Blood-Derived Extracellular Vesicle-Associated miR-3182 Detects Non-Small Cell Lung Cancer Patients
With five-year survival rates as low as 3%, lung cancer is the most common cause of cancer-related mortality worldwide. The severity of the disease at presentation is accredited to the lack of early detection capacities, resulting in the reliance on low-throughput diagnostic measures, such as tissue...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/1/257 |
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| author | Kekoolani S. Visan Richard J. Lobb Shu Wen Wen Justin Bedo Luize G. Lima Sophie Krumeich Carlos Palma Kaltin Ferguson Ben Green Colleen Niland Nicole Cloonan Peter T. Simpson Amy E. McCart Reed Sarah J. Everitt Michael P. MacManus Gunter Hartel Carlos Salomon Sunil R. Lakhani David Fielding Andreas Möller |
| author_facet | Kekoolani S. Visan Richard J. Lobb Shu Wen Wen Justin Bedo Luize G. Lima Sophie Krumeich Carlos Palma Kaltin Ferguson Ben Green Colleen Niland Nicole Cloonan Peter T. Simpson Amy E. McCart Reed Sarah J. Everitt Michael P. MacManus Gunter Hartel Carlos Salomon Sunil R. Lakhani David Fielding Andreas Möller |
| author_sort | Kekoolani S. Visan |
| collection | DOAJ |
| description | With five-year survival rates as low as 3%, lung cancer is the most common cause of cancer-related mortality worldwide. The severity of the disease at presentation is accredited to the lack of early detection capacities, resulting in the reliance on low-throughput diagnostic measures, such as tissue biopsy and imaging. Interest in the development and use of liquid biopsies has risen, due to non-invasive sample collection, and the depth of information it can provide on a disease. Small extracellular vesicles (sEVs) as viable liquid biopsies are of particular interest due to their potential as cancer biomarkers. To validate the use of sEVs as cancer biomarkers, we characterised cancer sEVs using miRNA sequencing analysis. We found that miRNA-3182 was highly enriched in sEVs derived from the blood of patients with invasive breast carcinoma and NSCLC. The enrichment of sEV miR-3182 was confirmed in oncogenic, transformed lung cells in comparison to isogenic, untransformed lung cells. Most importantly, miR-3182 can successfully distinguish early-stage NSCLC patients from those with benign lung conditions. Therefore, miR-3182 provides potential to be used for the detection of NSCLC in blood samples, which could result in earlier therapy and thus improved outcomes and survival for patients. |
| first_indexed | 2024-03-10T03:46:11Z |
| format | Article |
| id | doaj.art-343f1b316dd74d5cbf4acb36f05e6f4e |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T03:46:11Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-343f1b316dd74d5cbf4acb36f05e6f4e2023-11-23T11:18:38ZengMDPI AGCancers2072-66942022-01-0114125710.3390/cancers14010257Blood-Derived Extracellular Vesicle-Associated miR-3182 Detects Non-Small Cell Lung Cancer PatientsKekoolani S. Visan0Richard J. Lobb1Shu Wen Wen2Justin Bedo3Luize G. Lima4Sophie Krumeich5Carlos Palma6Kaltin Ferguson7Ben Green8Colleen Niland9Nicole Cloonan10Peter T. Simpson11Amy E. McCart Reed12Sarah J. Everitt13Michael P. MacManus14Gunter Hartel15Carlos Salomon16Sunil R. Lakhani17David Fielding18Andreas Möller19Tumour Microenvironment Laboratory, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, AustraliaTumour Microenvironment Laboratory, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, AustraliaCentre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences, Monash University, Clayton, VIC 3168, AustraliaBioinformatics Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, AustraliaTumour Microenvironment Laboratory, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, AustraliaTumour Microenvironment Laboratory, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, AustraliaExosome Biology Laboratory, Centre for Clinical Diagnostics, UQ Centre for Clinical Research, Royal Brisbane and Women’s Hospital, Faculty of Medicine, The University of Queensland, Brisbane QLD 4029, AustraliaUQ Centre for Clinical Research, The University of Queensland, Brisbane, QLD 4072, AustraliaUQ Centre for Clinical Research, The University of Queensland, Brisbane, QLD 4072, AustraliaUQ Centre for Clinical Research, The University of Queensland, Brisbane, QLD 4072, AustraliaFaculty of Science, University of Auckland, Auckland 1010, New ZealandUQ Centre for Clinical Research, The University of Queensland, Brisbane, QLD 4072, AustraliaUQ Centre for Clinical Research, The University of Queensland, Brisbane, QLD 4072, AustraliaDivision of Radiation Oncology, Peter MacCallum Cancer Centre, Melbourne, VIC 3000, AustraliaDivision of Radiation Oncology, Peter MacCallum Cancer Centre, Melbourne, VIC 3000, AustraliaStatistics Unit, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, AustraliaExosome Biology Laboratory, Centre for Clinical Diagnostics, UQ Centre for Clinical Research, Royal Brisbane and Women’s Hospital, Faculty of Medicine, The University of Queensland, Brisbane QLD 4029, AustraliaUQ Centre for Clinical Research, The University of Queensland, Brisbane, QLD 4072, AustraliaUQ Centre for Clinical Research, The University of Queensland, Brisbane, QLD 4072, AustraliaTumour Microenvironment Laboratory, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, AustraliaWith five-year survival rates as low as 3%, lung cancer is the most common cause of cancer-related mortality worldwide. The severity of the disease at presentation is accredited to the lack of early detection capacities, resulting in the reliance on low-throughput diagnostic measures, such as tissue biopsy and imaging. Interest in the development and use of liquid biopsies has risen, due to non-invasive sample collection, and the depth of information it can provide on a disease. Small extracellular vesicles (sEVs) as viable liquid biopsies are of particular interest due to their potential as cancer biomarkers. To validate the use of sEVs as cancer biomarkers, we characterised cancer sEVs using miRNA sequencing analysis. We found that miRNA-3182 was highly enriched in sEVs derived from the blood of patients with invasive breast carcinoma and NSCLC. The enrichment of sEV miR-3182 was confirmed in oncogenic, transformed lung cells in comparison to isogenic, untransformed lung cells. Most importantly, miR-3182 can successfully distinguish early-stage NSCLC patients from those with benign lung conditions. Therefore, miR-3182 provides potential to be used for the detection of NSCLC in blood samples, which could result in earlier therapy and thus improved outcomes and survival for patients.https://www.mdpi.com/2072-6694/14/1/257extracellular vesiclesexosomesbiomarkersliquid biopsymiRNAdiagnosis |
| spellingShingle | Kekoolani S. Visan Richard J. Lobb Shu Wen Wen Justin Bedo Luize G. Lima Sophie Krumeich Carlos Palma Kaltin Ferguson Ben Green Colleen Niland Nicole Cloonan Peter T. Simpson Amy E. McCart Reed Sarah J. Everitt Michael P. MacManus Gunter Hartel Carlos Salomon Sunil R. Lakhani David Fielding Andreas Möller Blood-Derived Extracellular Vesicle-Associated miR-3182 Detects Non-Small Cell Lung Cancer Patients Cancers extracellular vesicles exosomes biomarkers liquid biopsy miRNA diagnosis |
| title | Blood-Derived Extracellular Vesicle-Associated miR-3182 Detects Non-Small Cell Lung Cancer Patients |
| title_full | Blood-Derived Extracellular Vesicle-Associated miR-3182 Detects Non-Small Cell Lung Cancer Patients |
| title_fullStr | Blood-Derived Extracellular Vesicle-Associated miR-3182 Detects Non-Small Cell Lung Cancer Patients |
| title_full_unstemmed | Blood-Derived Extracellular Vesicle-Associated miR-3182 Detects Non-Small Cell Lung Cancer Patients |
| title_short | Blood-Derived Extracellular Vesicle-Associated miR-3182 Detects Non-Small Cell Lung Cancer Patients |
| title_sort | blood derived extracellular vesicle associated mir 3182 detects non small cell lung cancer patients |
| topic | extracellular vesicles exosomes biomarkers liquid biopsy miRNA diagnosis |
| url | https://www.mdpi.com/2072-6694/14/1/257 |
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