Evolutionary intraembryonic origin of vertebrate hematopoietic stem cells in the elasmobranch spleen
The electric ray (Torpedo Marmorata Risso) provides an animal model for the detection of early intraembryonic hemopoietic stem cells in sea vertebrates. The spleen of this bone-marrowless vertebrate appears to be the major site of hemopoietic stem cell differentiation during development and in adult...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2018-12-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | https://ejh.it/index.php/ejh/article/view/2987 |
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| author | Rosa Manca Chester A. Glomski Alessandra Pica |
| author_facet | Rosa Manca Chester A. Glomski Alessandra Pica |
| author_sort | Rosa Manca |
| collection | DOAJ |
| description | The electric ray (Torpedo Marmorata Risso) provides an animal model for the detection of early intraembryonic hemopoietic stem cells in sea vertebrates. The spleen of this bone-marrowless vertebrate appears to be the major site of hemopoietic stem cell differentiation during development and in adulthood. Splenic development in this species was investigated and hemopoietic stem cells were detected in this organ by immunocytochemistry utilizing CD34 and CD38 antibodies. At stage I (2-cm-long embryos with external gills), the spleen contains only mesenchymal cells. At stage II (3-4 cm-long embryos with a discoidal shape and internal gills), an initial red pulp was observed in the spleen, without immunostained cells. At stage III (10-11-cm-long embryos), the spleen contained well-developed white pulp, red pulp and ellipsoids. Image analysis at stage III showed four cell populations, i.e. CD34+/CD38-, CD34+/CD38+, CD34-/CD38+, and CD34-/CD38- cells. The present findings, obtained from an elasmobranch, indicate that the CD34 and CD38 phenotypes are conserved through vertebrate evolution. |
| first_indexed | 2024-12-22T06:36:17Z |
| format | Article |
| id | doaj.art-34356ed6206e4613b547d3c25ff36278 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-22T06:36:17Z |
| publishDate | 2018-12-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-34356ed6206e4613b547d3c25ff362782022-12-21T18:35:34ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062018-12-0162410.4081/ejh.2018.2987Evolutionary intraembryonic origin of vertebrate hematopoietic stem cells in the elasmobranch spleenRosa Manca0Chester A. Glomski1Alessandra Pica2University of Naples Federico II, Department of BiologyJacobs School of Medicine, State University of New York, Department of Pathology and Anatomical SciencesUniversity of Naples Federico II, Department of BiologyThe electric ray (Torpedo Marmorata Risso) provides an animal model for the detection of early intraembryonic hemopoietic stem cells in sea vertebrates. The spleen of this bone-marrowless vertebrate appears to be the major site of hemopoietic stem cell differentiation during development and in adulthood. Splenic development in this species was investigated and hemopoietic stem cells were detected in this organ by immunocytochemistry utilizing CD34 and CD38 antibodies. At stage I (2-cm-long embryos with external gills), the spleen contains only mesenchymal cells. At stage II (3-4 cm-long embryos with a discoidal shape and internal gills), an initial red pulp was observed in the spleen, without immunostained cells. At stage III (10-11-cm-long embryos), the spleen contained well-developed white pulp, red pulp and ellipsoids. Image analysis at stage III showed four cell populations, i.e. CD34+/CD38-, CD34+/CD38+, CD34-/CD38+, and CD34-/CD38- cells. The present findings, obtained from an elasmobranch, indicate that the CD34 and CD38 phenotypes are conserved through vertebrate evolution.https://ejh.it/index.php/ejh/article/view/2987Embryonic hemopoiesisCD34 /CD38- cellsprogenitor cellselasmobranchsTorpedoesHSC |
| spellingShingle | Rosa Manca Chester A. Glomski Alessandra Pica Evolutionary intraembryonic origin of vertebrate hematopoietic stem cells in the elasmobranch spleen European Journal of Histochemistry Embryonic hemopoiesis CD34 /CD38- cells progenitor cells elasmobranchs Torpedoes HSC |
| title | Evolutionary intraembryonic origin of vertebrate hematopoietic stem cells in the elasmobranch spleen |
| title_full | Evolutionary intraembryonic origin of vertebrate hematopoietic stem cells in the elasmobranch spleen |
| title_fullStr | Evolutionary intraembryonic origin of vertebrate hematopoietic stem cells in the elasmobranch spleen |
| title_full_unstemmed | Evolutionary intraembryonic origin of vertebrate hematopoietic stem cells in the elasmobranch spleen |
| title_short | Evolutionary intraembryonic origin of vertebrate hematopoietic stem cells in the elasmobranch spleen |
| title_sort | evolutionary intraembryonic origin of vertebrate hematopoietic stem cells in the elasmobranch spleen |
| topic | Embryonic hemopoiesis CD34 /CD38- cells progenitor cells elasmobranchs Torpedoes HSC |
| url | https://ejh.it/index.php/ejh/article/view/2987 |
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