Genome-wide analysis and expression profiling of CalS genes in Glycine max revealed their role in development and salt stress
Abiotic stress affects plants' growth and development. Soybean is an important crop of the world, however, its production is affected by abiotic stresses. Callose Synthase is the most crucial enzyme response to environmental and developmental signals. However, in soybean, information on the cal...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364723005116 |
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| author | Madiha Zaynab Zhao-Shi Xu Hassan. A. Yad Athar Hussain Yasir Sharif Rashid Al-Yahyai Monther Sadder Abeer S Aloufi Shuangfei Li |
| author_facet | Madiha Zaynab Zhao-Shi Xu Hassan. A. Yad Athar Hussain Yasir Sharif Rashid Al-Yahyai Monther Sadder Abeer S Aloufi Shuangfei Li |
| author_sort | Madiha Zaynab |
| collection | DOAJ |
| description | Abiotic stress affects plants' growth and development. Soybean is an important crop of the world, however, its production is affected by abiotic stresses. Callose Synthase is the most crucial enzyme response to environmental and developmental signals. However, in soybean, information on the callose synthase genes is limited. In this study, we analyzed the callose synthase gene family of soybean at the genome-wide scale. We also studied the genes positions, gene structure, evolutionary relations, miRNAs target sites, and expression of CalS genes. Resultantly 24 CalS genes were found in soybean, with diverse chromosomal locations, cis-acting elements, conserved motifs, and gene structures. Further, GmCalS genes were divided into four phylogenetic classes. The evolutionary classification of CalSs was supported by the motif and gene structure analyses. Phytohormones, abiotic stresses, and growth-responsive elements were identified in the promoter of GmCalSs. In addition, the GmCalS genes higher expression in roots, leaves, flowers, and nodules tissues provided their significance in development. Furthermore, the higher expression of GmCalS17 and GmCalS19 genes in response to salt stress indicated their importance against salt stress. These findings will be helpful for further investigation of the CalS genes in other crops. |
| first_indexed | 2024-03-08T13:02:03Z |
| format | Article |
| id | doaj.art-341b407f2b8a4720b865e94c6f9f4674 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-03-08T13:02:03Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-341b407f2b8a4720b865e94c6f9f46742024-01-19T04:50:18ZengElsevierJournal of King Saud University: Science1018-36472024-02-01362103049Genome-wide analysis and expression profiling of CalS genes in Glycine max revealed their role in development and salt stressMadiha Zaynab0Zhao-Shi Xu1Hassan. A. Yad2Athar Hussain3Yasir Sharif4Rashid Al-Yahyai5Monther Sadder6Abeer S Aloufi7Shuangfei Li8Department of Biochemistry, Institute of Biological Sciences, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan 64200, Pakistan; Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Sciences, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong 51807, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/ National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, ChinaDepartment of Biological Sciences, Faculty of Science, Kuwait University, P.O. Box 5969, Safat 13060, KuwaitGenomics Lab, School of Food and Agricultural Sciences (SFAS), University of Management and Technology (UMT), Lahore, PakistanCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, ChinaDepartment of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, PO Box 34, Al-Khod 123, Muscat, OmanSchool of Agriculture, University of Jordan, Amman 11942, JordanDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaShenzhen Key Laboratory of Marine Bioresource & Eco-environmental Sciences, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong 51807, China; Corresponding author.Abiotic stress affects plants' growth and development. Soybean is an important crop of the world, however, its production is affected by abiotic stresses. Callose Synthase is the most crucial enzyme response to environmental and developmental signals. However, in soybean, information on the callose synthase genes is limited. In this study, we analyzed the callose synthase gene family of soybean at the genome-wide scale. We also studied the genes positions, gene structure, evolutionary relations, miRNAs target sites, and expression of CalS genes. Resultantly 24 CalS genes were found in soybean, with diverse chromosomal locations, cis-acting elements, conserved motifs, and gene structures. Further, GmCalS genes were divided into four phylogenetic classes. The evolutionary classification of CalSs was supported by the motif and gene structure analyses. Phytohormones, abiotic stresses, and growth-responsive elements were identified in the promoter of GmCalSs. In addition, the GmCalS genes higher expression in roots, leaves, flowers, and nodules tissues provided their significance in development. Furthermore, the higher expression of GmCalS17 and GmCalS19 genes in response to salt stress indicated their importance against salt stress. These findings will be helpful for further investigation of the CalS genes in other crops.http://www.sciencedirect.com/science/article/pii/S1018364723005116Callose SynthaseExpressionGrowthRegulationqRT-PCRSalt |
| spellingShingle | Madiha Zaynab Zhao-Shi Xu Hassan. A. Yad Athar Hussain Yasir Sharif Rashid Al-Yahyai Monther Sadder Abeer S Aloufi Shuangfei Li Genome-wide analysis and expression profiling of CalS genes in Glycine max revealed their role in development and salt stress Journal of King Saud University: Science Callose Synthase Expression Growth Regulation qRT-PCR Salt |
| title | Genome-wide analysis and expression profiling of CalS genes in Glycine max revealed their role in development and salt stress |
| title_full | Genome-wide analysis and expression profiling of CalS genes in Glycine max revealed their role in development and salt stress |
| title_fullStr | Genome-wide analysis and expression profiling of CalS genes in Glycine max revealed their role in development and salt stress |
| title_full_unstemmed | Genome-wide analysis and expression profiling of CalS genes in Glycine max revealed their role in development and salt stress |
| title_short | Genome-wide analysis and expression profiling of CalS genes in Glycine max revealed their role in development and salt stress |
| title_sort | genome wide analysis and expression profiling of cals genes in glycine max revealed their role in development and salt stress |
| topic | Callose Synthase Expression Growth Regulation qRT-PCR Salt |
| url | http://www.sciencedirect.com/science/article/pii/S1018364723005116 |
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