Valerian essential oil for treating insomnia via the serotonergic synapse pathway
Valerian volatile oil can be used in the treatment of insomnia; however, the active components and mechanisms of action are currently unclear. Therefore, we used transcriptome sequencing and weight coefficient network pharmacology to predict the effective components and mechanism of action of valeri...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.927434/full |
| _version_ | 1818537257655599104 |
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| author | Wenfei Wang Yichun Wang Qiuting Guo Huiting Li Huiting Li Zhaoqiang Wang Jia Li Taotao Li Tiantian Tang Yujiao Wang Yanzhuo Jia Yao Wang Junbo Zou Yajun Shi Dongyan Guo Ming Yang Xiaofei Zhang Xiaofei Zhang Jing Sun |
| author_facet | Wenfei Wang Yichun Wang Qiuting Guo Huiting Li Huiting Li Zhaoqiang Wang Jia Li Taotao Li Tiantian Tang Yujiao Wang Yanzhuo Jia Yao Wang Junbo Zou Yajun Shi Dongyan Guo Ming Yang Xiaofei Zhang Xiaofei Zhang Jing Sun |
| author_sort | Wenfei Wang |
| collection | DOAJ |
| description | Valerian volatile oil can be used in the treatment of insomnia; however, the active components and mechanisms of action are currently unclear. Therefore, we used transcriptome sequencing and weight coefficient network pharmacology to predict the effective components and mechanism of action of valerian volatile oil in an insomnia model induced by intraperitoneal injection of para-Chlorophenylalanine (PCPA) in SD rats. Valerian essential oil was given orally for treatment and the contents of 5-hydroxytryptamine receptor 1 A (5-HT1AR), γ-aminobutyric acid (GABA), cyclic adenosine monophosphate (cAMP), and protein kinase A (PKA) in the hippocampus of rats in each group were detected by enzyme-linked immunosorbent assay (ELISA), western blot, Polymerase Chain Reaction (PCR), and immunohistochemistry. The results showed that after treatment with valerian essential oil, insomnia rats showed significantly prolonged sleep duration and alleviated insomnia-induced tension and anxiety. Regarding the mechanism of action, we believe that caryophyllene in valerian essential oil upregulates the 5-HT1AR receptor to improve the activity or affinity of the central transmitter 5-HT, increase the release of 5-HT, couple 5-HT with a G protein coupled receptor, convert adenosine triphosphate (ATP) into cAMP (catalyzed by ADCY5), and then directly regulate the downstream pathway. Following pathway activation, we propose that the core gene protein kinase PKA activates the serotonergic synapse signal pathway to increase the expression of 5-HT and GABA, thus improving insomnia symptoms and alleviating anxiety. This study provides a theoretical basis for the application of valerian volatile oil in health food. |
| first_indexed | 2024-12-11T18:48:27Z |
| format | Article |
| id | doaj.art-34453562e6224d90a9c65cd0e23cf273 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-11T18:48:27Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-34453562e6224d90a9c65cd0e23cf2732022-12-22T00:54:22ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-07-01910.3389/fnut.2022.927434927434Valerian essential oil for treating insomnia via the serotonergic synapse pathwayWenfei Wang0Yichun Wang1Qiuting Guo2Huiting Li3Huiting Li4Zhaoqiang Wang5Jia Li6Taotao Li7Tiantian Tang8Yujiao Wang9Yanzhuo Jia10Yao Wang11Junbo Zou12Yajun Shi13Dongyan Guo14Ming Yang15Xiaofei Zhang16Xiaofei Zhang17Jing Sun18Key Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaSchool of Pharmaceutical Sciences, College of Pharmacy, Kyushu University, Fukuoka, JapanXianyang Vocational Technical College, College of Pharmacy, Xianyang, ChinaKey Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaKey Laboratory of Modern Preparation of TCM, Ministry of Education, College of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang, ChinaShaanxi Haitian Pharmaceutical Co., Ltd., Xianyang, ChinaKey Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaKey Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaKey Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaKey Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaKey Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaKey Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaKey Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaKey Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaKey Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaXianyang Vocational Technical College, College of Pharmacy, Xianyang, ChinaKey Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaKey Laboratory of Modern Preparation of TCM, Ministry of Education, College of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang, ChinaKey Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, ChinaValerian volatile oil can be used in the treatment of insomnia; however, the active components and mechanisms of action are currently unclear. Therefore, we used transcriptome sequencing and weight coefficient network pharmacology to predict the effective components and mechanism of action of valerian volatile oil in an insomnia model induced by intraperitoneal injection of para-Chlorophenylalanine (PCPA) in SD rats. Valerian essential oil was given orally for treatment and the contents of 5-hydroxytryptamine receptor 1 A (5-HT1AR), γ-aminobutyric acid (GABA), cyclic adenosine monophosphate (cAMP), and protein kinase A (PKA) in the hippocampus of rats in each group were detected by enzyme-linked immunosorbent assay (ELISA), western blot, Polymerase Chain Reaction (PCR), and immunohistochemistry. The results showed that after treatment with valerian essential oil, insomnia rats showed significantly prolonged sleep duration and alleviated insomnia-induced tension and anxiety. Regarding the mechanism of action, we believe that caryophyllene in valerian essential oil upregulates the 5-HT1AR receptor to improve the activity or affinity of the central transmitter 5-HT, increase the release of 5-HT, couple 5-HT with a G protein coupled receptor, convert adenosine triphosphate (ATP) into cAMP (catalyzed by ADCY5), and then directly regulate the downstream pathway. Following pathway activation, we propose that the core gene protein kinase PKA activates the serotonergic synapse signal pathway to increase the expression of 5-HT and GABA, thus improving insomnia symptoms and alleviating anxiety. This study provides a theoretical basis for the application of valerian volatile oil in health food.https://www.frontiersin.org/articles/10.3389/fnut.2022.927434/fullinsomniavaleriantranscriptome sequencingweight coefficientmechanism |
| spellingShingle | Wenfei Wang Yichun Wang Qiuting Guo Huiting Li Huiting Li Zhaoqiang Wang Jia Li Taotao Li Tiantian Tang Yujiao Wang Yanzhuo Jia Yao Wang Junbo Zou Yajun Shi Dongyan Guo Ming Yang Xiaofei Zhang Xiaofei Zhang Jing Sun Valerian essential oil for treating insomnia via the serotonergic synapse pathway Frontiers in Nutrition insomnia valerian transcriptome sequencing weight coefficient mechanism |
| title | Valerian essential oil for treating insomnia via the serotonergic synapse pathway |
| title_full | Valerian essential oil for treating insomnia via the serotonergic synapse pathway |
| title_fullStr | Valerian essential oil for treating insomnia via the serotonergic synapse pathway |
| title_full_unstemmed | Valerian essential oil for treating insomnia via the serotonergic synapse pathway |
| title_short | Valerian essential oil for treating insomnia via the serotonergic synapse pathway |
| title_sort | valerian essential oil for treating insomnia via the serotonergic synapse pathway |
| topic | insomnia valerian transcriptome sequencing weight coefficient mechanism |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.927434/full |
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