Cocoa Bean Shell as Promising Feedstock for the Production of Poly(3-hydroxybutyrate) (PHB)
Cocoa bean shell (CBS), a by-product of the chocolate industry, has been employed as a substrate to obtain poly(3-hydroxybutyrate) (PHB) by fermentation with <i>Bacillus firmus</i>. With this aim, acid-thermal hydrolysis of CBS (20% <i>w</i>/<i>v</i>) was conducte...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/2/975 |
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| author | Marta Sánchez Amanda Laca Adriana Laca Mario Díaz |
| author_facet | Marta Sánchez Amanda Laca Adriana Laca Mario Díaz |
| author_sort | Marta Sánchez |
| collection | DOAJ |
| description | Cocoa bean shell (CBS), a by-product of the chocolate industry, has been employed as a substrate to obtain poly(3-hydroxybutyrate) (PHB) by fermentation with <i>Bacillus firmus</i>. With this aim, acid-thermal hydrolysis of CBS (20% <i>w</i>/<i>v</i>) was conducted at 135 °C for 10 min so that broths rich in fermentable sugars were obtained. These broths, both non-centrifuged and centrifuged, were employed as fermentation media. Significant polymer production was obtained from the broth with solids (non-centrifuged) with a yield of 107 mg of PHB/g dry matter. These results indicated that the presence of CBS solids played an important role in microorganism metabolism, with them being fundamental to the production of PHB. Experimental data were fitted by a model based on irreversible first-order reactions, and kinetic constants were obtained for solubilisation, hydrolysis, and sugar consumption. Although, several studies on obtaining PHB from other agri-food residues have been published, this is the first work on PHB production from CBS, with the study obtaining promising results with PHB concentrations similar or even higher than the others previously reported. |
| first_indexed | 2024-03-09T13:43:03Z |
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| id | doaj.art-342293f4a4c845718ac1f9d7a23cc269 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T13:43:03Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-342293f4a4c845718ac1f9d7a23cc2692023-11-30T21:04:23ZengMDPI AGApplied Sciences2076-34172023-01-0113297510.3390/app13020975Cocoa Bean Shell as Promising Feedstock for the Production of Poly(3-hydroxybutyrate) (PHB)Marta Sánchez0Amanda Laca1Adriana Laca2Mario Díaz3Department of Chemical and Environmental Engineering, University of Oviedo, 33006 Oviedo, SpainDepartment of Chemical and Environmental Engineering, University of Oviedo, 33006 Oviedo, SpainDepartment of Chemical and Environmental Engineering, University of Oviedo, 33006 Oviedo, SpainDepartment of Chemical and Environmental Engineering, University of Oviedo, 33006 Oviedo, SpainCocoa bean shell (CBS), a by-product of the chocolate industry, has been employed as a substrate to obtain poly(3-hydroxybutyrate) (PHB) by fermentation with <i>Bacillus firmus</i>. With this aim, acid-thermal hydrolysis of CBS (20% <i>w</i>/<i>v</i>) was conducted at 135 °C for 10 min so that broths rich in fermentable sugars were obtained. These broths, both non-centrifuged and centrifuged, were employed as fermentation media. Significant polymer production was obtained from the broth with solids (non-centrifuged) with a yield of 107 mg of PHB/g dry matter. These results indicated that the presence of CBS solids played an important role in microorganism metabolism, with them being fundamental to the production of PHB. Experimental data were fitted by a model based on irreversible first-order reactions, and kinetic constants were obtained for solubilisation, hydrolysis, and sugar consumption. Although, several studies on obtaining PHB from other agri-food residues have been published, this is the first work on PHB production from CBS, with the study obtaining promising results with PHB concentrations similar or even higher than the others previously reported.https://www.mdpi.com/2076-3417/13/2/975cocoa bean shellbioplasticspolyhydroxybutyratefermentable sugarshydrothermal treatment |
| spellingShingle | Marta Sánchez Amanda Laca Adriana Laca Mario Díaz Cocoa Bean Shell as Promising Feedstock for the Production of Poly(3-hydroxybutyrate) (PHB) Applied Sciences cocoa bean shell bioplastics polyhydroxybutyrate fermentable sugars hydrothermal treatment |
| title | Cocoa Bean Shell as Promising Feedstock for the Production of Poly(3-hydroxybutyrate) (PHB) |
| title_full | Cocoa Bean Shell as Promising Feedstock for the Production of Poly(3-hydroxybutyrate) (PHB) |
| title_fullStr | Cocoa Bean Shell as Promising Feedstock for the Production of Poly(3-hydroxybutyrate) (PHB) |
| title_full_unstemmed | Cocoa Bean Shell as Promising Feedstock for the Production of Poly(3-hydroxybutyrate) (PHB) |
| title_short | Cocoa Bean Shell as Promising Feedstock for the Production of Poly(3-hydroxybutyrate) (PHB) |
| title_sort | cocoa bean shell as promising feedstock for the production of poly 3 hydroxybutyrate phb |
| topic | cocoa bean shell bioplastics polyhydroxybutyrate fermentable sugars hydrothermal treatment |
| url | https://www.mdpi.com/2076-3417/13/2/975 |
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