Study on the structure characterization and moisturizing effect of Tremella polysaccharide fermented from GCMCC5.39
The structure and moisture retention of Tremella polysaccharide fermented from GCMCC5.39 (FTP) were evaluated. After UV, infrared spectrum analysis, HPAEC-PAD, HPSEC and 1 D NMR analysis, the composition of the purified FTP was determined. Purified components of fermented Tremella polysaccharide (FT...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2021-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021000562 |
| _version_ | 1797706910554652672 |
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| author | Meng Yang Zilong Zhang Yan He Chengliang Li Jinmei Wang Xia Ma |
| author_facet | Meng Yang Zilong Zhang Yan He Chengliang Li Jinmei Wang Xia Ma |
| author_sort | Meng Yang |
| collection | DOAJ |
| description | The structure and moisture retention of Tremella polysaccharide fermented from GCMCC5.39 (FTP) were evaluated. After UV, infrared spectrum analysis, HPAEC-PAD, HPSEC and 1 D NMR analysis, the composition of the purified FTP was determined. Purified components of fermented Tremella polysaccharide (FTPS) was made of galactose, mannose, glucose, galactosmine, glucosamine, and contain a large amount of hydroxyl, carbonyl and amino groups. FTPS was α -neutral pyranose without uronic acid. FTPS-1 and FTPS-2 were obtained after purification by DEAE-Sepharose Fast Flow Column. The molecular weights of FTPS-1 and FTPS-2 were 25 722 and 177 263 Da. FTPS-2 had a better ability to prevent moisture loss, and the optimal moisture retention period was 0–4 h. FTPS-2 could significantly increase the moisture content of the skin epidermis and showed a dose-concentration relationship. The effect of FTPS-2 on the expression of different moisturizing genes was evaluated in a human skin keratinocyte model. The results showed that FTPS-2 has no cytotoxicity, and could significantly promote AQP3, TGM1, CASP14, HYAL2, FLG gene expression level in HaCaT cells. It has the most significant influence at HYAL2 protein expression on 50 μg/mL. |
| first_indexed | 2024-03-12T05:59:23Z |
| format | Article |
| id | doaj.art-34374e2379c247f9942d6996a63fcf52 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T05:59:23Z |
| publishDate | 2021-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-34374e2379c247f9942d6996a63fcf522023-09-03T04:19:19ZengTsinghua University PressFood Science and Human Wellness2213-45302021-07-01104471479Study on the structure characterization and moisturizing effect of Tremella polysaccharide fermented from GCMCC5.39Meng Yang0Zilong Zhang1Yan He2Chengliang Li3Jinmei Wang4Xia Ma5School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, ChinaShanghai International Travel Healthcare Center, Shanghai Customs District P.R. China, Shanghai 200335, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, ChinaLaibo Pharmaceutical Technology (Shanghai) Co. Ltd, Shanghai 201418, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Corresponding author.School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China; Corresponding author.The structure and moisture retention of Tremella polysaccharide fermented from GCMCC5.39 (FTP) were evaluated. After UV, infrared spectrum analysis, HPAEC-PAD, HPSEC and 1 D NMR analysis, the composition of the purified FTP was determined. Purified components of fermented Tremella polysaccharide (FTPS) was made of galactose, mannose, glucose, galactosmine, glucosamine, and contain a large amount of hydroxyl, carbonyl and amino groups. FTPS was α -neutral pyranose without uronic acid. FTPS-1 and FTPS-2 were obtained after purification by DEAE-Sepharose Fast Flow Column. The molecular weights of FTPS-1 and FTPS-2 were 25 722 and 177 263 Da. FTPS-2 had a better ability to prevent moisture loss, and the optimal moisture retention period was 0–4 h. FTPS-2 could significantly increase the moisture content of the skin epidermis and showed a dose-concentration relationship. The effect of FTPS-2 on the expression of different moisturizing genes was evaluated in a human skin keratinocyte model. The results showed that FTPS-2 has no cytotoxicity, and could significantly promote AQP3, TGM1, CASP14, HYAL2, FLG gene expression level in HaCaT cells. It has the most significant influence at HYAL2 protein expression on 50 μg/mL.http://www.sciencedirect.com/science/article/pii/S2213453021000562PolysaccharideTremella spp.Structure identificationMoisturizing. |
| spellingShingle | Meng Yang Zilong Zhang Yan He Chengliang Li Jinmei Wang Xia Ma Study on the structure characterization and moisturizing effect of Tremella polysaccharide fermented from GCMCC5.39 Food Science and Human Wellness Polysaccharide Tremella spp. Structure identification Moisturizing. |
| title | Study on the structure characterization and moisturizing effect of Tremella polysaccharide fermented from GCMCC5.39 |
| title_full | Study on the structure characterization and moisturizing effect of Tremella polysaccharide fermented from GCMCC5.39 |
| title_fullStr | Study on the structure characterization and moisturizing effect of Tremella polysaccharide fermented from GCMCC5.39 |
| title_full_unstemmed | Study on the structure characterization and moisturizing effect of Tremella polysaccharide fermented from GCMCC5.39 |
| title_short | Study on the structure characterization and moisturizing effect of Tremella polysaccharide fermented from GCMCC5.39 |
| title_sort | study on the structure characterization and moisturizing effect of tremella polysaccharide fermented from gcmcc5 39 |
| topic | Polysaccharide Tremella spp. Structure identification Moisturizing. |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021000562 |
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