Search for Mycobacterium leprae in wild mammals
Leprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, partic...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010-01-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867010700106 |
| _version_ | 1818555863534665728 |
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| author | Sílvia Cristina Barboza Pedrini, MD Patrícia Sammarco Rosa, MD Ísis Meri Medri, MD Guilherme Mourão, MD Eduardo Bagagli Carlos Alberto de Magalhães Lopes |
| author_facet | Sílvia Cristina Barboza Pedrini, MD Patrícia Sammarco Rosa, MD Ísis Meri Medri, MD Guilherme Mourão, MD Eduardo Bagagli Carlos Alberto de Magalhães Lopes |
| author_sort | Sílvia Cristina Barboza Pedrini, MD |
| collection | DOAJ |
| description | Leprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus sexcinctus, Cabassous unicinctus, and C. tatouay armadillos from the Middle Western region of the state of São Paulo and from the experimental station of Embrapa Pantanal, located in Pantanal da Nhecolândia of Mato Grosso do Sul state. Also, the molecular analysis of 19 samples from internal organs of other road killed species of wild animals, such as Nasua nasua (ring-tailed coati), Procyon cancrivoros (hand-skinned), Cerdocyon thous (dog-pity-bush), Cavia aperea (restless cavy), Didelphis albiventris (skunk), Sphigurrus spinosus (hedgehog), and Gallictis vittata (ferret) showed PCR negative data. None of the 157 analyzed samples had shown natural mycobacterial infection. Only the armadillo inoculated with material collected from untreated multibacillary leprosy patient presented PCR positive and its genomic sequencing revealed 100% identity with M. leprae. According to these preliminary studies, based on the used methodology, it is possible to conclude that wild mammals seem not to play an important role in the epidemiology of leprosy in the Middle Western region of the São Paulo state and in the Pantanal of Mato Grosso do Sul state. Keywords: Dasypus novemcinctus, Euphractus sexcinctus, Cabassous tatouay, Mycobacterium leprae, eco-epidemiology, wild mammals |
| first_indexed | 2024-12-12T12:59:01Z |
| format | Article |
| id | doaj.art-34280692841c4edf8dd520bc738feef5 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-12T12:59:01Z |
| publishDate | 2010-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-34280692841c4edf8dd520bc738feef52022-12-22T00:23:50ZengElsevierBrazilian Journal of Infectious Diseases1413-86702010-01-011414753Search for Mycobacterium leprae in wild mammalsSílvia Cristina Barboza Pedrini, MD0Patrícia Sammarco Rosa, MD1Ísis Meri Medri, MD2Guilherme Mourão, MD3Eduardo Bagagli4Carlos Alberto de Magalhães Lopes5Instituto Lauro de Souza Lima, Bauru – São Paulo, Brazil; Correspondence to: Sílvia Cristina Barboza Pedrini Rodovia Comandante, João Ribeiro de Barros, km 225/226 PO Box 3021 Bauru, SP, Brasil CEP: 17034-971.Instituto Lauro de Souza Lima, Bauru – São Paulo, BrazilEmbrapa Pantanal - Corumbá, MS, BrazilEmbrapa Pantanal - Corumbá, MS, BrazilMicrobiology and Immunology Department, Instituto de Biociências, Universidade Estadual Paulista (UNESP) - Botucatu, São Paulo, BrazilMicrobiology and Immunology Department, Instituto de Biociências, Universidade Estadual Paulista (UNESP) - Botucatu, São Paulo, BrazilLeprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus sexcinctus, Cabassous unicinctus, and C. tatouay armadillos from the Middle Western region of the state of São Paulo and from the experimental station of Embrapa Pantanal, located in Pantanal da Nhecolândia of Mato Grosso do Sul state. Also, the molecular analysis of 19 samples from internal organs of other road killed species of wild animals, such as Nasua nasua (ring-tailed coati), Procyon cancrivoros (hand-skinned), Cerdocyon thous (dog-pity-bush), Cavia aperea (restless cavy), Didelphis albiventris (skunk), Sphigurrus spinosus (hedgehog), and Gallictis vittata (ferret) showed PCR negative data. None of the 157 analyzed samples had shown natural mycobacterial infection. Only the armadillo inoculated with material collected from untreated multibacillary leprosy patient presented PCR positive and its genomic sequencing revealed 100% identity with M. leprae. According to these preliminary studies, based on the used methodology, it is possible to conclude that wild mammals seem not to play an important role in the epidemiology of leprosy in the Middle Western region of the São Paulo state and in the Pantanal of Mato Grosso do Sul state. Keywords: Dasypus novemcinctus, Euphractus sexcinctus, Cabassous tatouay, Mycobacterium leprae, eco-epidemiology, wild mammalshttp://www.sciencedirect.com/science/article/pii/S1413867010700106 |
| spellingShingle | Sílvia Cristina Barboza Pedrini, MD Patrícia Sammarco Rosa, MD Ísis Meri Medri, MD Guilherme Mourão, MD Eduardo Bagagli Carlos Alberto de Magalhães Lopes Search for Mycobacterium leprae in wild mammals Brazilian Journal of Infectious Diseases |
| title | Search for Mycobacterium leprae in wild mammals |
| title_full | Search for Mycobacterium leprae in wild mammals |
| title_fullStr | Search for Mycobacterium leprae in wild mammals |
| title_full_unstemmed | Search for Mycobacterium leprae in wild mammals |
| title_short | Search for Mycobacterium leprae in wild mammals |
| title_sort | search for mycobacterium leprae in wild mammals |
| url | http://www.sciencedirect.com/science/article/pii/S1413867010700106 |
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