| Summary: | The objective of this research was to assess the effects of several irrigation solutions (ISOLs) on two major (P and Ca) and some minor (Fe, Zn, and Sr) elements of multi-root canals of radicular dentin in upper premolar teeth using X-ray fluorescence analysis (XRF). Powder of fifty radicular dentin specimens were prepared and divided into five groups based on treatments with different single and combined ISOLs. These groups were: G1-distilled water (control); G2- sodium hypochlorite (NaOCl, 5.25%); G3- sodium hypochlorite (NaOCl, 5.25%) and ethylendiaminetetraacetic acid (EDTA, 17%); G4- distilled water and a mixture of tetracycline, an acid, and a detergent (MTAD); and G5- sodium hypochlorite (NaOCl, 1.3%) and MTAD. The XRF results confirmed that the ISOL G5 (Ca/P=1.6254) was the most effective one. The ratio-to-ratio correlations revealed the common origins of Fe, Zn, and Sr in root canals of radicular dentin, which was highly interacted with the major elements of P and Ca each. These interactions were insignificantly affected by the used ISOLs. Finally, XRF is highly recommended as a safe, fast, nondestructive, and relatively not expensive technique to analyze the endodontic therapy of teeth.
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