Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry—A Perspective Study
Tumor dissemination is one of the most-investigated steps of tumor progression, which in recent decades led to the rapid development of liquid biopsy aiming to analyze circulating tumor cells (CTCs), extracellular vesicles (EVs), and circulating nucleic acids in order to precisely diagnose and monit...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/14/17/4178 |
| _version_ | 1797496088076222464 |
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| author | Anna Muchlińska Julia Smentoch Anna J. Żaczek Natalia Bednarz-Knoll |
| author_facet | Anna Muchlińska Julia Smentoch Anna J. Żaczek Natalia Bednarz-Knoll |
| author_sort | Anna Muchlińska |
| collection | DOAJ |
| description | Tumor dissemination is one of the most-investigated steps of tumor progression, which in recent decades led to the rapid development of liquid biopsy aiming to analyze circulating tumor cells (CTCs), extracellular vesicles (EVs), and circulating nucleic acids in order to precisely diagnose and monitor cancer patients. Flow cytometry was considered as a method to detect CTCs; however, due to the lack of verification of the investigated cells’ identity, this method failed to reach clinical utility. Meanwhile, imaging flow cytometry combining the sensitivity and high throughput of flow cytometry and image-based detailed analysis through a high-resolution microscope might open a new avenue in CTC technologies and provide an open-platform system alternative to CellSearch<sup>®</sup>, which is still the only gold standard in this field. Hereby, we shortly review the studies on the usage of flow cytometry in CTC identification and present our own representative images of CTCs envisioned by imaging flow cytometry providing rationale that this novel technology might be a good tool for studying tumor dissemination, and, if combined with a high CTC yield enrichment method, could upgrade CTC-based diagnostics. |
| first_indexed | 2024-03-10T01:58:39Z |
| format | Article |
| id | doaj.art-34514cd5e17b4940af2f9ebb3ef2d31c |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T01:58:39Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-34514cd5e17b4940af2f9ebb3ef2d31c2023-11-23T12:51:09ZengMDPI AGCancers2072-66942022-08-011417417810.3390/cancers14174178Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry—A Perspective StudyAnna Muchlińska0Julia Smentoch1Anna J. Żaczek2Natalia Bednarz-Knoll3Laboratory of Translational Oncology, Medical University of Gdańsk, 80-210 Gdańsk, PolandLaboratory of Translational Oncology, Medical University of Gdańsk, 80-210 Gdańsk, PolandLaboratory of Translational Oncology, Medical University of Gdańsk, 80-210 Gdańsk, PolandLaboratory of Translational Oncology, Medical University of Gdańsk, 80-210 Gdańsk, PolandTumor dissemination is one of the most-investigated steps of tumor progression, which in recent decades led to the rapid development of liquid biopsy aiming to analyze circulating tumor cells (CTCs), extracellular vesicles (EVs), and circulating nucleic acids in order to precisely diagnose and monitor cancer patients. Flow cytometry was considered as a method to detect CTCs; however, due to the lack of verification of the investigated cells’ identity, this method failed to reach clinical utility. Meanwhile, imaging flow cytometry combining the sensitivity and high throughput of flow cytometry and image-based detailed analysis through a high-resolution microscope might open a new avenue in CTC technologies and provide an open-platform system alternative to CellSearch<sup>®</sup>, which is still the only gold standard in this field. Hereby, we shortly review the studies on the usage of flow cytometry in CTC identification and present our own representative images of CTCs envisioned by imaging flow cytometry providing rationale that this novel technology might be a good tool for studying tumor dissemination, and, if combined with a high CTC yield enrichment method, could upgrade CTC-based diagnostics.https://www.mdpi.com/2072-6694/14/17/4178liquid biopsycirculating tumor cellsimaging flow cytometry |
| spellingShingle | Anna Muchlińska Julia Smentoch Anna J. Żaczek Natalia Bednarz-Knoll Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry—A Perspective Study Cancers liquid biopsy circulating tumor cells imaging flow cytometry |
| title | Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry—A Perspective Study |
| title_full | Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry—A Perspective Study |
| title_fullStr | Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry—A Perspective Study |
| title_full_unstemmed | Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry—A Perspective Study |
| title_short | Detection and Characterization of Circulating Tumor Cells Using Imaging Flow Cytometry—A Perspective Study |
| title_sort | detection and characterization of circulating tumor cells using imaging flow cytometry a perspective study |
| topic | liquid biopsy circulating tumor cells imaging flow cytometry |
| url | https://www.mdpi.com/2072-6694/14/17/4178 |
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