Synchrotron-based characterization of arthroprosthetic CoCrMo particles in human bone marrow
Abstract Particles released from cobalt-chromium-molybdenum (CoCrMo) alloys are considered common elicitors of chronic inflammatory adverse effects. There is a lack of data demonstrating particle numbers, size distribution and elemental composition of bone marrow resident particles which would allow...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2022-06-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-022-06675-2 |
| _version_ | 1811242261206269952 |
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| author | Janosch Schoon Bernhard Hesse Remi Tucoulou Sven Geissler Melanie Ort Georg N. Duda Carsten Perka Georgi I. Wassilew Giorgio Perino Anastasia Rakow |
| author_facet | Janosch Schoon Bernhard Hesse Remi Tucoulou Sven Geissler Melanie Ort Georg N. Duda Carsten Perka Georgi I. Wassilew Giorgio Perino Anastasia Rakow |
| author_sort | Janosch Schoon |
| collection | DOAJ |
| description | Abstract Particles released from cobalt-chromium-molybdenum (CoCrMo) alloys are considered common elicitors of chronic inflammatory adverse effects. There is a lack of data demonstrating particle numbers, size distribution and elemental composition of bone marrow resident particles which would allow for implementation of clinically relevant test strategies in bone marrow models at different degrees of exposure. The aim of this study was to investigate metal particle exposure in human periprosthetic bone marrow of three types of arthroplasty implants. Periprosthetic bone marrow sections from eight patients exposed to CoCrMo particles were analyzed via spatially resolved and synchrotron-based nanoscopic X-ray fluorescence imaging. These analyses revealed lognormal particle size distribution patterns predominantly towards the nanoscale. Analyses of particle numbers and normalization to bone marrow volume and bone marrow cell number indicated particle concentrations of up to 1 × 1011 particles/ml bone marrow or 2 × 104 particles/bone marrow cell, respectively. Analyses of elemental ratios of CoCrMo particles showed that particularly the particles’ Co content depends on particle size. The obtained data point towards Co release from arthroprosthetic particles in the course of dealloying and degradation processes of larger particles within periprosthetic bone marrow. This is the first study providing data based on metal particle analyses to be used for future in vitro and in vivo studies of possible toxic effects in human bone marrow following exposure to arthroprosthetic CoCrMo particles of different concentration, size, and elemental composition. Graphical abstract |
| first_indexed | 2024-04-12T13:49:08Z |
| format | Article |
| id | doaj.art-3438335bb97845eca438e8c2c80e4941 |
| institution | Directory Open Access Journal |
| issn | 1573-4838 |
| language | English |
| last_indexed | 2024-04-12T13:49:08Z |
| publishDate | 2022-06-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj.art-3438335bb97845eca438e8c2c80e49412022-12-22T03:30:35ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382022-06-0133611410.1007/s10856-022-06675-2Synchrotron-based characterization of arthroprosthetic CoCrMo particles in human bone marrowJanosch Schoon0Bernhard Hesse1Remi Tucoulou2Sven Geissler3Melanie Ort4Georg N. Duda5Carsten Perka6Georgi I. Wassilew7Giorgio Perino8Anastasia Rakow9Center for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine GreifswaldXploraytion GmbHESRF-The European SynchrotronBIH Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité - Universitätsmedizin BerlinJulius Wolff Institute, Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Augustenburger Platz 1Julius Wolff Institute, Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Augustenburger Platz 1Center for Musculoskeletal Surgery, Charité - Universitätsmedizin BerlinCenter for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine GreifswaldCenter for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine GreifswaldCenter for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine GreifswaldAbstract Particles released from cobalt-chromium-molybdenum (CoCrMo) alloys are considered common elicitors of chronic inflammatory adverse effects. There is a lack of data demonstrating particle numbers, size distribution and elemental composition of bone marrow resident particles which would allow for implementation of clinically relevant test strategies in bone marrow models at different degrees of exposure. The aim of this study was to investigate metal particle exposure in human periprosthetic bone marrow of three types of arthroplasty implants. Periprosthetic bone marrow sections from eight patients exposed to CoCrMo particles were analyzed via spatially resolved and synchrotron-based nanoscopic X-ray fluorescence imaging. These analyses revealed lognormal particle size distribution patterns predominantly towards the nanoscale. Analyses of particle numbers and normalization to bone marrow volume and bone marrow cell number indicated particle concentrations of up to 1 × 1011 particles/ml bone marrow or 2 × 104 particles/bone marrow cell, respectively. Analyses of elemental ratios of CoCrMo particles showed that particularly the particles’ Co content depends on particle size. The obtained data point towards Co release from arthroprosthetic particles in the course of dealloying and degradation processes of larger particles within periprosthetic bone marrow. This is the first study providing data based on metal particle analyses to be used for future in vitro and in vivo studies of possible toxic effects in human bone marrow following exposure to arthroprosthetic CoCrMo particles of different concentration, size, and elemental composition. Graphical abstracthttps://doi.org/10.1007/s10856-022-06675-2 |
| spellingShingle | Janosch Schoon Bernhard Hesse Remi Tucoulou Sven Geissler Melanie Ort Georg N. Duda Carsten Perka Georgi I. Wassilew Giorgio Perino Anastasia Rakow Synchrotron-based characterization of arthroprosthetic CoCrMo particles in human bone marrow Journal of Materials Science: Materials in Medicine |
| title | Synchrotron-based characterization of arthroprosthetic CoCrMo particles in human bone marrow |
| title_full | Synchrotron-based characterization of arthroprosthetic CoCrMo particles in human bone marrow |
| title_fullStr | Synchrotron-based characterization of arthroprosthetic CoCrMo particles in human bone marrow |
| title_full_unstemmed | Synchrotron-based characterization of arthroprosthetic CoCrMo particles in human bone marrow |
| title_short | Synchrotron-based characterization of arthroprosthetic CoCrMo particles in human bone marrow |
| title_sort | synchrotron based characterization of arthroprosthetic cocrmo particles in human bone marrow |
| url | https://doi.org/10.1007/s10856-022-06675-2 |
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