Urinary MicroRNAs as Potential Markers for Non-Invasive Diagnosis of Bladder Cancer
Currently, voided urine cytology (VUC) serves as the gold standard for the detection of bladder cancer (BCa) in urine. Despite its high specificity, VUC has shortcomings in terms of sensitivity. Therefore, alternative biomarkers are being searched, which might overcome these disadvantages as a usefu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/11/3814 |
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| author | Kati Erdmann Karsten Salomo Anna Klimova Ulrike Heberling Andrea Lohse-Fischer Romy Fuehrer Christian Thomas Ingo Roeder Michael Froehner Manfred P. Wirth Susanne Fuessel |
| author_facet | Kati Erdmann Karsten Salomo Anna Klimova Ulrike Heberling Andrea Lohse-Fischer Romy Fuehrer Christian Thomas Ingo Roeder Michael Froehner Manfred P. Wirth Susanne Fuessel |
| author_sort | Kati Erdmann |
| collection | DOAJ |
| description | Currently, voided urine cytology (VUC) serves as the gold standard for the detection of bladder cancer (BCa) in urine. Despite its high specificity, VUC has shortcomings in terms of sensitivity. Therefore, alternative biomarkers are being searched, which might overcome these disadvantages as a useful adjunct to VUC. The aim of this study was to evaluate the diagnostic potential of the urinary levels of selected microRNAs (miRs), which might represent such alternative biomarkers due to their BCa-specific expression. Expression levels of nine BCa-associated microRNAs (miR-21, -96, -125b, -126, -145, -183, -205, -210, -221) were assessed by quantitative PCR in urine sediments from 104 patients with primary BCa and 46 control subjects. Receiver operating characteristic (ROC) curve analyses revealed a diagnostic potential for miR-96, -125b, -126, -145, -183, and -221 with area under the curve (AUC) values between 0.605 and 0.772. The combination of the four best candidates resulted in sensitivity, specificity, positive and negative predictive values (NPV), and accuracy of 73.1%, 95.7%, 97.4%, 61.1%, and 80.0%, respectively. Combined with VUC, sensitivity and NPV could be increased by nearly 8%, each surpassing the performance of VUC alone. The present findings suggested a diagnostic potential of miR-125b, -145, -183, and -221 in combination with VUC for non-invasive detection of BCa in urine. |
| first_indexed | 2024-03-10T19:32:30Z |
| format | Article |
| id | doaj.art-344f31e90ec64853b817c60fc0e82983 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:32:30Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-344f31e90ec64853b817c60fc0e829832023-11-20T01:57:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-05-012111381410.3390/ijms21113814Urinary MicroRNAs as Potential Markers for Non-Invasive Diagnosis of Bladder CancerKati Erdmann0Karsten Salomo1Anna Klimova2Ulrike Heberling3Andrea Lohse-Fischer4Romy Fuehrer5Christian Thomas6Ingo Roeder7Michael Froehner8Manfred P. Wirth9Susanne Fuessel10Department of Urology, Technische Universität Dresden, 01307 Dresden, GermanyDepartment of Urology, Technische Universität Dresden, 01307 Dresden, GermanyNational Center for Tumor Diseases (NCT), 01307 Dresden, GermanyDepartment of Urology, Technische Universität Dresden, 01307 Dresden, GermanyDepartment of Urology, Technische Universität Dresden, 01307 Dresden, GermanyDepartment of Urology, Technische Universität Dresden, 01307 Dresden, GermanyDepartment of Urology, Technische Universität Dresden, 01307 Dresden, GermanyNational Center for Tumor Diseases (NCT), 01307 Dresden, GermanyDepartment of Urology, Technische Universität Dresden, 01307 Dresden, GermanyDepartment of Urology, Technische Universität Dresden, 01307 Dresden, GermanyDepartment of Urology, Technische Universität Dresden, 01307 Dresden, GermanyCurrently, voided urine cytology (VUC) serves as the gold standard for the detection of bladder cancer (BCa) in urine. Despite its high specificity, VUC has shortcomings in terms of sensitivity. Therefore, alternative biomarkers are being searched, which might overcome these disadvantages as a useful adjunct to VUC. The aim of this study was to evaluate the diagnostic potential of the urinary levels of selected microRNAs (miRs), which might represent such alternative biomarkers due to their BCa-specific expression. Expression levels of nine BCa-associated microRNAs (miR-21, -96, -125b, -126, -145, -183, -205, -210, -221) were assessed by quantitative PCR in urine sediments from 104 patients with primary BCa and 46 control subjects. Receiver operating characteristic (ROC) curve analyses revealed a diagnostic potential for miR-96, -125b, -126, -145, -183, and -221 with area under the curve (AUC) values between 0.605 and 0.772. The combination of the four best candidates resulted in sensitivity, specificity, positive and negative predictive values (NPV), and accuracy of 73.1%, 95.7%, 97.4%, 61.1%, and 80.0%, respectively. Combined with VUC, sensitivity and NPV could be increased by nearly 8%, each surpassing the performance of VUC alone. The present findings suggested a diagnostic potential of miR-125b, -145, -183, and -221 in combination with VUC for non-invasive detection of BCa in urine.https://www.mdpi.com/1422-0067/21/11/3814miRNAquantitative PCRtumor markerurothelial carcinomavoided urine cytology |
| spellingShingle | Kati Erdmann Karsten Salomo Anna Klimova Ulrike Heberling Andrea Lohse-Fischer Romy Fuehrer Christian Thomas Ingo Roeder Michael Froehner Manfred P. Wirth Susanne Fuessel Urinary MicroRNAs as Potential Markers for Non-Invasive Diagnosis of Bladder Cancer International Journal of Molecular Sciences miRNA quantitative PCR tumor marker urothelial carcinoma voided urine cytology |
| title | Urinary MicroRNAs as Potential Markers for Non-Invasive Diagnosis of Bladder Cancer |
| title_full | Urinary MicroRNAs as Potential Markers for Non-Invasive Diagnosis of Bladder Cancer |
| title_fullStr | Urinary MicroRNAs as Potential Markers for Non-Invasive Diagnosis of Bladder Cancer |
| title_full_unstemmed | Urinary MicroRNAs as Potential Markers for Non-Invasive Diagnosis of Bladder Cancer |
| title_short | Urinary MicroRNAs as Potential Markers for Non-Invasive Diagnosis of Bladder Cancer |
| title_sort | urinary micrornas as potential markers for non invasive diagnosis of bladder cancer |
| topic | miRNA quantitative PCR tumor marker urothelial carcinoma voided urine cytology |
| url | https://www.mdpi.com/1422-0067/21/11/3814 |
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