The evaluation of the utility of the GENECUBE HQ SARS-CoV-2 for anterior nasal samples and saliva samples with a new rapid examination protocol
<h4>Introduction</h4> GENECUBE® is a rapid molecular identification system, and previous studies demonstrated that GENECUBE® HQ SARS-CoV-2 showed excellent analytical performance for the detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) with nasopharyngeal samples...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719657/?tool=EBI |
| _version_ | 1798034097888559104 |
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| author | Asami Naito Yoshihiko Kiyasu Yusaku Akashi Akio Sugiyama Masashi Michibuchi Yuto Takeuchi Shigeyuki Notake Koji Nakamura Hiroichi Ishikawa Hiromichi Suzuki |
| author_facet | Asami Naito Yoshihiko Kiyasu Yusaku Akashi Akio Sugiyama Masashi Michibuchi Yuto Takeuchi Shigeyuki Notake Koji Nakamura Hiroichi Ishikawa Hiromichi Suzuki |
| author_sort | Asami Naito |
| collection | DOAJ |
| description | <h4>Introduction</h4> GENECUBE® is a rapid molecular identification system, and previous studies demonstrated that GENECUBE® HQ SARS-CoV-2 showed excellent analytical performance for the detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) with nasopharyngeal samples. However, other respiratory samples have not been evaluated. <h4>Methods</h4> This prospective comparison between GENECUBE® HQ SARS-CoV-2 and reference real-time reverse transcriptase polymerase chain reaction (RT-PCR) was performed for the detection of SARS-CoV-2 using anterior nasal samples and saliva samples. Additionally, we evaluated a new rapid examination protocol using GENECUBE® HQ SARS-CoV-2 for the detection of SARS-CoV-2 with saliva samples. For the rapid protocol, in the preparation of saliva samples, purification and extraction processes were adjusted, and the total process time was shortened to approximately 35 minutes. <h4>Results</h4> For 359 anterior nasal samples, the total-, positive-, and negative concordance of the two assays was 99.7% (358/359), 98.1% (51/52), and 100% (307/307), respectively. For saliva samples, the total-, positive-, and negative concordance of the two assays was 99.6% (239/240), 100% (56/56), and 99.5% (183/184), respectively. With the new protocol, total-, positive-, and negative concordance of the two assays was 98.8% (237/240), 100% (56/56), and 98.4% (181/184), respectively. In all discordance cases, SARS-CoV-2 was detected by additional molecular examinations. <h4>Conclusion</h4> GENECUBE® HQ SARS-CoV-2 provided high analytical performance for the detection of SARS-CoV-2 in anterior nasal samples and saliva samples. |
| first_indexed | 2024-04-11T20:39:38Z |
| format | Article |
| id | doaj.art-3429614f13df4d68b83ddf909a3f427f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T20:39:38Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3429614f13df4d68b83ddf909a3f427f2022-12-22T04:04:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011612The evaluation of the utility of the GENECUBE HQ SARS-CoV-2 for anterior nasal samples and saliva samples with a new rapid examination protocolAsami NaitoYoshihiko KiyasuYusaku AkashiAkio SugiyamaMasashi MichibuchiYuto TakeuchiShigeyuki NotakeKoji NakamuraHiroichi IshikawaHiromichi Suzuki<h4>Introduction</h4> GENECUBE® is a rapid molecular identification system, and previous studies demonstrated that GENECUBE® HQ SARS-CoV-2 showed excellent analytical performance for the detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) with nasopharyngeal samples. However, other respiratory samples have not been evaluated. <h4>Methods</h4> This prospective comparison between GENECUBE® HQ SARS-CoV-2 and reference real-time reverse transcriptase polymerase chain reaction (RT-PCR) was performed for the detection of SARS-CoV-2 using anterior nasal samples and saliva samples. Additionally, we evaluated a new rapid examination protocol using GENECUBE® HQ SARS-CoV-2 for the detection of SARS-CoV-2 with saliva samples. For the rapid protocol, in the preparation of saliva samples, purification and extraction processes were adjusted, and the total process time was shortened to approximately 35 minutes. <h4>Results</h4> For 359 anterior nasal samples, the total-, positive-, and negative concordance of the two assays was 99.7% (358/359), 98.1% (51/52), and 100% (307/307), respectively. For saliva samples, the total-, positive-, and negative concordance of the two assays was 99.6% (239/240), 100% (56/56), and 99.5% (183/184), respectively. With the new protocol, total-, positive-, and negative concordance of the two assays was 98.8% (237/240), 100% (56/56), and 98.4% (181/184), respectively. In all discordance cases, SARS-CoV-2 was detected by additional molecular examinations. <h4>Conclusion</h4> GENECUBE® HQ SARS-CoV-2 provided high analytical performance for the detection of SARS-CoV-2 in anterior nasal samples and saliva samples.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719657/?tool=EBI |
| spellingShingle | Asami Naito Yoshihiko Kiyasu Yusaku Akashi Akio Sugiyama Masashi Michibuchi Yuto Takeuchi Shigeyuki Notake Koji Nakamura Hiroichi Ishikawa Hiromichi Suzuki The evaluation of the utility of the GENECUBE HQ SARS-CoV-2 for anterior nasal samples and saliva samples with a new rapid examination protocol PLoS ONE |
| title | The evaluation of the utility of the GENECUBE HQ SARS-CoV-2 for anterior nasal samples and saliva samples with a new rapid examination protocol |
| title_full | The evaluation of the utility of the GENECUBE HQ SARS-CoV-2 for anterior nasal samples and saliva samples with a new rapid examination protocol |
| title_fullStr | The evaluation of the utility of the GENECUBE HQ SARS-CoV-2 for anterior nasal samples and saliva samples with a new rapid examination protocol |
| title_full_unstemmed | The evaluation of the utility of the GENECUBE HQ SARS-CoV-2 for anterior nasal samples and saliva samples with a new rapid examination protocol |
| title_short | The evaluation of the utility of the GENECUBE HQ SARS-CoV-2 for anterior nasal samples and saliva samples with a new rapid examination protocol |
| title_sort | evaluation of the utility of the genecube hq sars cov 2 for anterior nasal samples and saliva samples with a new rapid examination protocol |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719657/?tool=EBI |
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