MiR-33a targets FOSL1 and EN2 as a clinical prognostic marker for sarcopenia by glioma
To determine the relationship between glioma and muscle aging and to predict prognosis by screening for co-expressed genes, this study examined the relationship between glioma and sarcopenia. The study identified eight co-downregulated miRNAs, three co-upregulated miRNAs, and seven genes associated...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.953580/full |
| _version_ | 1828149003397627904 |
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| author | Wei Wang Wei Liu Jing Xu Hongze Jin |
| author_facet | Wei Wang Wei Liu Jing Xu Hongze Jin |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | To determine the relationship between glioma and muscle aging and to predict prognosis by screening for co-expressed genes, this study examined the relationship between glioma and sarcopenia. The study identified eight co-downregulated miRNAs, three co-upregulated miRNAs, and seven genes associated with overall glioma survival, namely, KRAS, IFNB1, ALCAM, ERBB2, STAT3, FOSL1, and EN2. With a multi-factor Cox regression model incorporating FOSL1 and EN2, we obtained ROC curves of 0.702 and 0.709, respectively, suggesting that glioma prognosis can be predicted by FOSL1 and EN2, which are differentially expressed in both cancer and aged muscle. FOSL1 and EN2 were analyzed using Gene Set Enrichment Analysis to identify possible functional pathways. RT-qPCR and a dual-luciferase reporter gene system verified that hsa-miR-33a targets FOSL1 and EN2. We found that hsa-mir-33a co-targeting FOSL1 and EN2 has a good predictive value for glioblastoma and skeletal muscle reduction. |
| first_indexed | 2024-04-11T21:24:45Z |
| format | Article |
| id | doaj.art-34488a84b5f14b75b2f5a5682c58f19b |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-11T21:24:45Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-34488a84b5f14b75b2f5a5682c58f19b2022-12-22T04:02:29ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-08-011310.3389/fgene.2022.953580953580MiR-33a targets FOSL1 and EN2 as a clinical prognostic marker for sarcopenia by gliomaWei WangWei LiuJing XuHongze JinTo determine the relationship between glioma and muscle aging and to predict prognosis by screening for co-expressed genes, this study examined the relationship between glioma and sarcopenia. The study identified eight co-downregulated miRNAs, three co-upregulated miRNAs, and seven genes associated with overall glioma survival, namely, KRAS, IFNB1, ALCAM, ERBB2, STAT3, FOSL1, and EN2. With a multi-factor Cox regression model incorporating FOSL1 and EN2, we obtained ROC curves of 0.702 and 0.709, respectively, suggesting that glioma prognosis can be predicted by FOSL1 and EN2, which are differentially expressed in both cancer and aged muscle. FOSL1 and EN2 were analyzed using Gene Set Enrichment Analysis to identify possible functional pathways. RT-qPCR and a dual-luciferase reporter gene system verified that hsa-miR-33a targets FOSL1 and EN2. We found that hsa-mir-33a co-targeting FOSL1 and EN2 has a good predictive value for glioblastoma and skeletal muscle reduction.https://www.frontiersin.org/articles/10.3389/fgene.2022.953580/fullFOSL1EN2mir-33agliomasarcopeniamuscle |
| spellingShingle | Wei Wang Wei Liu Jing Xu Hongze Jin MiR-33a targets FOSL1 and EN2 as a clinical prognostic marker for sarcopenia by glioma Frontiers in Genetics FOSL1 EN2 mir-33a glioma sarcopenia muscle |
| title | MiR-33a targets FOSL1 and EN2 as a clinical prognostic marker for sarcopenia by glioma |
| title_full | MiR-33a targets FOSL1 and EN2 as a clinical prognostic marker for sarcopenia by glioma |
| title_fullStr | MiR-33a targets FOSL1 and EN2 as a clinical prognostic marker for sarcopenia by glioma |
| title_full_unstemmed | MiR-33a targets FOSL1 and EN2 as a clinical prognostic marker for sarcopenia by glioma |
| title_short | MiR-33a targets FOSL1 and EN2 as a clinical prognostic marker for sarcopenia by glioma |
| title_sort | mir 33a targets fosl1 and en2 as a clinical prognostic marker for sarcopenia by glioma |
| topic | FOSL1 EN2 mir-33a glioma sarcopenia muscle |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.953580/full |
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