Effect of Varying Proportions of Lignin and Cellulose Supplements on Immune Function and Lymphoid Organs of Layer Poultry (Gallus gallus)
To determine the benefits of different types or proportions of insoluble fiber components on growth and immunity, 4-week-old commercial layer pullets were fed supplements containing different proportions of purified lignin and cellulose or a commercial lignocellulose supplement. The 64 Hy-Line Brown...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2019-01-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/56/1/56_0180032/_html/-char/en |
| _version_ | 1797817000548892672 |
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| author | Sherzad M. Hussein Theresa L. Frankel |
| author_facet | Sherzad M. Hussein Theresa L. Frankel |
| author_sort | Sherzad M. Hussein |
| collection | DOAJ |
| description | To determine the benefits of different types or proportions of insoluble fiber components on growth and immunity, 4-week-old commercial layer pullets were fed supplements containing different proportions of purified lignin and cellulose or a commercial lignocellulose supplement. The 64 Hy-Line Brown pullets were provided basal diets supplemented with 1 g fiber per 100 g diet. The supplements included a commercial lignocellulose, Arbocel® RC fine (group A) with cellulose to lignin ratio of approximately 3:1, cellulose (group Ce), a 3:1 mixture of cellulose: lignin (group Ce3Lig1), and a 2:1 mixture of cellulose: lignin (group Ce2Lig1). After 3 weeks, innate immune function was measured in terms of heterophil phagocytosis and oxidative burst (n=8). After 4 weeks, ex vivo stimulated lymphocyte proliferation was determined for assessment of cell-mediated immune function (n=7). All pullets were killed at 9 weeks of age and lymphoid organs were weighed (n=16) and small intestinal Peyer's patches (PP) were measured (n=8). Pullets in both A and Ce3Lig1 groups had heavier (P<0.05) body and bursa of Fabricius weights. The number of PP in group A was higher (P<0.05) than in group Ce. The percentage of heterophil phagocytosis in A and Ce3Lig1 groups were higher (P<0.05) than in group Ce, and oxidative burst of group A was higher (P<0.05) than that of group Ce. Addition of 1% Arbocel or 1% Ce3Lig1 to the diet of layer pullets from 4 to 9 weeks of age significantly improved their growth and innate immune function compared to group Ce. This suggests that lignin either modulates the effect of cellulose or has specific mechanisms of action in the gut that improves growth and immunity. The proportion of lignin to cellulose may also be important for growth and immune function. |
| first_indexed | 2024-03-13T08:46:49Z |
| format | Article |
| id | doaj.art-343354c92f904d5fbb45e64e1ba185cc |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:46:49Z |
| publishDate | 2019-01-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-343354c92f904d5fbb45e64e1ba185cc2023-05-30T01:41:50ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862019-01-01561717710.2141/jpsa.0180032jpsaEffect of Varying Proportions of Lignin and Cellulose Supplements on Immune Function and Lymphoid Organs of Layer Poultry (Gallus gallus)Sherzad M. Hussein0Theresa L. Frankel1Department of Physiology, Anatomy and Microbiology, School of Life Sciences, La Trobe UniversityDepartment of Physiology, Anatomy and Microbiology, School of Life Sciences, La Trobe UniversityTo determine the benefits of different types or proportions of insoluble fiber components on growth and immunity, 4-week-old commercial layer pullets were fed supplements containing different proportions of purified lignin and cellulose or a commercial lignocellulose supplement. The 64 Hy-Line Brown pullets were provided basal diets supplemented with 1 g fiber per 100 g diet. The supplements included a commercial lignocellulose, Arbocel® RC fine (group A) with cellulose to lignin ratio of approximately 3:1, cellulose (group Ce), a 3:1 mixture of cellulose: lignin (group Ce3Lig1), and a 2:1 mixture of cellulose: lignin (group Ce2Lig1). After 3 weeks, innate immune function was measured in terms of heterophil phagocytosis and oxidative burst (n=8). After 4 weeks, ex vivo stimulated lymphocyte proliferation was determined for assessment of cell-mediated immune function (n=7). All pullets were killed at 9 weeks of age and lymphoid organs were weighed (n=16) and small intestinal Peyer's patches (PP) were measured (n=8). Pullets in both A and Ce3Lig1 groups had heavier (P<0.05) body and bursa of Fabricius weights. The number of PP in group A was higher (P<0.05) than in group Ce. The percentage of heterophil phagocytosis in A and Ce3Lig1 groups were higher (P<0.05) than in group Ce, and oxidative burst of group A was higher (P<0.05) than that of group Ce. Addition of 1% Arbocel or 1% Ce3Lig1 to the diet of layer pullets from 4 to 9 weeks of age significantly improved their growth and innate immune function compared to group Ce. This suggests that lignin either modulates the effect of cellulose or has specific mechanisms of action in the gut that improves growth and immunity. The proportion of lignin to cellulose may also be important for growth and immune function.https://www.jstage.jst.go.jp/article/jpsa/56/1/56_0180032/_html/-char/enheterophilsinsoluble fiberlymphocytesoxidative burstpeyer's patchesphagocytosis |
| spellingShingle | Sherzad M. Hussein Theresa L. Frankel Effect of Varying Proportions of Lignin and Cellulose Supplements on Immune Function and Lymphoid Organs of Layer Poultry (Gallus gallus) The Journal of Poultry Science heterophils insoluble fiber lymphocytes oxidative burst peyer's patches phagocytosis |
| title | Effect of Varying Proportions of Lignin and Cellulose Supplements on Immune Function and Lymphoid Organs of Layer Poultry (Gallus gallus) |
| title_full | Effect of Varying Proportions of Lignin and Cellulose Supplements on Immune Function and Lymphoid Organs of Layer Poultry (Gallus gallus) |
| title_fullStr | Effect of Varying Proportions of Lignin and Cellulose Supplements on Immune Function and Lymphoid Organs of Layer Poultry (Gallus gallus) |
| title_full_unstemmed | Effect of Varying Proportions of Lignin and Cellulose Supplements on Immune Function and Lymphoid Organs of Layer Poultry (Gallus gallus) |
| title_short | Effect of Varying Proportions of Lignin and Cellulose Supplements on Immune Function and Lymphoid Organs of Layer Poultry (Gallus gallus) |
| title_sort | effect of varying proportions of lignin and cellulose supplements on immune function and lymphoid organs of layer poultry gallus gallus |
| topic | heterophils insoluble fiber lymphocytes oxidative burst peyer's patches phagocytosis |
| url | https://www.jstage.jst.go.jp/article/jpsa/56/1/56_0180032/_html/-char/en |
| work_keys_str_mv | AT sherzadmhussein effectofvaryingproportionsofligninandcellulosesupplementsonimmunefunctionandlymphoidorgansoflayerpoultrygallusgallus AT theresalfrankel effectofvaryingproportionsofligninandcellulosesupplementsonimmunefunctionandlymphoidorgansoflayerpoultrygallusgallus |