Comparison of the scolicidal effect of Allium sativum and Ferula asafoetida extract on hydatid cyst protoscoleces in vitro
Cystic echinococcosis (CE) is a zoonotic disease and with has a global distribution. Today, much research carries out to inactivate hydatid cyst protoscoleces. In particular, herbal compounds have received more attention due to their cheapness, easy access, low toxicity, and side effects. This stu...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of Tabriz
2022-12-01
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| Series: | Journal of Zoonotic Diseases |
| Subjects: | |
| Online Access: | https://jzd.tabrizu.ac.ir/article_14998.html |
| _version_ | 1797953857524858880 |
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| author | Roghayeh Norouzi Amin Mohammadpour Marzie Hejazy Shalaleh Mousavi |
| author_facet | Roghayeh Norouzi Amin Mohammadpour Marzie Hejazy Shalaleh Mousavi |
| author_sort | Roghayeh Norouzi |
| collection | DOAJ |
| description | Cystic echinococcosis (CE) is a zoonotic disease and with has a global distribution. Today, much research
carries out to inactivate hydatid cyst protoscoleces. In particular, herbal compounds have received more
attention due to their cheapness, easy access, low toxicity, and side effects. This study aimed to compare the
scolicidal effect of hydroalcoholic extract of Allium sativum (garlic) and Ferula asafoetida (angozeh) on
hydatid cyst protoscoleces in vitro. The scolicidal activity of A. sativum and F. asafoetida extracts were
evaluated at concentrations of 50, 100, 150, 200, and 250 mg/ml following 10, 30, and 60 minutes of exposure.
Each reaction was repeated three times. The viability of protoscoleces was examined with a 0.1% eosin stain
under a light microscope. The chemical composition of two extracts was analyzed using Gas ChromatographyMass Spectrometry (GC–MS). Statistical analyses were performed with GraphPad software version 5.0. The
results of this study showed that F. asafoetida extract, at a concentration of 250 mg/ml after 60 minutes of
exposure, killed 100% of the protoscoleces compared to the control group, but the hydroalcoholic extract of
A. sativum at the same concentration and time, it was able to kill 98% of protoscoleces. The main chemical
components of A. sativum and F. asafoetida identified as allyl methyl trisulfide (12.8%) and methyl ester
(13.9%), respectively. The findings of the present study showed that F. asafoetida has more potent scolicidal
effects than A. sativum. However, further studies are needed to evaluate the effectiveness of the F. asafoetida
plant. |
| first_indexed | 2024-04-10T23:08:42Z |
| format | Article |
| id | doaj.art-344c8170a6564a688bc074113e24d5be |
| institution | Directory Open Access Journal |
| issn | 2717-2910 |
| language | English |
| last_indexed | 2024-04-10T23:08:42Z |
| publishDate | 2022-12-01 |
| publisher | University of Tabriz |
| record_format | Article |
| series | Journal of Zoonotic Diseases |
| spelling | doaj.art-344c8170a6564a688bc074113e24d5be2023-01-13T07:05:34ZengUniversity of TabrizJournal of Zoonotic Diseases2717-29102022-12-016416817610.22034/JZD.2022.14998Comparison of the scolicidal effect of Allium sativum and Ferula asafoetida extract on hydatid cyst protoscoleces in vitroRoghayeh Norouzi0 Amin Mohammadpour 1Marzie Hejazy2Shalaleh Mousavi3Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranDepartment of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranDepartment of Food Hygiene and Aquatic Animals, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranCystic echinococcosis (CE) is a zoonotic disease and with has a global distribution. Today, much research carries out to inactivate hydatid cyst protoscoleces. In particular, herbal compounds have received more attention due to their cheapness, easy access, low toxicity, and side effects. This study aimed to compare the scolicidal effect of hydroalcoholic extract of Allium sativum (garlic) and Ferula asafoetida (angozeh) on hydatid cyst protoscoleces in vitro. The scolicidal activity of A. sativum and F. asafoetida extracts were evaluated at concentrations of 50, 100, 150, 200, and 250 mg/ml following 10, 30, and 60 minutes of exposure. Each reaction was repeated three times. The viability of protoscoleces was examined with a 0.1% eosin stain under a light microscope. The chemical composition of two extracts was analyzed using Gas ChromatographyMass Spectrometry (GC–MS). Statistical analyses were performed with GraphPad software version 5.0. The results of this study showed that F. asafoetida extract, at a concentration of 250 mg/ml after 60 minutes of exposure, killed 100% of the protoscoleces compared to the control group, but the hydroalcoholic extract of A. sativum at the same concentration and time, it was able to kill 98% of protoscoleces. The main chemical components of A. sativum and F. asafoetida identified as allyl methyl trisulfide (12.8%) and methyl ester (13.9%), respectively. The findings of the present study showed that F. asafoetida has more potent scolicidal effects than A. sativum. However, further studies are needed to evaluate the effectiveness of the F. asafoetida plant. https://jzd.tabrizu.ac.ir/article_14998.htmlhydatid cystscolicidalallium sativumferula asafoetidain vitro |
| spellingShingle | Roghayeh Norouzi Amin Mohammadpour Marzie Hejazy Shalaleh Mousavi Comparison of the scolicidal effect of Allium sativum and Ferula asafoetida extract on hydatid cyst protoscoleces in vitro Journal of Zoonotic Diseases hydatid cyst scolicidal allium sativum ferula asafoetida in vitro |
| title | Comparison of the scolicidal effect of Allium sativum and Ferula asafoetida extract on hydatid cyst protoscoleces in vitro |
| title_full | Comparison of the scolicidal effect of Allium sativum and Ferula asafoetida extract on hydatid cyst protoscoleces in vitro |
| title_fullStr | Comparison of the scolicidal effect of Allium sativum and Ferula asafoetida extract on hydatid cyst protoscoleces in vitro |
| title_full_unstemmed | Comparison of the scolicidal effect of Allium sativum and Ferula asafoetida extract on hydatid cyst protoscoleces in vitro |
| title_short | Comparison of the scolicidal effect of Allium sativum and Ferula asafoetida extract on hydatid cyst protoscoleces in vitro |
| title_sort | comparison of the scolicidal effect of allium sativum and ferula asafoetida extract on hydatid cyst protoscoleces in vitro |
| topic | hydatid cyst scolicidal allium sativum ferula asafoetida in vitro |
| url | https://jzd.tabrizu.ac.ir/article_14998.html |
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