High-throughput screening of ancient forest plant extracts shows cytotoxicity towards triple-negative breast cancer
According to the World Health Organization, women's breast cancer is among the most common cancers with 7.8 million diagnosed cases during 2016–2020 and encompasses 15 % of all female cancer-related mortalities. These mortality events from triple-negative breast cancer are a significant health...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Environment International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412023005524 |
| _version_ | 1797615981345898496 |
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| author | Yiyang Li Nyuk Ling Ma Huiling Chen Jiateng Zhong Dangquan Zhang Wanxi Peng Su Shiung Lam Yafeng Yang Xiaochen Yue Lijun Yan Ting Wang Bjarne Styrishave Tomasz Maciej Ciesielski Christian Sonne |
| author_facet | Yiyang Li Nyuk Ling Ma Huiling Chen Jiateng Zhong Dangquan Zhang Wanxi Peng Su Shiung Lam Yafeng Yang Xiaochen Yue Lijun Yan Ting Wang Bjarne Styrishave Tomasz Maciej Ciesielski Christian Sonne |
| author_sort | Yiyang Li |
| collection | DOAJ |
| description | According to the World Health Organization, women's breast cancer is among the most common cancers with 7.8 million diagnosed cases during 2016–2020 and encompasses 15 % of all female cancer-related mortalities. These mortality events from triple-negative breast cancer are a significant health issue worldwide calling for a continuous search of bioactive compounds for better cancer treatments. Historically, plants are important sources for identifying such new bioactive chemicals for treatments. Here we use high-throughput screening and mass spectrometry analyses of extracts from 100 plant species collected in Chinese ancient forests to detect novel bioactive breast cancer phytochemicals. First, to study the effects on viability of the plant extracts, we used a MTT and CCK-8 cytotoxicity assay employing triple-negative breast cancer (TNBC) MDA-MB-231 and normal epithelial MCF-10A cell lines and cell cycle arrest to estimate apoptosis using flow cytometry for the most potent three speices. Based on these analyses, the final most potent extracts were from the Amur honeysuckle (Lonicera maackii) wood/root bark and Nigaki (Picrasma quassioides) wood/root bark. Then, 5 × 106 MDA-MB-231 cells were injected subcutaneously into the right hind leg of nude mice and a tumour was allowed to grow before treatment for seven days. Subsequently, the four exposed groups received gavage extracts from Amur honeysuckle and Nigaki (Amur honeysuckle wood distilled water, Amur honeysuckle root bark ethanol, Nigaki wood ethanol or Nigaki root bark distilled water/ethanol (1:1) extracts) in phosphate-buffered saline (PBS), while the control group received only PBS. The tumour weight of treated nude mice was reduced significantly by 60.5 % within 2 weeks, while on average killing 70 % of the MDA-MB-231 breast cancer cells after 48 h treatment (MTT test). In addition, screening of target genes using the Swiss Target Prediction, STITCH, STRING and NCBI-gene database showed that the four plant extracts possess desirable activity towards several known breast cancer genes. This reflects that the extracts may kill MBD-MB-231 breast cancer cells. This is the first screening of plant extracts with high efficiency in 2 decades, showing promising results for future development of novel cancer treatments. |
| first_indexed | 2024-03-11T07:34:44Z |
| format | Article |
| id | doaj.art-342c7212c6aa499c8da5454b9cbb3cef |
| institution | Directory Open Access Journal |
| issn | 0160-4120 |
| language | English |
| last_indexed | 2024-03-11T07:34:44Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj.art-342c7212c6aa499c8da5454b9cbb3cef2023-11-17T05:24:51ZengElsevierEnvironment International0160-41202023-11-01181108279High-throughput screening of ancient forest plant extracts shows cytotoxicity towards triple-negative breast cancerYiyang Li0Nyuk Ling Ma1Huiling Chen2Jiateng Zhong3Dangquan Zhang4Wanxi Peng5Su Shiung Lam6Yafeng Yang7Xiaochen Yue8Lijun Yan9Ting Wang10Bjarne Styrishave11Tomasz Maciej Ciesielski12Christian Sonne13Henan Province International Collaboration Lab of Forest Resources Utilization, School of Forestry, Henan Agricultural University, Zhengzhou 450002, ChinaBIOSES Research Interest Group, Faculty of Science & Marine Environment, 21030 Universiti Malaysia Terengganu, Malaysia; Center for Global Health Research (CGHR), Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, IndiaHenan Province International Collaboration Lab of Forest Resources Utilization, School of Forestry, Henan Agricultural University, Zhengzhou 450002, ChinaDepartment of Pathology, Xinxiang Medical University, Xinxiang, ChinaHenan Province International Collaboration Lab of Forest Resources Utilization, School of Forestry, Henan Agricultural University, Zhengzhou 450002, ChinaHenan Province International Collaboration Lab of Forest Resources Utilization, School of Forestry, Henan Agricultural University, Zhengzhou 450002, ChinaHigher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia; Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan, TaiwanHenan