Fabrication of electrospun polyacrylonitrile ion-exchange membranes for application in lysozyme adsorption
Ion exchange (IEX) chromatography is commonly used in separation and purification systems. However, micropore blockage within its resin structure can easily lead to a reduction in the effectiveness of purification. To tackle this problem, we adopted the concept of membrane separation by combining el...
| Format: | Article |
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| Language: | English |
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Budapest University of Technology
2011-04-01
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| Series: | eXPRESS Polymer Letters |
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| Online Access: | http://www.expresspolymlett.com/letolt.php?file=EPL-0001906&mi=cd |
| _version_ | 1818759555940614144 |
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| collection | DOAJ |
| description | Ion exchange (IEX) chromatography is commonly used in separation and purification systems. However, micropore blockage within its resin structure can easily lead to a reduction in the effectiveness of purification. To tackle this problem, we adopted the concept of membrane separation by combining electrospinning techniques with rapid alkaline hydrolysis to prepare a weak acid IEX nanofibrous membrane (AEA-COOH), consisting of polyethyleneterephthalate (PET) meltblown fabric as a supporting layer, with upper and lower IEX layers consisting of polyacrylonitrile (PAN) nanofibrous membranes. To determine the characteristics of the AEA-COOH membrane, we used the commercial product Sartobind© C IEX membrane as the standard of comparison. Results showed that the base weight and thickness of AEACOOH were 33 and 64%, relative to Sartobind© C membrane. The thermo-degradable temperature of AEA-COOH membrane (320°C) was far higher than that of Sartobind© C (115°C), indicating high thermal stability. Finally, comparisons between the lysozyme adsorption rates and capacity of various IEX membranes confirmed that AEA-COOH was lighter, thinner, faster, possessing higher protein adsorption efficiency than Sartobind© C membrane. |
| first_indexed | 2024-12-18T06:44:36Z |
| format | Article |
| id | doaj.art-343b3e5c9fa740c885954a9d4de7e00e |
| institution | Directory Open Access Journal |
| issn | 1788-618X |
| language | English |
| last_indexed | 2024-12-18T06:44:36Z |
| publishDate | 2011-04-01 |
| publisher | Budapest University of Technology |
| record_format | Article |
| series | eXPRESS Polymer Letters |
| spelling | doaj.art-343b3e5c9fa740c885954a9d4de7e00e2022-12-21T21:17:31ZengBudapest University of TechnologyeXPRESS Polymer Letters1788-618X2011-04-015430831710.3144/expresspolymlett.2011.31Fabrication of electrospun polyacrylonitrile ion-exchange membranes for application in lysozyme adsorptionIon exchange (IEX) chromatography is commonly used in separation and purification systems. However, micropore blockage within its resin structure can easily lead to a reduction in the effectiveness of purification. To tackle this problem, we adopted the concept of membrane separation by combining electrospinning techniques with rapid alkaline hydrolysis to prepare a weak acid IEX nanofibrous membrane (AEA-COOH), consisting of polyethyleneterephthalate (PET) meltblown fabric as a supporting layer, with upper and lower IEX layers consisting of polyacrylonitrile (PAN) nanofibrous membranes. To determine the characteristics of the AEA-COOH membrane, we used the commercial product Sartobind© C IEX membrane as the standard of comparison. Results showed that the base weight and thickness of AEACOOH were 33 and 64%, relative to Sartobind© C membrane. The thermo-degradable temperature of AEA-COOH membrane (320°C) was far higher than that of Sartobind© C (115°C), indicating high thermal stability. Finally, comparisons between the lysozyme adsorption rates and capacity of various IEX membranes confirmed that AEA-COOH was lighter, thinner, faster, possessing higher protein adsorption efficiency than Sartobind© C membrane.http://www.expresspolymlett.com/letolt.php?file=EPL-0001906&mi=cdPolymer membraneselectrospunnanofiberion-exchangelysozymes adsorption |
| spellingShingle | Fabrication of electrospun polyacrylonitrile ion-exchange membranes for application in lysozyme adsorption eXPRESS Polymer Letters Polymer membranes electrospun nanofiber ion-exchange lysozymes adsorption |
| title | Fabrication of electrospun polyacrylonitrile ion-exchange membranes for application in lysozyme adsorption |
| title_full | Fabrication of electrospun polyacrylonitrile ion-exchange membranes for application in lysozyme adsorption |
| title_fullStr | Fabrication of electrospun polyacrylonitrile ion-exchange membranes for application in lysozyme adsorption |
| title_full_unstemmed | Fabrication of electrospun polyacrylonitrile ion-exchange membranes for application in lysozyme adsorption |
| title_short | Fabrication of electrospun polyacrylonitrile ion-exchange membranes for application in lysozyme adsorption |
| title_sort | fabrication of electrospun polyacrylonitrile ion exchange membranes for application in lysozyme adsorption |
| topic | Polymer membranes electrospun nanofiber ion-exchange lysozymes adsorption |
| url | http://www.expresspolymlett.com/letolt.php?file=EPL-0001906&mi=cd |