Alterations in Skeletal Muscle mRNA Abundance in Response to Ethyl-Cellulose Rumen-Protected Methionine during the Periparturient Period in Dairy Cows
This study aimed to evaluate the effect of feeding ethyl cellulose rumen-protected methionine (RPM) on skeletal muscle mRNA abundance during the periparturient period. Sixty multiparous Holstein cows were used in a block design and assigned to either a control or RPM diet. The RPM was supplied from...
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MDPI AG
2022-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/13/1641 |
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| author | Lam Phuoc Thanh Qianming Jiang Nithat Wichasit Fernanda Batistel Claudia Parys Jessie Guyader Juan J. Loor |
| author_facet | Lam Phuoc Thanh Qianming Jiang Nithat Wichasit Fernanda Batistel Claudia Parys Jessie Guyader Juan J. Loor |
| author_sort | Lam Phuoc Thanh |
| collection | DOAJ |
| description | This study aimed to evaluate the effect of feeding ethyl cellulose rumen-protected methionine (RPM) on skeletal muscle mRNA abundance during the periparturient period. Sixty multiparous Holstein cows were used in a block design and assigned to either a control or RPM diet. The RPM was supplied from −28 to 60 days in milk (DIM) at a rate of 0.09% (prepartum) or 0.10% (postpartum) of dry matter (DM), ensuring a Lys:Met in the metabolizable protein of ~2.8:1. Muscle biopsies were collected at −21, 1, and 21 DIM. Thirty-five target genes associated with nutrient metabolism and biochemical pathways were measured via RT-qPCR. The mRNA abundance of genes associated with amino acid (AA) transport (<i>SLC7A8</i>, <i>SLC43A2</i>), carnitine transport (<i>SLC22A5</i>), insulin signaling (<i>IRS1</i>), and antioxidant response (<i>NFE2L2</i>) had diet × time effect (<i>p</i> < 0.05) due to greater abundance in RPM versus CON cows, especially at 1 and 21 DIM. Members of the AA transport (<i>SLC7A8</i>, <i>SLC25A29</i>, <i>SCL38A9</i>), fatty acid β-oxidation (<i>ACADVL</i>), vitamin transport (<i>SLC5A6</i>, <i>SLC19A2</i>), mTOR pathway (<i>AKT1</i> and <i>mTOR</i>), antioxidant response (<i>KEAP1</i>, <i>CUL3</i>), CDP-Choline pathway and arginine metabolism had overall greater abundance (<i>p</i> < 0.05) in RPM versus CON cows. Overall, data indicate that RPM can alter nutrient metabolism in the skeletal muscle around parturition partly through alterations in mRNA abundance. |
| first_indexed | 2024-03-09T22:10:11Z |
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| id | doaj.art-34479094339844dc9e8495645a7e31f3 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T22:10:11Z |
| publishDate | 2022-06-01 |
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| spelling | doaj.art-34479094339844dc9e8495645a7e31f32023-11-23T19:32:22ZengMDPI AGAnimals2076-26152022-06-011213164110.3390/ani12131641Alterations in Skeletal Muscle mRNA Abundance in Response to Ethyl-Cellulose Rumen-Protected Methionine during the Periparturient Period in Dairy CowsLam Phuoc Thanh0Qianming Jiang1Nithat Wichasit2Fernanda Batistel3Claudia Parys4Jessie Guyader5Juan J. Loor6Department of Animal Sciences, Can Tho University, Ninh Kieu, Can Tho 94000, VietnamDepartment of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USADepartment of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USADepartment of Animal Sciences, University of Florida, Gainesville, FL 32608, USAEvonik Operations GmbH, Hanau-Wolfgang, 63457 Essen, GermanyEvonik Operations GmbH, Hanau-Wolfgang, 63457 Essen, GermanyDepartment of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USAThis study aimed to evaluate the effect of feeding ethyl cellulose rumen-protected methionine (RPM) on skeletal muscle mRNA abundance during the periparturient period. Sixty multiparous Holstein cows were used in a block design and assigned to either a control or RPM diet. The RPM was supplied from −28 to 60 days in milk (DIM) at a rate of 0.09% (prepartum) or 0.10% (postpartum) of dry matter (DM), ensuring a Lys:Met in the metabolizable protein of ~2.8:1. Muscle biopsies were collected at −21, 1, and 21 DIM. Thirty-five target genes associated with nutrient metabolism and biochemical pathways were measured via RT-qPCR. The mRNA abundance of genes associated with amino acid (AA) transport (<i>SLC7A8</i>, <i>SLC43A2</i>), carnitine transport (<i>SLC22A5</i>), insulin signaling (<i>IRS1</i>), and antioxidant response (<i>NFE2L2</i>) had diet × time effect (<i>p</i> < 0.05) due to greater abundance in RPM versus CON cows, especially at 1 and 21 DIM. Members of the AA transport (<i>SLC7A8</i>, <i>SLC25A29</i>, <i>SCL38A9</i>), fatty acid β-oxidation (<i>ACADVL</i>), vitamin transport (<i>SLC5A6</i>, <i>SLC19A2</i>), mTOR pathway (<i>AKT1</i> and <i>mTOR</i>), antioxidant response (<i>KEAP1</i>, <i>CUL3</i>), CDP-Choline pathway and arginine metabolism had overall greater abundance (<i>p</i> < 0.05) in RPM versus CON cows. Overall, data indicate that RPM can alter nutrient metabolism in the skeletal muscle around parturition partly through alterations in mRNA abundance.https://www.mdpi.com/2076-2615/12/13/1641dairy cowsmRNA abundanceperiparturientrumen-protected methionineskeletal muscle |
| spellingShingle | Lam Phuoc Thanh Qianming Jiang Nithat Wichasit Fernanda Batistel Claudia Parys Jessie Guyader Juan J. Loor Alterations in Skeletal Muscle mRNA Abundance in Response to Ethyl-Cellulose Rumen-Protected Methionine during the Periparturient Period in Dairy Cows Animals dairy cows mRNA abundance periparturient rumen-protected methionine skeletal muscle |
| title | Alterations in Skeletal Muscle mRNA Abundance in Response to Ethyl-Cellulose Rumen-Protected Methionine during the Periparturient Period in Dairy Cows |
| title_full | Alterations in Skeletal Muscle mRNA Abundance in Response to Ethyl-Cellulose Rumen-Protected Methionine during the Periparturient Period in Dairy Cows |
| title_fullStr | Alterations in Skeletal Muscle mRNA Abundance in Response to Ethyl-Cellulose Rumen-Protected Methionine during the Periparturient Period in Dairy Cows |
| title_full_unstemmed | Alterations in Skeletal Muscle mRNA Abundance in Response to Ethyl-Cellulose Rumen-Protected Methionine during the Periparturient Period in Dairy Cows |
| title_short | Alterations in Skeletal Muscle mRNA Abundance in Response to Ethyl-Cellulose Rumen-Protected Methionine during the Periparturient Period in Dairy Cows |
| title_sort | alterations in skeletal muscle mrna abundance in response to ethyl cellulose rumen protected methionine during the periparturient period in dairy cows |
| topic | dairy cows mRNA abundance periparturient rumen-protected methionine skeletal muscle |
| url | https://www.mdpi.com/2076-2615/12/13/1641 |
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