E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis
Introduction: Evidence suggests that e-cigarette use (vaping) increases cardiovascular disease risk, but decades are needed before people who vape would develop pathology. Thus, murine models of atherosclerosis can be utilized as tools to understand disease susceptibility, risk and pathogenesis. Mor...
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| Format: | Article |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Toxicology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/ftox.2023.1244596/full |
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| author | Bayan Alakhtar Cynthia Guilbert Nivetha Subramaniam Vincenza Caruana Vincenza Caruana Vincenza Caruana Kiran Makhani Carolyn J. Baglole Carolyn J. Baglole Carolyn J. Baglole Carolyn J. Baglole Koren K. Mann Koren K. Mann Koren K. Mann |
| author_facet | Bayan Alakhtar Cynthia Guilbert Nivetha Subramaniam Vincenza Caruana Vincenza Caruana Vincenza Caruana Kiran Makhani Carolyn J. Baglole Carolyn J. Baglole Carolyn J. Baglole Carolyn J. Baglole Koren K. Mann Koren K. Mann Koren K. Mann |
| author_sort | Bayan Alakhtar |
| collection | DOAJ |
| description | Introduction: Evidence suggests that e-cigarette use (vaping) increases cardiovascular disease risk, but decades are needed before people who vape would develop pathology. Thus, murine models of atherosclerosis can be utilized as tools to understand disease susceptibility, risk and pathogenesis. Moreover, there is a poor understanding of how risk factors for atherosclerosis (i.e., hyperlipidemia, high-fat diet) intersect with vaping to promote disease risk. Herein, we evaluated whether there was early evidence of atherosclerosis in an inducible hyperlipidemic mouse exposed to aerosol from commercial pod-style devices and e-liquid.Methods: Mice were injected with adeno-associated virus containing the human protein convertase subtilisin/kexin type 9 (PCSK9) variant to promote hyperlipidemia. These mice were fed a high-fat diet and exposed to room air or aerosol derived from JUUL pods containing polyethylene glycol/vegetable glycerin (PG/VG) or 5% nicotine with mango flavoring for 4 weeks; this timepoint was utilized to assess markers of atherosclerosis that may occur prior to the development of atherosclerotic plaques.Results: These data show that various parameters including weight, circulating lipoprotein/glucose levels, and splenic immune cells were significantly affected by exposure to PG/VG and/or nicotine-containing aerosols.Discussion: Not only can this mouse model be utilized for chronic vaping studies to assess the vascular pathology but these data support that vaping is not risk-free and may increase CVD outcomes later in life. |
| first_indexed | 2024-03-08T22:29:11Z |
| format | Article |
| id | doaj.art-342f357089b74edf812ca2357197e51b |
| institution | Directory Open Access Journal |
| issn | 2673-3080 |
| language | English |
| last_indexed | 2024-03-08T22:29:11Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Toxicology |
| spelling | doaj.art-342f357089b74edf812ca2357197e51b2023-12-18T06:58:08ZengFrontiers Media S.A.Frontiers in Toxicology2673-30802023-12-01510.3389/ftox.2023.12445961244596E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosisBayan Alakhtar0Cynthia Guilbert1Nivetha Subramaniam2Vincenza Caruana3Vincenza Caruana4Vincenza Caruana5Kiran Makhani6Carolyn J. Baglole7Carolyn J. Baglole8Carolyn J. Baglole9Carolyn J. Baglole10Koren K. Mann11Koren K. Mann12Koren K. Mann13Division of Experimental Medicine, Department of Medicine, McGill University, Montreal, QC, CanadaLady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC, CanadaDivision of Experimental Medicine, Department of Medicine, McGill University, Montreal, QC, CanadaLady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC, CanadaResearch Institute of the McGill University Health Centre, Montreal, QC, CanadaDepartment of Pharmacology and Therapeutics, McGill University, Montreal, QC, CanadaDivision of Experimental Medicine, Department of Medicine, McGill University, Montreal, QC, CanadaDivision of Experimental Medicine, Department of Medicine, McGill University, Montreal, QC, CanadaResearch Institute of the McGill University Health Centre, Montreal, QC, CanadaDepartment of Pharmacology and Therapeutics, McGill University, Montreal, QC, CanadaDepartment of Pathology, McGill University, Montreal, QC, CanadaDivision of Experimental Medicine, Department of Medicine, McGill University, Montreal, QC, CanadaLady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC, CanadaDepartment of Pharmacology and Therapeutics, McGill University, Montreal, QC, CanadaIntroduction: Evidence suggests that e-cigarette use (vaping) increases cardiovascular disease risk, but decades are needed before people who vape would develop pathology. Thus, murine models of atherosclerosis can be utilized as tools to understand disease susceptibility, risk and pathogenesis. Moreover, there is a poor understanding of how risk factors for atherosclerosis (i.e., hyperlipidemia, high-fat diet) intersect with vaping to promote disease risk. Herein, we evaluated whether there was early evidence of atherosclerosis in an inducible hyperlipidemic mouse exposed to aerosol from commercial pod-style devices and e-liquid.Methods: Mice were injected with adeno-associated virus containing the human protein convertase subtilisin/kexin type 9 (PCSK9) variant to promote hyperlipidemia. These mice were fed a high-fat diet and exposed to room air or aerosol derived from JUUL pods containing polyethylene glycol/vegetable glycerin (PG/VG) or 5% nicotine with mango flavoring for 4 weeks; this timepoint was utilized to assess markers of atherosclerosis that may occur prior to the development of atherosclerotic plaques.Results: These data show that various parameters including weight, circulating lipoprotein/glucose levels, and splenic immune cells were significantly affected by exposure to PG/VG and/or nicotine-containing aerosols.Discussion: Not only can this mouse model be utilized for chronic vaping studies to assess the vascular pathology but these data support that vaping is not risk-free and may increase CVD outcomes later in life.https://www.frontiersin.org/articles/10.3389/ftox.2023.1244596/fulle-cigaretteatherosclerosisvapingmouse modelhyperlipidaemia |
| spellingShingle | Bayan Alakhtar Cynthia Guilbert Nivetha Subramaniam Vincenza Caruana Vincenza Caruana Vincenza Caruana Kiran Makhani Carolyn J. Baglole Carolyn J. Baglole Carolyn J. Baglole Carolyn J. Baglole Koren K. Mann Koren K. Mann Koren K. Mann E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis Frontiers in Toxicology e-cigarette atherosclerosis vaping mouse model hyperlipidaemia |
| title | E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis |
| title_full | E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis |
| title_fullStr | E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis |
| title_full_unstemmed | E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis |
| title_short | E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis |
| title_sort | e cigarette exposure causes early pro atherogenic changes in an inducible murine model of atherosclerosis |
| topic | e-cigarette atherosclerosis vaping mouse model hyperlipidaemia |
| url | https://www.frontiersin.org/articles/10.3389/ftox.2023.1244596/full |
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