Study of IL-12 Agonists in Macrophages from Patients with Healing and Non-Healing Forms of Cutaneous leishmaniasis
Background: Cutaneous leishmaniasis (CL) is a self-healing disease; however, due to some reasons, in a few cases the lesion develops to a non-healing form of disease. The initial encounter of Leishmania with its host’s innate immune system is important in the outcome of infection. Although, tremendo...
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| Format: | Article |
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Isfahan University of Medical Sciences
2013-11-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2523 |
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| author | Sepideh Tolouei Seyed Hossein Hejazi Seyed Javad Hasheminia Reza Arjmand Ali Khamesipour Mohamad Ali Nilforoushzadeh |
| author_facet | Sepideh Tolouei Seyed Hossein Hejazi Seyed Javad Hasheminia Reza Arjmand Ali Khamesipour Mohamad Ali Nilforoushzadeh |
| author_sort | Sepideh Tolouei |
| collection | DOAJ |
| description | Background: Cutaneous leishmaniasis (CL) is a self-healing disease; however, due to some reasons, in a few cases the lesion develops to a non-healing form of disease. The initial encounter of Leishmania with its host’s innate immune system is important in the outcome of infection. Although, tremendous data is available in murine model of leishmaniasis but immunological surrogate marker(s) of healing and protection in human is not yet well-defined. In this study, the level of IL-23 and IL-27 produced by peripheral blood derived macrophages from patients with healing or non-healing form of cutaneous leishmaniasis lesion were determined to explore whether IL-23 or IL-27 plays a protection role in healing process of cutaneous lesions induced by Leishmania major (L. major).
Methods: 26 six patients resident in Isfahan Province, Iran, with healing or non-healing forms of cutaneous leishmaniasis caused by L. major were selected. In-vitro productions of IL-23 and IL-27 by peripheral blood derived macrophages, before and after stimulation with live L. major (MRHO/IR/75/ER) promastigotes were evaluated in these two groups and control group using the enzyme-linked immunosorbent assay (ELISA) method.
Findings: The mean difference production of IL-23 and IL-27 from macrophages of patients with healing form of lesion was significantly higher than patients with non-healing form. The levels of IL-23 and IL-27 in culture supernatants before and after stimulation in healing form of disease was significantly higher than non-healing form (P < 0.001).
Conclusion: It seems that IL-12 agonists might play a protection role in human leishmaniasis and further studies are needed to understand the role of these cytokine in cutaneous leishmaniasis. |
| first_indexed | 2024-03-12T07:01:59Z |
| format | Article |
| id | doaj.art-34330da660634b2690b6b4bf0f0fec00 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T07:01:59Z |
| publishDate | 2013-11-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-34330da660634b2690b6b4bf0f0fec002023-09-02T23:39:24ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2013-11-0131252143514421417Study of IL-12 Agonists in Macrophages from Patients with Healing and Non-Healing Forms of Cutaneous leishmaniasisSepideh Tolouei0Seyed Hossein Hejazi1Seyed Javad Hasheminia2Reza Arjmand3Ali Khamesipour4Mohamad Ali Nilforoushzadeh5Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Skin Disease and Leishmaniasis Research Center AND Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranPhD Student, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranPhD Student, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranProfessor, Center for Research and Training in Skin Disease and Leprosy, Tehran University of Medical Sciences, Tehran, IranAssociate Professor, Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, IranBackground: Cutaneous leishmaniasis (CL) is a self-healing disease; however, due to some reasons, in a few cases the lesion develops to a non-healing form of disease. The initial encounter of Leishmania with its host’s innate immune system is important in the outcome of infection. Although, tremendous data is available in murine model of leishmaniasis but immunological surrogate marker(s) of healing and protection in human is not yet well-defined. In this study, the level of IL-23 and IL-27 produced by peripheral blood derived macrophages from patients with healing or non-healing form of cutaneous leishmaniasis lesion were determined to explore whether IL-23 or IL-27 plays a protection role in healing process of cutaneous lesions induced by Leishmania major (L. major). Methods: 26 six patients resident in Isfahan Province, Iran, with healing or non-healing forms of cutaneous leishmaniasis caused by L. major were selected. In-vitro productions of IL-23 and IL-27 by peripheral blood derived macrophages, before and after stimulation with live L. major (MRHO/IR/75/ER) promastigotes were evaluated in these two groups and control group using the enzyme-linked immunosorbent assay (ELISA) method. Findings: The mean difference production of IL-23 and IL-27 from macrophages of patients with healing form of lesion was significantly higher than patients with non-healing form. The levels of IL-23 and IL-27 in culture supernatants before and after stimulation in healing form of disease was significantly higher than non-healing form (P < 0.001). Conclusion: It seems that IL-12 agonists might play a protection role in human leishmaniasis and further studies are needed to understand the role of these cytokine in cutaneous leishmaniasis.http://jims.mui.ac.ir/index.php/jims/article/view/2523AgonistIL-23IL-27MacrophagesPromastigoteCutaneous leishmaniasis |
| spellingShingle | Sepideh Tolouei Seyed Hossein Hejazi Seyed Javad Hasheminia Reza Arjmand Ali Khamesipour Mohamad Ali Nilforoushzadeh Study of IL-12 Agonists in Macrophages from Patients with Healing and Non-Healing Forms of Cutaneous leishmaniasis مجله دانشکده پزشکی اصفهان Agonist IL-23 IL-27 Macrophages Promastigote Cutaneous leishmaniasis |
| title | Study of IL-12 Agonists in Macrophages from Patients with Healing and Non-Healing Forms of Cutaneous leishmaniasis |
| title_full | Study of IL-12 Agonists in Macrophages from Patients with Healing and Non-Healing Forms of Cutaneous leishmaniasis |
| title_fullStr | Study of IL-12 Agonists in Macrophages from Patients with Healing and Non-Healing Forms of Cutaneous leishmaniasis |
| title_full_unstemmed | Study of IL-12 Agonists in Macrophages from Patients with Healing and Non-Healing Forms of Cutaneous leishmaniasis |
| title_short | Study of IL-12 Agonists in Macrophages from Patients with Healing and Non-Healing Forms of Cutaneous leishmaniasis |
| title_sort | study of il 12 agonists in macrophages from patients with healing and non healing forms of cutaneous leishmaniasis |
| topic | Agonist IL-23 IL-27 Macrophages Promastigote Cutaneous leishmaniasis |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/2523 |
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