| Summary: | Cystic echinococcosis (CE) is a zoonotic disease and with has a global distribution. Today, much research
carries out to inactivate hydatid cyst protoscoleces. In particular, herbal compounds have received more
attention due to their cheapness, easy access, low toxicity, and side effects. This study aimed to compare the
scolicidal effect of hydroalcoholic extract of Allium sativum (garlic) and Ferula asafoetida (angozeh) on
hydatid cyst protoscoleces in vitro. The scolicidal activity of A. sativum and F. asafoetida extracts were
evaluated at concentrations of 50, 100, 150, 200, and 250 mg/ml following 10, 30, and 60 minutes of exposure.
Each reaction was repeated three times. The viability of protoscoleces was examined with a 0.1% eosin stain
under a light microscope. The chemical composition of two extracts was analyzed using Gas ChromatographyMass Spectrometry (GC–MS). Statistical analyses were performed with GraphPad software version 5.0. The
results of this study showed that F. asafoetida extract, at a concentration of 250 mg/ml after 60 minutes of
exposure, killed 100% of the protoscoleces compared to the control group, but the hydroalcoholic extract of
A. sativum at the same concentration and time, it was able to kill 98% of protoscoleces. The main chemical
components of A. sativum and F. asafoetida identified as allyl methyl trisulfide (12.8%) and methyl ester
(13.9%), respectively. The findings of the present study showed that F. asafoetida has more potent scolicidal
effects than A. sativum. However, further studies are needed to evaluate the effectiveness of the F. asafoetida
plant.
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