Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication
Ubiquitination and deubiquitination processes are widely involved in modulating the function, activity, localization, and stability of multiple cellular proteins regulating almost every aspect of cellular function. Several virus families have been shown to exploit the cellular ubiquitin-conjugating...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/2/481 |
| _version_ | 1827755103131533312 |
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| author | Lady S. López Eliana P. Calvo Jaime E. Castellanos |
| author_facet | Lady S. López Eliana P. Calvo Jaime E. Castellanos |
| author_sort | Lady S. López |
| collection | DOAJ |
| description | Ubiquitination and deubiquitination processes are widely involved in modulating the function, activity, localization, and stability of multiple cellular proteins regulating almost every aspect of cellular function. Several virus families have been shown to exploit the cellular ubiquitin-conjugating system to achieve a productive infection: enter the cell, promote genome replication, or assemble and release viral progeny. In this study, we analyzed the role of deubiquitinating enzymes (DUBs) during chikungunya virus (CHIKV) infection. HEK293T, Vero-E6, and Huh-7 cells were treated with two DUB inhibitors (PR619 or WP1130). Then, infected cells were evaluated by flow cytometry, and viral progeny was quantified using the plaque assay method. The changes in viral proteins and viral RNA were analyzed using Western blotting and RT-qPCR, respectively. Results indicate that treatment with DUB inhibitors impairs CHIKV replication due to significant protein and viral RNA synthesis deregulation. Therefore, DUB activity may be a pharmacological target for blocking CHIKV infection. |
| first_indexed | 2024-03-11T08:00:31Z |
| format | Article |
| id | doaj.art-344aa8243a0f4cb395d8b65120783bbc |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T08:00:31Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-344aa8243a0f4cb395d8b65120783bbc2023-11-16T23:49:36ZengMDPI AGViruses1999-49152023-02-0115248110.3390/v15020481Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus ReplicationLady S. López0Eliana P. Calvo1Jaime E. Castellanos2Grupo de Virología, Universidad El Bosque, Bogotá 110121, ColombiaGrupo de Virología, Universidad El Bosque, Bogotá 110121, ColombiaGrupo de Virología, Universidad El Bosque, Bogotá 110121, ColombiaUbiquitination and deubiquitination processes are widely involved in modulating the function, activity, localization, and stability of multiple cellular proteins regulating almost every aspect of cellular function. Several virus families have been shown to exploit the cellular ubiquitin-conjugating system to achieve a productive infection: enter the cell, promote genome replication, or assemble and release viral progeny. In this study, we analyzed the role of deubiquitinating enzymes (DUBs) during chikungunya virus (CHIKV) infection. HEK293T, Vero-E6, and Huh-7 cells were treated with two DUB inhibitors (PR619 or WP1130). Then, infected cells were evaluated by flow cytometry, and viral progeny was quantified using the plaque assay method. The changes in viral proteins and viral RNA were analyzed using Western blotting and RT-qPCR, respectively. Results indicate that treatment with DUB inhibitors impairs CHIKV replication due to significant protein and viral RNA synthesis deregulation. Therefore, DUB activity may be a pharmacological target for blocking CHIKV infection.https://www.mdpi.com/1999-4915/15/2/481chikungunya virusinfectionreplicationubiquitinationdeubiquitinating enzymesdeubiquitinase inhibitor |
| spellingShingle | Lady S. López Eliana P. Calvo Jaime E. Castellanos Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication Viruses chikungunya virus infection replication ubiquitination deubiquitinating enzymes deubiquitinase inhibitor |
| title | Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication |
| title_full | Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication |
| title_fullStr | Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication |
| title_full_unstemmed | Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication |
| title_short | Deubiquitinating Enzyme Inhibitors Block Chikungunya Virus Replication |
| title_sort | deubiquitinating enzyme inhibitors block chikungunya virus replication |
| topic | chikungunya virus infection replication ubiquitination deubiquitinating enzymes deubiquitinase inhibitor |
| url | https://www.mdpi.com/1999-4915/15/2/481 |
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