Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging
Background: Panax notoginseng is a highly valued medicinal herb used widely in China and many Asian countries. Its root and rhizome have long been used for the treatment of cardiovascular and hematological diseases. Imaging the spatial distributions and dynamics of metabolites in heterogeneous plant...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-11-01
|
| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845321000439 |
| _version_ | 1818825046129377280 |
|---|---|
| author | Chenglong Sun Shuangshuang Ma Lili Li Daijie Wang Wei Liu Feng Liu Lanping Guo Xiao Wang |
| author_facet | Chenglong Sun Shuangshuang Ma Lili Li Daijie Wang Wei Liu Feng Liu Lanping Guo Xiao Wang |
| author_sort | Chenglong Sun |
| collection | DOAJ |
| description | Background: Panax notoginseng is a highly valued medicinal herb used widely in China and many Asian countries. Its root and rhizome have long been used for the treatment of cardiovascular and hematological diseases. Imaging the spatial distributions and dynamics of metabolites in heterogeneous plant tissues is significant for characterizing the metabolic networks of Panax notoginseng, and this will also provide a highly informative approach to understand the complex molecular changes in the processing of Panax notoginseng. Methods: Here, a high-sensitive MALDI-MS imaging method was developed and adopted to visualize the spatial distributions and spatiotemporal changes of metabolites in different botanical parts of Panax notoginseng. Results: A wide spectrum of metabolites including notoginsenosides, ginsenosides, amino acids, dencichine, gluconic acid, and low-molecular-weight organic acids were imaged in Panax notoginseng rhizome and root tissues for the first time. Moreover, the spatiotemporal alterations of metabolites during the steaming of Panax notoginseng root were also characterized in this study. And, a series of metabolites such as dencichine, arginine and glutamine that changed with the steaming of Panax notoginseng were successfully screened out and imaged. Conclusion: These spatially-resolved metabolite data not only enhance our understanding of the Panax notoginseng metabolic networks, but also provide direct evidence that a serious of metabolic alterations occurred during the steaming of Panax notoginseng. |
| first_indexed | 2024-12-19T00:05:32Z |
| format | Article |
| id | doaj.art-34577e57b54e4fd2a7372fc4e64b6b1e |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-12-19T00:05:32Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-34577e57b54e4fd2a7372fc4e64b6b1e2022-12-21T20:46:14ZengElsevierJournal of Ginseng Research1226-84532021-11-01456726733Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imagingChenglong Sun0Shuangshuang Ma1Lili Li2Daijie Wang3Wei Liu4Feng Liu5Lanping Guo6Xiao Wang7School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China; Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaKey Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaSchool of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China; Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaSchool of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China; Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaSchool of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China; Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaSchool of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China; Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaResource Center of Chinese Materia Medica, State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing, ChinaSchool of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China; Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China; Corresponding author. School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China.Background: Panax notoginseng is a highly valued medicinal herb used widely in China and many Asian countries. Its root and rhizome have long been used for the treatment of cardiovascular and hematological diseases. Imaging the spatial distributions and dynamics of metabolites in heterogeneous plant tissues is significant for characterizing the metabolic networks of Panax notoginseng, and this will also provide a highly informative approach to understand the complex molecular changes in the processing of Panax notoginseng. Methods: Here, a high-sensitive MALDI-MS imaging method was developed and adopted to visualize the spatial distributions and spatiotemporal changes of metabolites in different botanical parts of Panax notoginseng. Results: A wide spectrum of metabolites including notoginsenosides, ginsenosides, amino acids, dencichine, gluconic acid, and low-molecular-weight organic acids were imaged in Panax notoginseng rhizome and root tissues for the first time. Moreover, the spatiotemporal alterations of metabolites during the steaming of Panax notoginseng root were also characterized in this study. And, a series of metabolites such as dencichine, arginine and glutamine that changed with the steaming of Panax notoginseng were successfully screened out and imaged. Conclusion: These spatially-resolved metabolite data not only enhance our understanding of the Panax notoginseng metabolic networks, but also provide direct evidence that a serious of metabolic alterations occurred during the steaming of Panax notoginseng.http://www.sciencedirect.com/science/article/pii/S1226845321000439Panax notoginsengMetabolitesSpatial distributionSteaming processMALDI-MS imaging |
| spellingShingle | Chenglong Sun Shuangshuang Ma Lili Li Daijie Wang Wei Liu Feng Liu Lanping Guo Xiao Wang Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging Journal of Ginseng Research Panax notoginseng Metabolites Spatial distribution Steaming process MALDI-MS imaging |
| title | Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging |
| title_full | Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging |
| title_fullStr | Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging |
| title_full_unstemmed | Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging |
| title_short | Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging |
| title_sort | visualizing the distributions and spatiotemporal changes of metabolites in panax notoginseng by maldi mass spectrometry imaging |
| topic | Panax notoginseng Metabolites Spatial distribution Steaming process MALDI-MS imaging |
| url | http://www.sciencedirect.com/science/article/pii/S1226845321000439 |
| work_keys_str_mv | AT chenglongsun visualizingthedistributionsandspatiotemporalchangesofmetabolitesinpanaxnotoginsengbymaldimassspectrometryimaging AT shuangshuangma visualizingthedistributionsandspatiotemporalchangesofmetabolitesinpanaxnotoginsengbymaldimassspectrometryimaging AT lilili visualizingthedistributionsandspatiotemporalchangesofmetabolitesinpanaxnotoginsengbymaldimassspectrometryimaging AT daijiewang visualizingthedistributionsandspatiotemporalchangesofmetabolitesinpanaxnotoginsengbymaldimassspectrometryimaging AT weiliu visualizingthedistributionsandspatiotemporalchangesofmetabolitesinpanaxnotoginsengbymaldimassspectrometryimaging AT fengliu visualizingthedistributionsandspatiotemporalchangesofmetabolitesinpanaxnotoginsengbymaldimassspectrometryimaging AT lanpingguo visualizingthedistributionsandspatiotemporalchangesofmetabolitesinpanaxnotoginsengbymaldimassspectrometryimaging AT xiaowang visualizingthedistributionsandspatiotemporalchangesofmetabolitesinpanaxnotoginsengbymaldimassspectrometryimaging |