Effect of Nano-Capsules Containing Risedronate on Calvarial Bone Formation in Rabbit: Radiography and Biochemical Investigation
Objective: The main purpose of the current study was to determine the effect of nano-capsules containing risedronate on calvarial bone formation in rabbit. Materials and Methods: Fifteen adult rabbits were allocated to the study. Four holes were created in the calvarial bone. Holes 1-4 were filled...
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| Format: | Article |
| Language: | English |
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Aras Part Medical International Press
2018-01-01
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| Series: | Crescent Journal of Medical and Biological Sciences |
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| Online Access: | http://cjmb.org/uploads/pdf/pdf_CJMB_167.pdf |
| _version_ | 1811248181090975744 |
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| author | Hesam Aldin Hoseinzadeh Ahmad Asghari Gholamreza Abedi Abolfazl Akbarzadeh Reza Sedaghat |
| author_facet | Hesam Aldin Hoseinzadeh Ahmad Asghari Gholamreza Abedi Abolfazl Akbarzadeh Reza Sedaghat |
| author_sort | Hesam Aldin Hoseinzadeh |
| collection | DOAJ |
| description | Objective: The main purpose of the current study was to determine the effect of nano-capsules containing risedronate on calvarial bone formation in rabbit.
Materials and Methods: Fifteen adult rabbits were allocated to the study. Four holes were created in the calvarial bone. Holes 1-4 were filled as described below: hole 1 was right unfilled and kept as control; hole 2 was filled with nano bone; hole 3 was filled using an autogenous bone; and hole 4 was filled with a mixture of nano-capsules containing risedronate. At 4, 8 and 12 weeks after surgery, blood samples were obtained and red blood cell, white blood cell, hemoglobin, hematocrit, mean cell volume, mean cell HGB and platelet counts were determined. Then animals were scarified and bone density was determined using radiography images.
Results: Bone formation in nano risedronate + autograft and autograft were 0.31 ± 0.03 and 0.25 ± 0.02, respectively, while in nano risedronate and control groups were 0.11 ± 0.01 and 0.08 ± 0.02, respectively (P < 0.05). Significant differences were observed in bone density in nano risedronate + autograft as 0.37 ± 0.01, while the density in the autograft, nano risedronate, and control groups were as 0.32 ± 0.01, 0.14 ± 0.01, and 0.09 ± 0.02, respectively (P < 0.05). Bone density after 12 weeks were 0.51 ± 0.01, 0.36 ± 0.02, 0.21±0.01, and 0.19 ± 0.02 in nano risedronate + autograft, nano risedronate, autograft, and control groups, respectively (P < 0.05). In all stages of the study, the nano risedronate + autograft group had better bone formation in comparison to the other groups (P < 0.05). Different filling materials of defects had no effect on blood hematology indexes (P > 0.05).
Conclusion: These results suggest nano risedronate + autograft has positive effects on calvarial bone defects healing in rabbit. |
| first_indexed | 2024-04-12T15:22:32Z |
| format | Article |
| id | doaj.art-3425297c9cba4318b33e841a048e055f |
| institution | Directory Open Access Journal |
| issn | 2148-9696 2148-9696 |
| language | English |
| last_indexed | 2024-04-12T15:22:32Z |
| publishDate | 2018-01-01 |
| publisher | Aras Part Medical International Press |
| record_format | Article |
| series | Crescent Journal of Medical and Biological Sciences |
| spelling | doaj.art-3425297c9cba4318b33e841a048e055f2022-12-22T03:27:22ZengAras Part Medical International PressCrescent Journal of Medical and Biological Sciences2148-96962148-96962018-01-01512933Effect of Nano-Capsules Containing Risedronate on Calvarial Bone Formation in Rabbit: Radiography and Biochemical InvestigationHesam Aldin Hoseinzadeh0Ahmad Asghari1Gholamreza Abedi2Abolfazl Akbarzadeh3 Reza Sedaghat4Department of Veterinary Surgery, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Veterinary Surgery, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Veterinary Surgery, Science and Research Branch, Islamic Azad University, Tehran, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Anatomy and Pathology, Faculty of Medicine, Shahed University, Tehran, Iran.Objective: The main purpose of the current study was to determine the effect of nano-capsules containing risedronate on calvarial bone formation in rabbit. Materials and Methods: Fifteen adult rabbits were allocated to the study. Four holes were created in the calvarial bone. Holes 1-4 were filled as described below: hole 1 was right unfilled and kept as control; hole 2 was filled with nano bone; hole 3 was filled using an autogenous bone; and hole 4 was filled with a mixture of nano-capsules containing risedronate. At 4, 8 and 12 weeks after surgery, blood samples were obtained and red blood cell, white blood cell, hemoglobin, hematocrit, mean cell volume, mean cell HGB and platelet counts were determined. Then animals were scarified and bone density was determined using radiography images. Results: Bone formation in nano risedronate + autograft and autograft were 0.31 ± 0.03 and 0.25 ± 0.02, respectively, while in nano risedronate and control groups were 0.11 ± 0.01 and 0.08 ± 0.02, respectively (P < 0.05). Significant differences were observed in bone density in nano risedronate + autograft as 0.37 ± 0.01, while the density in the autograft, nano risedronate, and control groups were as 0.32 ± 0.01, 0.14 ± 0.01, and 0.09 ± 0.02, respectively (P < 0.05). Bone density after 12 weeks were 0.51 ± 0.01, 0.36 ± 0.02, 0.21±0.01, and 0.19 ± 0.02 in nano risedronate + autograft, nano risedronate, autograft, and control groups, respectively (P < 0.05). In all stages of the study, the nano risedronate + autograft group had better bone formation in comparison to the other groups (P < 0.05). Different filling materials of defects had no effect on blood hematology indexes (P > 0.05). Conclusion: These results suggest nano risedronate + autograft has positive effects on calvarial bone defects healing in rabbit.http://cjmb.org/uploads/pdf/pdf_CJMB_167.pdfNano-capsulesRisedronateCalvarial healingRadiographyRabbit |
| spellingShingle | Hesam Aldin Hoseinzadeh Ahmad Asghari Gholamreza Abedi Abolfazl Akbarzadeh Reza Sedaghat Effect of Nano-Capsules Containing Risedronate on Calvarial Bone Formation in Rabbit: Radiography and Biochemical Investigation Crescent Journal of Medical and Biological Sciences Nano-capsules Risedronate Calvarial healing Radiography Rabbit |
| title | Effect of Nano-Capsules Containing Risedronate on Calvarial Bone Formation in Rabbit: Radiography and Biochemical Investigation |
| title_full | Effect of Nano-Capsules Containing Risedronate on Calvarial Bone Formation in Rabbit: Radiography and Biochemical Investigation |
| title_fullStr | Effect of Nano-Capsules Containing Risedronate on Calvarial Bone Formation in Rabbit: Radiography and Biochemical Investigation |
| title_full_unstemmed | Effect of Nano-Capsules Containing Risedronate on Calvarial Bone Formation in Rabbit: Radiography and Biochemical Investigation |
| title_short | Effect of Nano-Capsules Containing Risedronate on Calvarial Bone Formation in Rabbit: Radiography and Biochemical Investigation |
| title_sort | effect of nano capsules containing risedronate on calvarial bone formation in rabbit radiography and biochemical investigation |
| topic | Nano-capsules Risedronate Calvarial healing Radiography Rabbit |
| url | http://cjmb.org/uploads/pdf/pdf_CJMB_167.pdf |
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