Separation and identification of carp pituitary proteins and glycoproteins
Carp pituitary proteins and glycoproteins were separated by the combination of immobilized metal affinity chromatography (IMAC) and affinity chromatography on Con A-Sepharose. The protein fractions were analysed by SDS-PAGE. Luteinizing hormone (in the α1β and α2β forms), growth hormone, free α1 sub...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2005-09-01
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| Series: | Czech Journal of Animal Science |
| Subjects: | |
| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-200509-0007_separation-and-identification-of-carp-pituitary-proteins-and-glycoproteins.php |
| _version_ | 1797899327415255040 |
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| author | H. Ryšlavá M. Janatová G. Čalounová I. Selicharová J. Barthová T. Barth |
| author_facet | H. Ryšlavá M. Janatová G. Čalounová I. Selicharová J. Barthová T. Barth |
| author_sort | H. Ryšlavá |
| collection | DOAJ |
| description | Carp pituitary proteins and glycoproteins were separated by the combination of immobilized metal affinity chromatography (IMAC) and affinity chromatography on Con A-Sepharose. The protein fractions were analysed by SDS-PAGE. Luteinizing hormone (in the α1β and α2β forms), growth hormone, free α1 subunit and β subunit of thyroid-stimulating hormone were identified by N-terminal amino acid sequencing. N-linked oligosaccharide chains of thyroid-stimulating hormone (β TSH) were separated after fluorescent labelling on a GlycoSep N column and treated by exoglycosidases. Among the saccharide components, complex and hybrid structures terminating SO4-GalNAc-GlcNAc-Man-, and high mannose structures, with 1 to 8 mannose units attached to the oligosaccharide core (GlcNAc)2(Man)3, were found. |
| first_indexed | 2024-04-10T08:28:11Z |
| format | Article |
| id | doaj.art-341b9e7775b149d79b82059971586914 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:28:11Z |
| publishDate | 2005-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-341b9e7775b149d79b820599715869142023-02-23T03:32:18ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092005-09-0150943043710.17221/4232-CJAScjs-200509-0007Separation and identification of carp pituitary proteins and glycoproteinsH. Ryšlavá0M. Janatová1G. Čalounová2I. Selicharová3J. Barthová4T. Barth5Department of Biochemistry, Faculty of Science, Charles University of Prague, Prague, Czech RepublicDepartment of Biochemistry, Faculty of Science, Charles University of Prague, Prague, Czech RepublicDepartment of Biochemistry, Faculty of Science, Charles University of Prague, Prague, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech RepublicDepartment of Biochemistry, Faculty of Science, Charles University of Prague, Prague, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech RepublicCarp pituitary proteins and glycoproteins were separated by the combination of immobilized metal affinity chromatography (IMAC) and affinity chromatography on Con A-Sepharose. The protein fractions were analysed by SDS-PAGE. Luteinizing hormone (in the α1β and α2β forms), growth hormone, free α1 subunit and β subunit of thyroid-stimulating hormone were identified by N-terminal amino acid sequencing. N-linked oligosaccharide chains of thyroid-stimulating hormone (β TSH) were separated after fluorescent labelling on a GlycoSep N column and treated by exoglycosidases. Among the saccharide components, complex and hybrid structures terminating SO4-GalNAc-GlcNAc-Man-, and high mannose structures, with 1 to 8 mannose units attached to the oligosaccharide core (GlcNAc)2(Man)3, were found.https://cjas.agriculturejournals.cz/artkey/cjs-200509-0007_separation-and-identification-of-carp-pituitary-proteins-and-glycoproteins.phpcarp hormonesglycoproteinsimmobilized metal ion affinity chromatography (imac)oligosaccharide chains |
| spellingShingle | H. Ryšlavá M. Janatová G. Čalounová I. Selicharová J. Barthová T. Barth Separation and identification of carp pituitary proteins and glycoproteins Czech Journal of Animal Science carp hormones glycoproteins immobilized metal ion affinity chromatography (imac) oligosaccharide chains |
| title | Separation and identification of carp pituitary proteins and glycoproteins |
| title_full | Separation and identification of carp pituitary proteins and glycoproteins |
| title_fullStr | Separation and identification of carp pituitary proteins and glycoproteins |
| title_full_unstemmed | Separation and identification of carp pituitary proteins and glycoproteins |
| title_short | Separation and identification of carp pituitary proteins and glycoproteins |
| title_sort | separation and identification of carp pituitary proteins and glycoproteins |
| topic | carp hormones glycoproteins immobilized metal ion affinity chromatography (imac) oligosaccharide chains |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-200509-0007_separation-and-identification-of-carp-pituitary-proteins-and-glycoproteins.php |
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