Province International Collaboration Lab of Forest Resources Utilization, School of Forestry, Henan Agricultural University, Zhengzhou 450002, ChinaHenan Province International Collaboration Lab of Forest Resources Utilization, School of Forestry, Henan Agricultural University, Zhengzhou 450002, ChinaHenan Province International Collaboration Lab of Forest Resources Utilization, School of Forestry, Henan Agricultural University, Zhengzhou 450002, ChinaHenan Province International Collaboration Lab of Forest Resources Utilization, School of Forestry, Henan Agricultural University, Zhengzhou 450002, ChinaToxicology and Drug Metabolism Group, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 3, DK-2100 Copenhagen, DenmarkDepartment of Biology, Norwegian University of Science and Technology, Høgskoleringen 5, NO-7491 Trondheim, NorwayDepartment of Ecoscience, Arctic Research Centre (ARC), Aarhus University, Frederiksborgvej 399, PO Box 358, DK-4000 Roskilde, Denmark; Sustainability Cluster, School of Engineering, University of Petroleum & Energy Studies, Dehradun, Uttarakhand 248007, India; Corresponding author at: Department of Ecoscience, Arctic Research Centre (ARC), Aarhus University, Frederiksborgvej 399, PO Box 358, DK-4000 Roskilde, Denmark.According to the World Health Organization, women's breast cancer is among the most common cancers with 7.8 million diagnosed cases during 2016–2020 and encompasses 15 % of all female cancer-related mortalities. These mortality events from triple-negative breast cancer are a significant health issue worldwide calling for a continuous search of bioactive compounds for better cancer treatments. Historically, plants are important sources for identifying such new bioactive chemicals for treatments. Here we use high-throughput screening and mass spectrometry analyses of extracts from 100 plant species collected in Chinese ancient forests to detect novel bioactive breast cancer phytochemicals. First, to study the effects on viability of the plant extracts, we used a MTT and CCK-8 cytotoxicity assay employing triple-negative breast cancer (TNBC) MDA-MB-231 and normal epithelial MCF-10A cell lines and cell cycle arrest to estimate apoptosis using flow cytometry for the most potent three speices. Based on these analyses, the final most potent extracts were from the Amur honeysuckle (Lonicera maackii) wood/root bark and Nigaki (Picrasma quassioides) wood/root bark. Then, 5 × 106 MDA-MB-231 cells were injected subcutaneously into the right hind leg of nude mice and a tumour was allowed to grow before treatment for seven days. Subsequently, the four exposed groups received gavage extracts from Amur honeysuckle and Nigaki (Amur honeysuckle wood distilled water, Amur honeysuckle root bark ethanol, Nigaki wood ethanol or Nigaki root bark distilled water/ethanol (1:1) extracts) in phosphate-buffered saline (PBS), while the control group received only PBS. The tumour weight of treated nude mice was reduced significantly by 60.5 % within 2 weeks, while on average killing 70 % of the MDA-MB-231 breast cancer cells after 48 h treatment (MTT test). In addition, screening of target genes using the Swiss Target Prediction, STITCH, STRING and NCBI-gene database showed that the four plant extracts possess desirable activity towards several known breast cancer genes. This reflects that the extracts may kill MBD-MB-231 breast cancer cells. This is the first screening of plant extracts with high efficiency in 2 decades, showing promising results for future development of novel cancer treatments.http://www.sciencedirect.com/science/article/pii/S0160412023005524GC-MSLC-QTOF-MSPhytochemicalsApoptosisGlobal goalsPlanetary health |
| spellingShingle | Yiyang Li Nyuk Ling Ma Huiling Chen Jiateng Zhong Dangquan Zhang Wanxi Peng Su Shiung Lam Yafeng Yang Xiaochen Yue Lijun Yan Ting Wang Bjarne Styrishave Tomasz Maciej Ciesielski Christian Sonne High-throughput screening of ancient forest plant extracts shows cytotoxicity towards triple-negative breast cancer Environment International GC-MS LC-QTOF-MS Phytochemicals Apoptosis Global goals Planetary health |
| title | High-throughput screening of ancient forest plant extracts shows cytotoxicity towards triple-negative breast cancer |
| title_full | High-throughput screening of ancient forest plant extracts shows cytotoxicity towards triple-negative breast cancer |
| title_fullStr | High-throughput screening of ancient forest plant extracts shows cytotoxicity towards triple-negative breast cancer |
| title_full_unstemmed | High-throughput screening of ancient forest plant extracts shows cytotoxicity towards triple-negative breast cancer |
| title_short | High-throughput screening of ancient forest plant extracts shows cytotoxicity towards triple-negative breast cancer |
| title_sort | high throughput screening of ancient forest plant extracts shows cytotoxicity towards triple negative breast cancer |
| topic | GC-MS LC-QTOF-MS Phytochemicals Apoptosis Global goals Planetary health |
| url | http://www.sciencedirect.com/science/article/pii/S0160412023005524 |
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