dfac71658a |
| institution | Directory Open Access Journal |
| issn | 2693-1257 |
| language | English |
| last_indexed | 2024-03-07T17:44:41Z |
| publishDate | 2020-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | BioDesign Research |
| spelling | doaj.art-3419720c30f04fcfbf4ce4dfac71658a2024-03-02T15:19:05ZengAmerican Association for the Advancement of Science (AAAS)BioDesign Research2693-12572020-01-01202010.34133/2020/1916789Engineering a Circular Riboregulator in Escherichia coliWilliam Rostain0Shensi Shen1Teresa Cordero2Guillermo Rodrigo3Alfonso Jaramillo4Warwick Integrative Synthetic Biology Centre (WISB) and School of Life Sciences, University of Warwick, CV4 7AL Coventry, UK; Institute of Systems and Synthetic Biology, CNRS-Université d’Évry Val-d’Essonne, 91000 Évry, FranceInstitute of Systems and Synthetic Biology, CNRS-Université d’Évry Val-d’Essonne, 91000 Évry, FranceWarwick Integrative Synthetic Biology Centre (WISB) and School of Life Sciences, University of Warwick, CV4 7AL Coventry, UK; Instituto de Biología Molecular y Celular de Plantas, CSIC-Universidad Politécnica de Valencia, 46022 Valencia, SpainInstitute of Systems and Synthetic Biology, CNRS-Université d’Évry Val-d’Essonne, 91000 Évry, France; Instituto de Biología Molecular y Celular de Plantas, CSIC-Universidad Politécnica de Valencia, 46022 Valencia, Spain; Institute for Integrative Systems Biology (I2SysBio), CSIC-Universitat de València, 46980 Paterna, SpainWarwick Integrative Synthetic Biology Centre (WISB) and School of Life Sciences, University of Warwick, CV4 7AL Coventry, UK; Institute of Systems and Synthetic Biology, CNRS-Université d’Évry Val-d’Essonne, 91000 Évry, France; Institute for Integrative Systems Biology (I2SysBio), CSIC-Universitat de València, 46980 Paterna, SpainRNAs of different shapes and sizes, natural or synthetic, can regulate gene expression in prokaryotes and eukaryotes. Circular RNAs have recently appeared to be more widespread than previously thought, but their role in prokaryotes remains elusive. Here, by inserting a riboregulatory sequence within a group I permuted intron-exon ribozyme, we created a small noncoding RNA that self-splices to produce a circular riboregulator in Escherichia coli. We showed that the resulting riboregulator can trans-activate gene expression by interacting with a cis-repressed messenger RNA. We characterized the system with a fluorescent reporter and with an antibiotic resistance marker, and we modeled this novel posttranscriptional mechanism. This first reported example of a circular RNA regulating gene expression in E. coli adds to an increasing repertoire of RNA synthetic biology parts, and it highlights that topological molecules can play a role in the case of prokaryotic regulation.http://dx.doi.org/10.34133/2020/1916789 |
| spellingShingle | William Rostain Shensi Shen Teresa Cordero Guillermo Rodrigo Alfonso Jaramillo Engineering a Circular Riboregulator in Escherichia coli BioDesign Research |
| title | Engineering a Circular Riboregulator in Escherichia coli |
| title_full | Engineering a Circular Riboregulator in Escherichia coli |
| title_fullStr | Engineering a Circular Riboregulator in Escherichia coli |
| title_full_unstemmed | Engineering a Circular Riboregulator in Escherichia coli |
| title_short | Engineering a Circular Riboregulator in Escherichia coli |
| title_sort | engineering a circular riboregulator in escherichia coli |
| url | http://dx.doi.org/10.34133/2020/1916789 |
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