Effects of Luteolin-7-O-Glucoside on Intestinal Microbiota Dysbiosis and Drug Resistance Transmission Caused by <i>Raoultella ornithinolytica</i> B1645-1: Modulating the Composition of Intestinal Microbiota and Promoting the Transfer of <i>bla</i><sub>NDM-1</sub> Gene from Genus <i>Enterococcus</i> to <i>Lactobacillus</i> in Mice
<i>Raoultella ornithinolytica</i> is an <i>Enterobacteriaceae</i> bacterium that can infect both humans and animals, while luteolin-7-O-glucoside (IOG) is a flavonoid that has broad effects on the intestinal microbiota of healthy animals. However, current studies lack suffici...
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MDPI AG
2023-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/10/2477 |
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| author | Zhaomeng Wu Ronghui Gou Longhua Sha Chunfang Yu Lixue Meng Zhixiong Jin |
| author_facet | Zhaomeng Wu Ronghui Gou Longhua Sha Chunfang Yu Lixue Meng Zhixiong Jin |
| author_sort | Zhaomeng Wu |
| collection | DOAJ |
| description | <i>Raoultella ornithinolytica</i> is an <i>Enterobacteriaceae</i> bacterium that can infect both humans and animals, while luteolin-7-O-glucoside (IOG) is a flavonoid that has broad effects on the intestinal microbiota of healthy animals. However, current studies lack sufficient data on intestinal microbiota dysbiosis and drug resistance transmission caused by <i>R. ornithinolytica</i> and the possible role of IOG. In this study, BALB/c mice were infected with <i>R. ornithinolytica</i> carrying <i>bla</i><sub>NDM-1</sub> gene and treated with IOG (3 mg/kg·d and 6 mg/kg·d) to analyze the diversity of intestinal microbiota and the transfer of <i>bla</i><sub>NDM-1</sub> between bacteria. The findings indicated that <i>R. ornithinolytica</i> B1645-1 exhibited a significant ability to enhance the <i>Firmicutes</i>/<i>Bacteroidota</i> ratio and increase the relative abundance of <i>Lactobacillus</i> and <i>Bacillus</i> after 48 h, where as 6 mg/kg·d IOG had an opposite effect. Moreover, <i>R. ornithinolytica</i> B1645-1 facilitated the emergence of drug-resistant bacteria and promoted <i>bla</i><sub>NDM-1</sub> gene transfer in <i>Enterococcus</i>, <i>Escherichia</i>, <i>Klebsiella</i>, <i>Acinetobacter</i>, <i>Bacillus</i>, <i>Brevibacterium</i>, and <i>Lactobacillus</i>. <i>Enterococcus</i> was the predominant genus at 48 h. Surprisingly, 6 mg/kg·d IOG significantly inhibited the production of drug-resistant bacteria and promoted <i>bla</i><sub>NDM-1</sub> gene transfer from <i>Enterococcus</i> to <i>Lactobacillus</i> at 144 h. However, the role of <i>Lactobacillus</i> as a recipient for drug-resistant genes should be of more concern. |
| first_indexed | 2024-03-10T21:01:24Z |
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| id | doaj.art-342fa8a42b5547a68f0b882cc93e694a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T21:01:24Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-342fa8a42b5547a68f0b882cc93e694a2023-11-19T17:27:21ZengMDPI AGMicroorganisms2076-26072023-10-011110247710.3390/microorganisms11102477Effects of Luteolin-7-O-Glucoside on Intestinal Microbiota Dysbiosis and Drug Resistance Transmission Caused by <i>Raoultella ornithinolytica</i> B1645-1: Modulating the Composition of Intestinal Microbiota and Promoting the Transfer of <i>bla</i><sub>NDM-1</sub> Gene from Genus <i>Enterococcus</i> to <i>Lactobacillus</i> in MiceZhaomeng Wu0Ronghui Gou1Longhua Sha2Chunfang Yu3Lixue Meng4Zhixiong Jin5Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, ChinaHubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, ChinaHubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, ChinaHubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, ChinaHubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, ChinaHubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, China<i>Raoultella ornithinolytica</i> is an <i>Enterobacteriaceae</i> bacterium that can infect both humans and animals, while luteolin-7-O-glucoside (IOG) is a flavonoid that has broad effects on the intestinal microbiota of healthy animals. However, current studies lack sufficient data on intestinal microbiota dysbiosis and drug resistance transmission caused by <i>R. ornithinolytica</i> and the possible role of IOG. In this study, BALB/c mice were