Accuracy and reliability of a videogrammetry system in the analysis of displacement of Wistar rats
Abstract Aims The behavior of laboratory animals has been studied through displacement, with different objectives by researchers. Methods Although different methods have already been used in the tracking of laboratory animals, manual tracking mode videogrammetry for 2D analysis of displacement has...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual Paulista (UNESP)
2017-07-01
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| Series: | Motriz: Revista de Educacao Fisica |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1980-65742017000300302&lng=en&tlng=en |
| _version_ | 1818037946793590784 |
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| author | Osvaldo Tadeu da Silva Junior Paulo Roberto Santiago Jose Alexandre Almeida Leme Curiacos Julio Wilson dos Santos |
| author_facet | Osvaldo Tadeu da Silva Junior Paulo Roberto Santiago Jose Alexandre Almeida Leme Curiacos Julio Wilson dos Santos |
| author_sort | Osvaldo Tadeu da Silva Junior |
| collection | DOAJ |
| description | Abstract Aims The behavior of laboratory animals has been studied through displacement, with different objectives by researchers. Methods Although different methods have already been used in the tracking of laboratory animals, manual tracking mode videogrammetry for 2D analysis of displacement has not been observed in animal studies. The aim of this study was to verify the accuracy and reliability of determining the displacement of Wistar rats by means of videogrammetry software Dvideo. The accuracy (between the known distance and distance traced) was determined by 3 different evaluators twice consecutive, by videoing a course of 10 meters in the enriched environment together with further analysis of the displacement of the midpoint marked on an apparatus. To calculate reliability (accuracy of the measurement system) and reproducibility (precision of the evaluators) to obtain the ratio of precision to tolerance (P/T), eight animals were filmed for 10 minutes in the enriched environment and analysis of distance covered by one of the animals by three different reviewers thrice consecutive. Results The results obtained in the course of 10 meters of the known distance demonstrated accuracy of 0.10 m, precision of 0.05 m, and bias of 0.07 m. In the reliability test during the 10 minutes of displacement (m) of animal, the ratio of precision to tolerance (P/T) = 0.1 m was found between three different evaluators, demonstrating adequate capacity of the measure. Conclusion The manual tracking mode of the Dvideo presented high reliability and it can be employed for the displacement analysis of studies with rat experimental models. |
| first_indexed | 2024-12-10T07:34:56Z |
| format | Article |
| id | doaj.art-34473bde84bd40658067e38407381ba1 |
| institution | Directory Open Access Journal |
| issn | 1980-6574 |
| language | English |
| last_indexed | 2024-12-10T07:34:56Z |
| publishDate | 2017-07-01 |
| publisher | Universidade Estadual Paulista (UNESP) |
| record_format | Article |
| series | Motriz: Revista de Educacao Fisica |
| spelling | doaj.art-34473bde84bd40658067e38407381ba12022-12-22T01:57:27ZengUniversidade Estadual Paulista (UNESP)Motriz: Revista de Educacao Fisica1980-65742017-07-0123310.1590/s1980-6574201700030006S1980-65742017000300302Accuracy and reliability of a videogrammetry system in the analysis of displacement of Wistar ratsOsvaldo Tadeu da Silva JuniorPaulo Roberto SantiagoJose Alexandre Almeida Leme CuriacosJulio Wilson dos SantosAbstract Aims The behavior of laboratory animals has been studied through displacement, with different objectives by researchers. Methods Although different methods have already been used in the tracking of laboratory animals, manual tracking mode videogrammetry for 2D analysis of displacement has not been observed in animal studies. The aim of this study was to verify the accuracy and reliability of determining the displacement of Wistar rats by means of videogrammetry software Dvideo. The accuracy (between the known distance and distance traced) was determined by 3 different evaluators twice consecutive, by videoing a course of 10 meters in the enriched environment together with further analysis of the displacement of the midpoint marked on an apparatus. To calculate reliability (accuracy of the measurement system) and reproducibility (precision of the evaluators) to obtain the ratio of precision to tolerance (P/T), eight animals were filmed for 10 minutes in the enriched environment and analysis of distance covered by one of the animals by three different reviewers thrice consecutive. Results The results obtained in the course of 10 meters of the known distance demonstrated accuracy of 0.10 m, precision of 0.05 m, and bias of 0.07 m. In the reliability test during the 10 minutes of displacement (m) of animal, the ratio of precision to tolerance (P/T) = 0.1 m was found between three different evaluators, demonstrating adequate capacity of the measure. Conclusion The manual tracking mode of the Dvideo presented high reliability and it can be employed for the displacement analysis of studies with rat experimental models.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1980-65742017000300302&lng=en&tlng=endisplacementvideogrammetry2D analysis |
| spellingShingle | Osvaldo Tadeu da Silva Junior Paulo Roberto Santiago Jose Alexandre Almeida Leme Curiacos Julio Wilson dos Santos Accuracy and reliability of a videogrammetry system in the analysis of displacement of Wistar rats Motriz: Revista de Educacao Fisica displacement videogrammetry 2D analysis |
| title | Accuracy and reliability of a videogrammetry system in the analysis of displacement of Wistar rats |
| title_full | Accuracy and reliability of a videogrammetry system in the analysis of displacement of Wistar rats |
| title_fullStr | Accuracy and reliability of a videogrammetry system in the analysis of displacement of Wistar rats |
| title_full_unstemmed | Accuracy and reliability of a videogrammetry system in the analysis of displacement of Wistar rats |
| title_short | Accuracy and reliability of a videogrammetry system in the analysis of displacement of Wistar rats |
| title_sort | accuracy and reliability of a videogrammetry system in the analysis of displacement of wistar rats |
| topic | displacement videogrammetry 2D analysis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1980-65742017000300302&lng=en&tlng=en |
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