infected with <i>R. ornithinolytica</i> carrying <i>bla</i><sub>NDM-1</sub> gene and treated with IOG (3 mg/kg·d and 6 mg/kg·d) to analyze the diversity of intestinal microbiota and the transfer of <i>bla</i><sub>NDM-1</sub> between bacteria. The findings indicated that <i>R. ornithinolytica</i> B1645-1 exhibited a significant ability to enhance the <i>Firmicutes</i>/<i>Bacteroidota</i> ratio and increase the relative abundance of <i>Lactobacillus</i> and <i>Bacillus</i> after 48 h, where as 6 mg/kg·d IOG had an opposite effect. Moreover, <i>R. ornithinolytica</i> B1645-1 facilitated the emergence of drug-resistant bacteria and promoted <i>bla</i><sub>NDM-1</sub> gene transfer in <i>Enterococcus</i>, <i>Escherichia</i>, <i>Klebsiella</i>, <i>Acinetobacter</i>, <i>Bacillus</i>, <i>Brevibacterium</i>, and <i>Lactobacillus</i>. <i>Enterococcus</i> was the predominant genus at 48 h. Surprisingly, 6 mg/kg·d IOG significantly inhibited the production of drug-resistant bacteria and promoted <i>bla</i><sub>NDM-1</sub> gene transfer from <i>Enterococcus</i> to <i>Lactobacillus</i> at 144 h. However, the role of <i>Lactobacillus</i> as a recipient for drug-resistant genes should be of more concern.https://www.mdpi.com/2076-2607/11/10/2477luteolin-7-O-glucosideintestinal microbiota dysbiosis<i>Raoultella ornithinolytica</i>drug resistance transmission<i>bla</i><sub>NDM-1</sub> |
| spellingShingle | Zhaomeng Wu Ronghui Gou Longhua Sha Chunfang Yu Lixue Meng Zhixiong Jin Effects of Luteolin-7-O-Glucoside on Intestinal Microbiota Dysbiosis and Drug Resistance Transmission Caused by <i>Raoultella ornithinolytica</i> B1645-1: Modulating the Composition of Intestinal Microbiota and Promoting the Transfer of <i>bla</i><sub>NDM-1</sub> Gene from Genus <i>Enterococcus</i> to <i>Lactobacillus</i> in Mice Microorganisms luteolin-7-O-glucoside intestinal microbiota dysbiosis <i>Raoultella ornithinolytica</i> drug resistance transmission <i>bla</i><sub>NDM-1</sub> |
| title | Effects of Luteolin-7-O-Glucoside on Intestinal Microbiota Dysbiosis and Drug Resistance Transmission Caused by <i>Raoultella ornithinolytica</i> B1645-1: Modulating the Composition of Intestinal Microbiota and Promoting the Transfer of <i>bla</i><sub>NDM-1</sub> Gene from Genus <i>Enterococcus</i> to <i>Lactobacillus</i> in Mice |
| title_full | Effects of Luteolin-7-O-Glucoside on Intestinal Microbiota Dysbiosis and Drug Resistance Transmission Caused by <i>Raoultella ornithinolytica</i> B1645-1: Modulating the Composition of Intestinal Microbiota and Promoting the Transfer of <i>bla</i><sub>NDM-1</sub> Gene from Genus <i>Enterococcus</i> to <i>Lactobacillus</i> in Mice |
| title_fullStr | Effects of Luteolin-7-O-Glucoside on Intestinal Microbiota Dysbiosis and Drug Resistance Transmission Caused by <i>Raoultella ornithinolytica</i> B1645-1: Modulating the Composition of Intestinal Microbiota and Promoting the Transfer of <i>bla</i><sub>NDM-1</sub> Gene from Genus <i>Enterococcus</i> to <i>Lactobacillus</i> in Mice |
| title_full_unstemmed | Effects of Luteolin-7-O-Glucoside on Intestinal Microbiota Dysbiosis and Drug Resistance Transmission Caused by <i>Raoultella ornithinolytica</i> B1645-1: Modulating the Composition of Intestinal Microbiota and Promoting the Transfer of <i>bla</i><sub>NDM-1</sub> Gene from Genus <i>Enterococcus</i> to <i>Lactobacillus</i> in Mice |
| title_short | Effects of Luteolin-7-O-Glucoside on Intestinal Microbiota Dysbiosis and Drug Resistance Transmission Caused by <i>Raoultella ornithinolytica</i> B1645-1: Modulating the Composition of Intestinal Microbiota and Promoting the Transfer of <i>bla</i><sub>NDM-1</sub> Gene from Genus <i>Enterococcus</i> to <i>Lactobacillus</i> in Mice |
| title_sort | effects of luteolin 7 o glucoside on intestinal microbiota dysbiosis and drug resistance transmission caused by i raoultella ornithinolytica i b1645 1 modulating the composition of intestinal microbiota and promoting the transfer of i bla i sub ndm 1 sub gene from genus i enterococcus i to i lactobacillus i in mice |
| topic | luteolin-7-O-glucoside intestinal microbiota dysbiosis <i>Raoultella ornithinolytica</i> drug resistance transmission <i>bla</i><sub>NDM-1</sub> |
| url | https://www.mdpi.com/2076-2607/11/10/2477 |
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